Aktivitas residu protein Cry1Ac Pada lahan yang ditanami kapas transgenik-Bt di Bajeng dan Soppeng, Sulawesi Selatan

<p>Soil samples were collected from each plot at the two field sites at 0, 1, 3, and 5 months after planting, and at one-month intervals after harvest for three months. Soil suspension from each sample was mixed with agar based diet (1 g soil/20 ml soil-diet mixture) then was presented to neon...

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Autores principales:	Purnama Hidayat, Djoko Prijono
Formato:	article
Lenguaje:	EN ID
Publicado:	The Entomological Society of Indonesia 2017
Materias:	Transgenic-Bt, Cry1AC, Kapas, Sulawesi Selatan, Helicoverpa armigera Zoology QL1-991
Acceso en línea:	https://doaj.org/article/9e10fba9686b484b9140308c13fe14d3
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spelling	oai:doaj.org-article:9e10fba9686b484b9140308c13fe14d32021-12-02T08:56:00ZAktivitas residu protein Cry1Ac Pada lahan yang ditanami kapas transgenik-Bt di Bajeng dan Soppeng, Sulawesi Selatan1829-77222089-025710.5994/jei.3.1.50https://doaj.org/article/9e10fba9686b484b9140308c13fe14d32017-02-01T00:00:00Zhttp://jurnal.pei-pusat.org/index.php/jei/article/view/318https://doaj.org/toc/1829-7722https://doaj.org/toc/2089-0257<p>Soil samples were collected from each plot at the two field sites at 0, 1, 3, and 5 months after planting, and at one-month intervals after harvest for three months. Soil suspension from each sample was mixed with agar based diet (1 g soil/20 ml soil-diet mixture) then was presented to neonate larvae of <em>H. armigera</em>. Two reference standards were used, i.e. pure Cry1Ac protein (0.165 x 104 to 1.436 x 105 ng per ml of the proteindiet mixture) and non-Bt-soil spiked with Cry1Ac protein (0.11 x 104 to 9.573 x 104 ng per ml of the soil-diet-mixture). Larval mortality was recorded at 7 days of treatment. In both reference standards, mortality of <em>H. armigera</em> larvae occurred in a concentrationdependent fashion, i.e. larval mortality increased with the increase in Cry1Ac protein concentration, with LC50 of 0.95 x 104 and 1.65 x 104 ng/ml, respectively, for the pure protein and spiked non-Bt-soil standards. Result of the bioassay of soil samples from the field sites showed the residues from both transgenic cotton soils did not cause any mortality (0%) in the test insects. This indicates that the concentrations of Cry1Ac residue in soil at the study sites were very low (in the second standard, Cry1Ac at a concentration of 0.33 x 104 ng/ml caused about 10% larval mortality).</p>Purnama HidayatDjoko PrijonoThe Entomological Society of IndonesiaarticleTransgenic-Bt, Cry1AC, Kapas, Sulawesi Selatan, Helicoverpa armigeraZoologyQL1-991ENIDJurnal Entomologi Indonesia, Vol 3, Iss 1 (2017)
institution	DOAJ
collection	DOAJ
language	EN ID
topic	Transgenic-Bt, Cry1AC, Kapas, Sulawesi Selatan, Helicoverpa armigera Zoology QL1-991
spellingShingle	Transgenic-Bt, Cry1AC, Kapas, Sulawesi Selatan, Helicoverpa armigera Zoology QL1-991 Purnama Hidayat Djoko Prijono Aktivitas residu protein Cry1Ac Pada lahan yang ditanami kapas transgenik-Bt di Bajeng dan Soppeng, Sulawesi Selatan
description	<p>Soil samples were collected from each plot at the two field sites at 0, 1, 3, and 5 months after planting, and at one-month intervals after harvest for three months. Soil suspension from each sample was mixed with agar based diet (1 g soil/20 ml soil-diet mixture) then was presented to neonate larvae of <em>H. armigera</em>. Two reference standards were used, i.e. pure Cry1Ac protein (0.165 x 104 to 1.436 x 105 ng per ml of the proteindiet mixture) and non-Bt-soil spiked with Cry1Ac protein (0.11 x 104 to 9.573 x 104 ng per ml of the soil-diet-mixture). Larval mortality was recorded at 7 days of treatment. In both reference standards, mortality of <em>H. armigera</em> larvae occurred in a concentrationdependent fashion, i.e. larval mortality increased with the increase in Cry1Ac protein concentration, with LC50 of 0.95 x 104 and 1.65 x 104 ng/ml, respectively, for the pure protein and spiked non-Bt-soil standards. Result of the bioassay of soil samples from the field sites showed the residues from both transgenic cotton soils did not cause any mortality (0%) in the test insects. This indicates that the concentrations of Cry1Ac residue in soil at the study sites were very low (in the second standard, Cry1Ac at a concentration of 0.33 x 104 ng/ml caused about 10% larval mortality).</p>
format	article
author	Purnama Hidayat Djoko Prijono
author_facet	Purnama Hidayat Djoko Prijono
author_sort	Purnama Hidayat
title	Aktivitas residu protein Cry1Ac Pada lahan yang ditanami kapas transgenik-Bt di Bajeng dan Soppeng, Sulawesi Selatan
title_short	Aktivitas residu protein Cry1Ac Pada lahan yang ditanami kapas transgenik-Bt di Bajeng dan Soppeng, Sulawesi Selatan
title_full	Aktivitas residu protein Cry1Ac Pada lahan yang ditanami kapas transgenik-Bt di Bajeng dan Soppeng, Sulawesi Selatan
title_fullStr	Aktivitas residu protein Cry1Ac Pada lahan yang ditanami kapas transgenik-Bt di Bajeng dan Soppeng, Sulawesi Selatan
title_full_unstemmed	Aktivitas residu protein Cry1Ac Pada lahan yang ditanami kapas transgenik-Bt di Bajeng dan Soppeng, Sulawesi Selatan
title_sort	aktivitas residu protein cry1ac pada lahan yang ditanami kapas transgenik-bt di bajeng dan soppeng, sulawesi selatan
publisher	The Entomological Society of Indonesia
publishDate	2017
url	https://doaj.org/article/9e10fba9686b484b9140308c13fe14d3
work_keys_str_mv	AT purnamahidayat aktivitasresiduproteincry1acpadalahanyangditanamikapastransgenikbtdibajengdansoppengsulawesiselatan AT djokoprijono aktivitasresiduproteincry1acpadalahanyangditanamikapastransgenikbtdibajengdansoppengsulawesiselatan
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Aktivitas residu protein Cry1Ac Pada lahan yang ditanami kapas transgenik-Bt di Bajeng dan Soppeng, Sulawesi Selatan

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