MALAT1 promotes malignant pleural mesothelioma by sponging miR-141-3p

The aim of this study was to clarify the role of lncRNA metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) in proliferation, migration, and invasion of malignant pleural mesothelioma (MPM) cells. The quantitative reverse transcription polymerase chain reaction (RT-qPCR) was used to dete...

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Autores principales:	Wang Pei, Bai Cuiwei, Shen Shasha, Jiang Chang, Deng Jie, Han Dan
Formato:	article
Lenguaje:	EN
Publicado:	De Gruyter 2021
Materias:	lncrna malat1 malignant pleural mesothelioma mir-141-3p yap1-hippo signaling pathway Medicine R
Acceso en línea:	https://doaj.org/article/9e1202569e884597b3c912d823f753da
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spelling	oai:doaj.org-article:9e1202569e884597b3c912d823f753da2021-12-05T14:10:55ZMALAT1 promotes malignant pleural mesothelioma by sponging miR-141-3p2391-546310.1515/med-2021-0383https://doaj.org/article/9e1202569e884597b3c912d823f753da2021-11-01T00:00:00Zhttps://doi.org/10.1515/med-2021-0383https://doaj.org/toc/2391-5463The aim of this study was to clarify the role of lncRNA metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) in proliferation, migration, and invasion of malignant pleural mesothelioma (MPM) cells. The quantitative reverse transcription polymerase chain reaction (RT-qPCR) was used to detect the expression of MALAT1 in MPM cell lines. The effects of MALAT1 and miR-141-3p on the proliferation, migration, and invasion of MPM cells were studied through a series of in vitro cellular experiments. The flow cytometry was utilized to detect the cell apoptosis. The dual‐luciferase reporter assay was employed to explore the binding relationship among MALAT1, miR-141-3p, and YES-associated protein 1 (YAP1). MALAT1 was overexpressed in MPM cell lines, while its knockdown significantly inhibited the cell proliferation, migration, and invasion, and increased the number of MPM cells in the G0/G1 phase. In addition, MALAT1 could directly bind to miR-141-3p and inhibit its expression. YAP1 has been identified as a downstream target of miR-141-3p, and its expression level was inhibited by miR-141-3p. MALAT1 can be used as a competitive endogenous RNA (ceRNA) to regulate the YAP1-Hippo signaling pathway through miR-141-3p, promote the proliferation, migration, and invasion of MPM cells, and provide a new target for the therapy of MPM.Wang PeiBai CuiweiShen ShashaJiang ChangDeng JieHan DanDe Gruyterarticlelncrna malat1malignant pleural mesotheliomamir-141-3pyap1-hipposignaling pathwayMedicineRENOpen Medicine, Vol 16, Iss 1, Pp 1653-1667 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	lncrna malat1 malignant pleural mesothelioma mir-141-3p yap1-hippo signaling pathway Medicine R
spellingShingle	lncrna malat1 malignant pleural mesothelioma mir-141-3p yap1-hippo signaling pathway Medicine R Wang Pei Bai Cuiwei Shen Shasha Jiang Chang Deng Jie Han Dan MALAT1 promotes malignant pleural mesothelioma by sponging miR-141-3p
description	The aim of this study was to clarify the role of lncRNA metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) in proliferation, migration, and invasion of malignant pleural mesothelioma (MPM) cells. The quantitative reverse transcription polymerase chain reaction (RT-qPCR) was used to detect the expression of MALAT1 in MPM cell lines. The effects of MALAT1 and miR-141-3p on the proliferation, migration, and invasion of MPM cells were studied through a series of in vitro cellular experiments. The flow cytometry was utilized to detect the cell apoptosis. The dual‐luciferase reporter assay was employed to explore the binding relationship among MALAT1, miR-141-3p, and YES-associated protein 1 (YAP1). MALAT1 was overexpressed in MPM cell lines, while its knockdown significantly inhibited the cell proliferation, migration, and invasion, and increased the number of MPM cells in the G0/G1 phase. In addition, MALAT1 could directly bind to miR-141-3p and inhibit its expression. YAP1 has been identified as a downstream target of miR-141-3p, and its expression level was inhibited by miR-141-3p. MALAT1 can be used as a competitive endogenous RNA (ceRNA) to regulate the YAP1-Hippo signaling pathway through miR-141-3p, promote the proliferation, migration, and invasion of MPM cells, and provide a new target for the therapy of MPM.
format	article
author	Wang Pei Bai Cuiwei Shen Shasha Jiang Chang Deng Jie Han Dan
author_facet	Wang Pei Bai Cuiwei Shen Shasha Jiang Chang Deng Jie Han Dan
author_sort	Wang Pei
title	MALAT1 promotes malignant pleural mesothelioma by sponging miR-141-3p
title_short	MALAT1 promotes malignant pleural mesothelioma by sponging miR-141-3p
title_full	MALAT1 promotes malignant pleural mesothelioma by sponging miR-141-3p
title_fullStr	MALAT1 promotes malignant pleural mesothelioma by sponging miR-141-3p
title_full_unstemmed	MALAT1 promotes malignant pleural mesothelioma by sponging miR-141-3p
title_sort	malat1 promotes malignant pleural mesothelioma by sponging mir-141-3p
publisher	De Gruyter
publishDate	2021
url	https://doaj.org/article/9e1202569e884597b3c912d823f753da
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MALAT1 promotes malignant pleural mesothelioma by sponging miR-141-3p

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