Action potential initiation in neocortical inhibitory interneurons.

Action potential (AP) generation in inhibitory interneurons is critical for cortical excitation-inhibition balance and information processing. However, it remains unclear what determines AP initiation in different interneurons. We focused on two predominant interneuron types in neocortex: parvalbumi...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Tun Li, Cuiping Tian, Paolo Scalmani, Carolina Frassoni, Massimo Mantegazza, Yonghong Wang, Mingpo Yang, Si Wu, Yousheng Shu
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/9e15afc91a904a89990ef230a7cb696b
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:9e15afc91a904a89990ef230a7cb696b
record_format	dspace
spelling	oai:doaj.org-article:9e15afc91a904a89990ef230a7cb696b2021-11-25T05:32:58ZAction potential initiation in neocortical inhibitory interneurons.1544-91731545-788510.1371/journal.pbio.1001944https://doaj.org/article/9e15afc91a904a89990ef230a7cb696b2014-09-01T00:00:00Zhttps://doi.org/10.1371/journal.pbio.1001944https://doaj.org/toc/1544-9173https://doaj.org/toc/1545-7885Action potential (AP) generation in inhibitory interneurons is critical for cortical excitation-inhibition balance and information processing. However, it remains unclear what determines AP initiation in different interneurons. We focused on two predominant interneuron types in neocortex: parvalbumin (PV)- and somatostatin (SST)-expressing neurons. Patch-clamp recording from mouse prefrontal cortical slices showed that axonal but not somatic Na+ channels exhibit different voltage-dependent properties. The minimal activation voltage of axonal channels in SST was substantially higher (∼7 mV) than in PV cells, consistent with differences in AP thresholds. A more mixed distribution of high- and low-threshold channel subtypes at the axon initial segment (AIS) of SST cells may lead to these differences. Surprisingly, NaV1.2 was found accumulated at AIS of SST but not PV cells; reducing NaV1.2-mediated currents in interneurons promoted recurrent network activity. Together, our results reveal the molecular identity of axonal Na+ channels in interneurons and their contribution to AP generation and regulation of network activity.Tun LiCuiping TianPaolo ScalmaniCarolina FrassoniMassimo MantegazzaYonghong WangMingpo YangSi WuYousheng ShuPublic Library of Science (PLoS)articleBiology (General)QH301-705.5ENPLoS Biology, Vol 12, Iss 9, p e1001944 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Tun Li Cuiping Tian Paolo Scalmani Carolina Frassoni Massimo Mantegazza Yonghong Wang Mingpo Yang Si Wu Yousheng Shu Action potential initiation in neocortical inhibitory interneurons.
description	Action potential (AP) generation in inhibitory interneurons is critical for cortical excitation-inhibition balance and information processing. However, it remains unclear what determines AP initiation in different interneurons. We focused on two predominant interneuron types in neocortex: parvalbumin (PV)- and somatostatin (SST)-expressing neurons. Patch-clamp recording from mouse prefrontal cortical slices showed that axonal but not somatic Na+ channels exhibit different voltage-dependent properties. The minimal activation voltage of axonal channels in SST was substantially higher (∼7 mV) than in PV cells, consistent with differences in AP thresholds. A more mixed distribution of high- and low-threshold channel subtypes at the axon initial segment (AIS) of SST cells may lead to these differences. Surprisingly, NaV1.2 was found accumulated at AIS of SST but not PV cells; reducing NaV1.2-mediated currents in interneurons promoted recurrent network activity. Together, our results reveal the molecular identity of axonal Na+ channels in interneurons and their contribution to AP generation and regulation of network activity.
format	article
author	Tun Li Cuiping Tian Paolo Scalmani Carolina Frassoni Massimo Mantegazza Yonghong Wang Mingpo Yang Si Wu Yousheng Shu
author_facet	Tun Li Cuiping Tian Paolo Scalmani Carolina Frassoni Massimo Mantegazza Yonghong Wang Mingpo Yang Si Wu Yousheng Shu
author_sort	Tun Li
title	Action potential initiation in neocortical inhibitory interneurons.
title_short	Action potential initiation in neocortical inhibitory interneurons.
title_full	Action potential initiation in neocortical inhibitory interneurons.
title_fullStr	Action potential initiation in neocortical inhibitory interneurons.
title_full_unstemmed	Action potential initiation in neocortical inhibitory interneurons.
title_sort	action potential initiation in neocortical inhibitory interneurons.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/9e15afc91a904a89990ef230a7cb696b
work_keys_str_mv	AT tunli actionpotentialinitiationinneocorticalinhibitoryinterneurons AT cuipingtian actionpotentialinitiationinneocorticalinhibitoryinterneurons AT paoloscalmani actionpotentialinitiationinneocorticalinhibitoryinterneurons AT carolinafrassoni actionpotentialinitiationinneocorticalinhibitoryinterneurons AT massimomantegazza actionpotentialinitiationinneocorticalinhibitoryinterneurons AT yonghongwang actionpotentialinitiationinneocorticalinhibitoryinterneurons AT mingpoyang actionpotentialinitiationinneocorticalinhibitoryinterneurons AT siwu actionpotentialinitiationinneocorticalinhibitoryinterneurons AT youshengshu actionpotentialinitiationinneocorticalinhibitoryinterneurons
_version_	1718414654318837760

Action potential initiation in neocortical inhibitory interneurons.

Ejemplares similares