Infarct growth precedes cerebral thrombosis following experimental stroke in mice

Abstract Ischemic stroke is among the leading causes of disability and death worldwide. In acute ischemic stroke, successful recanalization of occluded vessels is the primary therapeutic aim, but even if it is achieved, not all patients benefit. Although blockade of platelet aggregation did not prev...

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Autores principales:	Vanessa Göb, Maximilian G. Voll, Lena Zimmermann, Katherina Hemmen, Guido Stoll, Bernhard Nieswandt, Michael K. Schuhmann, Katrin G. Heinze, David Stegner
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9e1757261a4d4ba1a3a35180f427cda9
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spelling	oai:doaj.org-article:9e1757261a4d4ba1a3a35180f427cda92021-11-28T12:16:07ZInfarct growth precedes cerebral thrombosis following experimental stroke in mice10.1038/s41598-021-02360-62045-2322https://doaj.org/article/9e1757261a4d4ba1a3a35180f427cda92021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-02360-6https://doaj.org/toc/2045-2322Abstract Ischemic stroke is among the leading causes of disability and death worldwide. In acute ischemic stroke, successful recanalization of occluded vessels is the primary therapeutic aim, but even if it is achieved, not all patients benefit. Although blockade of platelet aggregation did not prevent infarct progression, cerebral thrombosis as cause of secondary infarct growth has remained a matter of debate. As cerebral thrombi are frequently observed after experimental stroke, a thrombus-induced impairment of the brain microcirculation is considered to contribute to tissue damage. Here, we combine the model of transient middle cerebral artery occlusion (tMCAO) with light sheet fluorescence microscopy and immunohistochemistry of brain slices to investigate the kinetics of thrombus formation and infarct progression. Our data reveal that tissue damage already peaks after 8 h of reperfusion following 60 min MCAO, while cerebral thrombi are only observed at later time points. Thus, cerebral thrombosis is not causative for secondary infarct growth during ischemic stroke.Vanessa GöbMaximilian G. VollLena ZimmermannKatherina HemmenGuido StollBernhard NieswandtMichael K. SchuhmannKatrin G. HeinzeDavid StegnerNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-7 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Vanessa Göb Maximilian G. Voll Lena Zimmermann Katherina Hemmen Guido Stoll Bernhard Nieswandt Michael K. Schuhmann Katrin G. Heinze David Stegner Infarct growth precedes cerebral thrombosis following experimental stroke in mice
description	Abstract Ischemic stroke is among the leading causes of disability and death worldwide. In acute ischemic stroke, successful recanalization of occluded vessels is the primary therapeutic aim, but even if it is achieved, not all patients benefit. Although blockade of platelet aggregation did not prevent infarct progression, cerebral thrombosis as cause of secondary infarct growth has remained a matter of debate. As cerebral thrombi are frequently observed after experimental stroke, a thrombus-induced impairment of the brain microcirculation is considered to contribute to tissue damage. Here, we combine the model of transient middle cerebral artery occlusion (tMCAO) with light sheet fluorescence microscopy and immunohistochemistry of brain slices to investigate the kinetics of thrombus formation and infarct progression. Our data reveal that tissue damage already peaks after 8 h of reperfusion following 60 min MCAO, while cerebral thrombi are only observed at later time points. Thus, cerebral thrombosis is not causative for secondary infarct growth during ischemic stroke.
format	article
author	Vanessa Göb Maximilian G. Voll Lena Zimmermann Katherina Hemmen Guido Stoll Bernhard Nieswandt Michael K. Schuhmann Katrin G. Heinze David Stegner
author_facet	Vanessa Göb Maximilian G. Voll Lena Zimmermann Katherina Hemmen Guido Stoll Bernhard Nieswandt Michael K. Schuhmann Katrin G. Heinze David Stegner
author_sort	Vanessa Göb
title	Infarct growth precedes cerebral thrombosis following experimental stroke in mice
title_short	Infarct growth precedes cerebral thrombosis following experimental stroke in mice
title_full	Infarct growth precedes cerebral thrombosis following experimental stroke in mice
title_fullStr	Infarct growth precedes cerebral thrombosis following experimental stroke in mice
title_full_unstemmed	Infarct growth precedes cerebral thrombosis following experimental stroke in mice
title_sort	infarct growth precedes cerebral thrombosis following experimental stroke in mice
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/9e1757261a4d4ba1a3a35180f427cda9
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Infarct growth precedes cerebral thrombosis following experimental stroke in mice

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