Reconstitution of prospermatogonial specification in vitro from human induced pluripotent stem cells

Spermatogonia establishment in the fetal and postnatal period is essential for spermatozoa production. Here the authors present a protocol for in vitro reconstitution of human prospermatogonial specification and perform single cell RNA-sequencing to delineate lineage trajectories.

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Autores principales:	Young Sun Hwang, Shinnosuke Suzuki, Yasunari Seita, Jumpei Ito, Yuka Sakata, Hirofumi Aso, Kei Sato, Brian P. Hermann, Kotaro Sasaki
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/9e1796a5969343aa9d6112aa30c1ceec
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spelling	oai:doaj.org-article:9e1796a5969343aa9d6112aa30c1ceec2021-12-02T16:49:15ZReconstitution of prospermatogonial specification in vitro from human induced pluripotent stem cells10.1038/s41467-020-19350-32041-1723https://doaj.org/article/9e1796a5969343aa9d6112aa30c1ceec2020-11-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-19350-3https://doaj.org/toc/2041-1723Spermatogonia establishment in the fetal and postnatal period is essential for spermatozoa production. Here the authors present a protocol for in vitro reconstitution of human prospermatogonial specification and perform single cell RNA-sequencing to delineate lineage trajectories.Young Sun HwangShinnosuke SuzukiYasunari SeitaJumpei ItoYuka SakataHirofumi AsoKei SatoBrian P. HermannKotaro SasakiNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-17 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Young Sun Hwang Shinnosuke Suzuki Yasunari Seita Jumpei Ito Yuka Sakata Hirofumi Aso Kei Sato Brian P. Hermann Kotaro Sasaki Reconstitution of prospermatogonial specification in vitro from human induced pluripotent stem cells
description	Spermatogonia establishment in the fetal and postnatal period is essential for spermatozoa production. Here the authors present a protocol for in vitro reconstitution of human prospermatogonial specification and perform single cell RNA-sequencing to delineate lineage trajectories.
format	article
author	Young Sun Hwang Shinnosuke Suzuki Yasunari Seita Jumpei Ito Yuka Sakata Hirofumi Aso Kei Sato Brian P. Hermann Kotaro Sasaki
author_facet	Young Sun Hwang Shinnosuke Suzuki Yasunari Seita Jumpei Ito Yuka Sakata Hirofumi Aso Kei Sato Brian P. Hermann Kotaro Sasaki
author_sort	Young Sun Hwang
title	Reconstitution of prospermatogonial specification in vitro from human induced pluripotent stem cells
title_short	Reconstitution of prospermatogonial specification in vitro from human induced pluripotent stem cells
title_full	Reconstitution of prospermatogonial specification in vitro from human induced pluripotent stem cells
title_fullStr	Reconstitution of prospermatogonial specification in vitro from human induced pluripotent stem cells
title_full_unstemmed	Reconstitution of prospermatogonial specification in vitro from human induced pluripotent stem cells
title_sort	reconstitution of prospermatogonial specification in vitro from human induced pluripotent stem cells
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/9e1796a5969343aa9d6112aa30c1ceec
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Reconstitution of prospermatogonial specification in vitro from human induced pluripotent stem cells

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