Dynamic Mechano-Regulation of Myoblast Cells on Supramolecular Hydrogels Cross-Linked by Reversible Host-Guest Interactions

Abstract A new class of supramolecular hydrogels, cross-linked by host-guest interactions between β-cyclodextrin (βCD) and adamantane, were designed for the dynamic regulation of cell-substrate interactions. The initial substrate elasticity can be optimized by selecting the molar fraction of host- a...

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Autores principales:	Marcel Hörning, Masaki Nakahata, Philipp Linke, Akihisa Yamamoto, Mariam Veschgini, Stefan Kaufmann, Yoshinori Takashima, Akira Harada, Motomu Tanaka
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9e1d796cfb034b8d90862689127f969b
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spelling	oai:doaj.org-article:9e1d796cfb034b8d90862689127f969b2021-12-02T15:05:15ZDynamic Mechano-Regulation of Myoblast Cells on Supramolecular Hydrogels Cross-Linked by Reversible Host-Guest Interactions10.1038/s41598-017-07934-x2045-2322https://doaj.org/article/9e1d796cfb034b8d90862689127f969b2017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07934-xhttps://doaj.org/toc/2045-2322Abstract A new class of supramolecular hydrogels, cross-linked by host-guest interactions between β-cyclodextrin (βCD) and adamantane, were designed for the dynamic regulation of cell-substrate interactions. The initial substrate elasticity can be optimized by selecting the molar fraction of host- and guest monomers for the target cells. Moreover, owing to the reversible nature of host-guest interactions, the magnitude of softening and stiffening of the substrate can be modulated by varying the concentrations of free, competing host molecules (βCD) in solutions. By changing the substrate elasticity at a desired time point, it is possible to switch the micromechanical environments of cells. We demonstrated that the Young’s modulus of our “host-guest gels”, 4–11 kPa, lies in an optimal range not only for static (ex situ) but also for dynamic (in situ) regulation of cell morphology and cytoskeletal ordering of myoblasts. Compared to other stimulus-responsive materials that can either change the elasticity only in one direction or rely on less biocompatible stimuli such as UV light and temperature change, our supramolecular hydrogel enables to reversibly apply mechanical cues to various cell types in vitro without interfering cell viability.Marcel HörningMasaki NakahataPhilipp LinkeAkihisa YamamotoMariam VeschginiStefan KaufmannYoshinori TakashimaAkira HaradaMotomu TanakaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Marcel Hörning Masaki Nakahata Philipp Linke Akihisa Yamamoto Mariam Veschgini Stefan Kaufmann Yoshinori Takashima Akira Harada Motomu Tanaka Dynamic Mechano-Regulation of Myoblast Cells on Supramolecular Hydrogels Cross-Linked by Reversible Host-Guest Interactions
description	Abstract A new class of supramolecular hydrogels, cross-linked by host-guest interactions between β-cyclodextrin (βCD) and adamantane, were designed for the dynamic regulation of cell-substrate interactions. The initial substrate elasticity can be optimized by selecting the molar fraction of host- and guest monomers for the target cells. Moreover, owing to the reversible nature of host-guest interactions, the magnitude of softening and stiffening of the substrate can be modulated by varying the concentrations of free, competing host molecules (βCD) in solutions. By changing the substrate elasticity at a desired time point, it is possible to switch the micromechanical environments of cells. We demonstrated that the Young’s modulus of our “host-guest gels”, 4–11 kPa, lies in an optimal range not only for static (ex situ) but also for dynamic (in situ) regulation of cell morphology and cytoskeletal ordering of myoblasts. Compared to other stimulus-responsive materials that can either change the elasticity only in one direction or rely on less biocompatible stimuli such as UV light and temperature change, our supramolecular hydrogel enables to reversibly apply mechanical cues to various cell types in vitro without interfering cell viability.
format	article
author	Marcel Hörning Masaki Nakahata Philipp Linke Akihisa Yamamoto Mariam Veschgini Stefan Kaufmann Yoshinori Takashima Akira Harada Motomu Tanaka
author_facet	Marcel Hörning Masaki Nakahata Philipp Linke Akihisa Yamamoto Mariam Veschgini Stefan Kaufmann Yoshinori Takashima Akira Harada Motomu Tanaka
author_sort	Marcel Hörning
title	Dynamic Mechano-Regulation of Myoblast Cells on Supramolecular Hydrogels Cross-Linked by Reversible Host-Guest Interactions
title_short	Dynamic Mechano-Regulation of Myoblast Cells on Supramolecular Hydrogels Cross-Linked by Reversible Host-Guest Interactions
title_full	Dynamic Mechano-Regulation of Myoblast Cells on Supramolecular Hydrogels Cross-Linked by Reversible Host-Guest Interactions
title_fullStr	Dynamic Mechano-Regulation of Myoblast Cells on Supramolecular Hydrogels Cross-Linked by Reversible Host-Guest Interactions
title_full_unstemmed	Dynamic Mechano-Regulation of Myoblast Cells on Supramolecular Hydrogels Cross-Linked by Reversible Host-Guest Interactions
title_sort	dynamic mechano-regulation of myoblast cells on supramolecular hydrogels cross-linked by reversible host-guest interactions
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/9e1d796cfb034b8d90862689127f969b
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Dynamic Mechano-Regulation of Myoblast Cells on Supramolecular Hydrogels Cross-Linked by Reversible Host-Guest Interactions

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