Phytic acid as alternative setting retarder enhanced biological performance of dicalcium phosphate cement in vitro

Abstract Dicalcium phosphate cement preparation requires the addition of setting retarders to meet clinical requirements regarding handling time and processability. Previous studies have focused on the influence of different setting modifiers on material properties such as mechanical performance or...

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Autores principales:	Susanne Meininger, Carina Blum, Martha Schamel, Jake E. Barralet, Anita Ignatius, Uwe Gbureck
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9e1eb48f5fc34e3f94b625c46f0171a2
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spelling	oai:doaj.org-article:9e1eb48f5fc34e3f94b625c46f0171a22021-12-02T12:31:48ZPhytic acid as alternative setting retarder enhanced biological performance of dicalcium phosphate cement in vitro10.1038/s41598-017-00731-62045-2322https://doaj.org/article/9e1eb48f5fc34e3f94b625c46f0171a22017-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00731-6https://doaj.org/toc/2045-2322Abstract Dicalcium phosphate cement preparation requires the addition of setting retarders to meet clinical requirements regarding handling time and processability. Previous studies have focused on the influence of different setting modifiers on material properties such as mechanical performance or injectability, while ignoring their influence on biological cement properties as they are used in low concentrations in the cement pastes and the occurrence of most compounds in human tissues. Here, analyses of both material and biological behavior were carried out on samples with common setting retardants (citric acid, sodium pyrophosphate, sulfuric acid) and novel (phytic acid). Cytocompatibility was evaluated by in vitro tests with osteoblastic (hFOB 1.19) and osteoclastic (RAW 264.7) cells. We found cytocompatibility was better for sodium pyrophosphate and phytic acid with a three-fold cell metabolic activity by WST-1 test, whereas samples set with citric acid showed reduced cell number as well as cell activity. The compressive strength (CS) of cements formed with phytic acid (CS = 13 MPa) were nearly equal to those formed with citric acid (CS = 15 MPa) and approximately threefold higher than for other setting retardants. Due to a proven cytocompatibility and high mechanical strength, phytic acid seems to be a candidate replacement setting retardant for dicalcium phosphate cements.Susanne MeiningerCarina BlumMartha SchamelJake E. BarraletAnita IgnatiusUwe GbureckNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Susanne Meininger Carina Blum Martha Schamel Jake E. Barralet Anita Ignatius Uwe Gbureck Phytic acid as alternative setting retarder enhanced biological performance of dicalcium phosphate cement in vitro
description	Abstract Dicalcium phosphate cement preparation requires the addition of setting retarders to meet clinical requirements regarding handling time and processability. Previous studies have focused on the influence of different setting modifiers on material properties such as mechanical performance or injectability, while ignoring their influence on biological cement properties as they are used in low concentrations in the cement pastes and the occurrence of most compounds in human tissues. Here, analyses of both material and biological behavior were carried out on samples with common setting retardants (citric acid, sodium pyrophosphate, sulfuric acid) and novel (phytic acid). Cytocompatibility was evaluated by in vitro tests with osteoblastic (hFOB 1.19) and osteoclastic (RAW 264.7) cells. We found cytocompatibility was better for sodium pyrophosphate and phytic acid with a three-fold cell metabolic activity by WST-1 test, whereas samples set with citric acid showed reduced cell number as well as cell activity. The compressive strength (CS) of cements formed with phytic acid (CS = 13 MPa) were nearly equal to those formed with citric acid (CS = 15 MPa) and approximately threefold higher than for other setting retardants. Due to a proven cytocompatibility and high mechanical strength, phytic acid seems to be a candidate replacement setting retardant for dicalcium phosphate cements.
format	article
author	Susanne Meininger Carina Blum Martha Schamel Jake E. Barralet Anita Ignatius Uwe Gbureck
author_facet	Susanne Meininger Carina Blum Martha Schamel Jake E. Barralet Anita Ignatius Uwe Gbureck
author_sort	Susanne Meininger
title	Phytic acid as alternative setting retarder enhanced biological performance of dicalcium phosphate cement in vitro
title_short	Phytic acid as alternative setting retarder enhanced biological performance of dicalcium phosphate cement in vitro
title_full	Phytic acid as alternative setting retarder enhanced biological performance of dicalcium phosphate cement in vitro
title_fullStr	Phytic acid as alternative setting retarder enhanced biological performance of dicalcium phosphate cement in vitro
title_full_unstemmed	Phytic acid as alternative setting retarder enhanced biological performance of dicalcium phosphate cement in vitro
title_sort	phytic acid as alternative setting retarder enhanced biological performance of dicalcium phosphate cement in vitro
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/9e1eb48f5fc34e3f94b625c46f0171a2
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Phytic acid as alternative setting retarder enhanced biological performance of dicalcium phosphate cement in vitro

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