Emerging patterns of plasmid-host coevolution that stabilize antibiotic resistance

Abstract Multidrug resistant bacterial pathogens have become a serious global human health threat, and conjugative plasmids are important drivers of the rapid spread of resistance to last-resort antibiotics. Whereas antibiotics have been shown to select for adaptation of resistance plasmids to their...

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Autores principales:	Thibault Stalder, Linda M. Rogers, Chris Renfrow, Hirokazu Yano, Zachary Smith, Eva M. Top
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9e1ee435fb634a37a0239f9f8c104464
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spelling	oai:doaj.org-article:9e1ee435fb634a37a0239f9f8c1044642021-12-02T15:05:32ZEmerging patterns of plasmid-host coevolution that stabilize antibiotic resistance10.1038/s41598-017-04662-02045-2322https://doaj.org/article/9e1ee435fb634a37a0239f9f8c1044642017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-04662-0https://doaj.org/toc/2045-2322Abstract Multidrug resistant bacterial pathogens have become a serious global human health threat, and conjugative plasmids are important drivers of the rapid spread of resistance to last-resort antibiotics. Whereas antibiotics have been shown to select for adaptation of resistance plasmids to their new bacterial hosts, or vice versa, a general evolutionary mechanism has not yet emerged. Here we conducted an experimental evolution study aimed at determining general patterns of plasmid-bacteria evolution. Specifically, we found that a large conjugative resistance plasmid follows the same evolutionary trajectories as its non-conjugative mini-replicon in the same and other species. Furthermore, within a single host–plasmid pair three distinct patterns of adaptive evolution led to increased plasmid persistence: i) mutations in the replication protein gene (trfA1); ii) the acquisition by the resistance plasmid of a transposon from a co-residing plasmid encoding a putative toxin-antitoxin system; iii) a mutation in the host’s global transcriptional regulator gene fur. Since each of these evolutionary solutions individually have been shown to increase plasmid persistence in other plasmid-host pairs, our work points towards common mechanisms of plasmid stabilization. These could become the targets of future alternative drug therapies to slow down the spread of antibiotic resistance.Thibault StalderLinda M. RogersChris RenfrowHirokazu YanoZachary SmithEva M. TopNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Thibault Stalder Linda M. Rogers Chris Renfrow Hirokazu Yano Zachary Smith Eva M. Top Emerging patterns of plasmid-host coevolution that stabilize antibiotic resistance
description	Abstract Multidrug resistant bacterial pathogens have become a serious global human health threat, and conjugative plasmids are important drivers of the rapid spread of resistance to last-resort antibiotics. Whereas antibiotics have been shown to select for adaptation of resistance plasmids to their new bacterial hosts, or vice versa, a general evolutionary mechanism has not yet emerged. Here we conducted an experimental evolution study aimed at determining general patterns of plasmid-bacteria evolution. Specifically, we found that a large conjugative resistance plasmid follows the same evolutionary trajectories as its non-conjugative mini-replicon in the same and other species. Furthermore, within a single host–plasmid pair three distinct patterns of adaptive evolution led to increased plasmid persistence: i) mutations in the replication protein gene (trfA1); ii) the acquisition by the resistance plasmid of a transposon from a co-residing plasmid encoding a putative toxin-antitoxin system; iii) a mutation in the host’s global transcriptional regulator gene fur. Since each of these evolutionary solutions individually have been shown to increase plasmid persistence in other plasmid-host pairs, our work points towards common mechanisms of plasmid stabilization. These could become the targets of future alternative drug therapies to slow down the spread of antibiotic resistance.
format	article
author	Thibault Stalder Linda M. Rogers Chris Renfrow Hirokazu Yano Zachary Smith Eva M. Top
author_facet	Thibault Stalder Linda M. Rogers Chris Renfrow Hirokazu Yano Zachary Smith Eva M. Top
author_sort	Thibault Stalder
title	Emerging patterns of plasmid-host coevolution that stabilize antibiotic resistance
title_short	Emerging patterns of plasmid-host coevolution that stabilize antibiotic resistance
title_full	Emerging patterns of plasmid-host coevolution that stabilize antibiotic resistance
title_fullStr	Emerging patterns of plasmid-host coevolution that stabilize antibiotic resistance
title_full_unstemmed	Emerging patterns of plasmid-host coevolution that stabilize antibiotic resistance
title_sort	emerging patterns of plasmid-host coevolution that stabilize antibiotic resistance
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/9e1ee435fb634a37a0239f9f8c104464
work_keys_str_mv	AT thibaultstalder emergingpatternsofplasmidhostcoevolutionthatstabilizeantibioticresistance AT lindamrogers emergingpatternsofplasmidhostcoevolutionthatstabilizeantibioticresistance AT chrisrenfrow emergingpatternsofplasmidhostcoevolutionthatstabilizeantibioticresistance AT hirokazuyano emergingpatternsofplasmidhostcoevolutionthatstabilizeantibioticresistance AT zacharysmith emergingpatternsofplasmidhostcoevolutionthatstabilizeantibioticresistance AT evamtop emergingpatternsofplasmidhostcoevolutionthatstabilizeantibioticresistance
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Emerging patterns of plasmid-host coevolution that stabilize antibiotic resistance

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