Curcumin alleviates diabetic nephropathy via inhibiting podocyte mesenchymal transdifferentiation and inducing autophagy in rats and MPC5 cells

Context: Curcumin could ameliorate diabetic nephropathy (DN), but the mechanism remains unclear. Objective: The efficacy of curcumin on epithelial-to-mesenchymal transition (EMT) of podocyte and autophagy in vivo and in vitro was explored. Materials and methods: Thirty male Sprague–Dawley rats were...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Qiudi Tu, Yiwen Li, Juan Jin, Xinxin Jiang, Yan Ren, Qiang He
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2019
Materias:	glomerular podocytes epithelial-to-mesenchymal transition p13k/akt mtor Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/9e212d5ebc144bfcb89067838fd87d8a
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:9e212d5ebc144bfcb89067838fd87d8a
record_format	dspace
spelling	oai:doaj.org-article:9e212d5ebc144bfcb89067838fd87d8a2021-11-17T14:21:56ZCurcumin alleviates diabetic nephropathy via inhibiting podocyte mesenchymal transdifferentiation and inducing autophagy in rats and MPC5 cells1388-02091744-511610.1080/13880209.2019.1688843https://doaj.org/article/9e212d5ebc144bfcb89067838fd87d8a2019-01-01T00:00:00Zhttp://dx.doi.org/10.1080/13880209.2019.1688843https://doaj.org/toc/1388-0209https://doaj.org/toc/1744-5116Context: Curcumin could ameliorate diabetic nephropathy (DN), but the mechanism remains unclear. Objective: The efficacy of curcumin on epithelial-to-mesenchymal transition (EMT) of podocyte and autophagy in vivo and in vitro was explored. Materials and methods: Thirty male Sprague–Dawley rats were divided into the normal, model and curcumin (300 mg/kg/d, i.g., for 8 weeks) groups. Rats received streptozotocin (50 mg/kg, i.p.) and high-fat-sugar diet to induce DN. Biochemical indicators and histomorphology of renal tissues were observed. In addition, cultured mouse podocytes (MPC5) was induced to EMT with serum from DN rats, and then exposed to curcumin (40 µM) with or without fumonisin B1, an Akt specific activator or 3BDO, the mTOR inducer. Western blot analysed the levels of EMT and autophagy associated proteins. Results: Administration of curcumin obviously reduced the levels of blood glucose, serum creatinine, urea nitrogen and urine albumen (by 28.4, 37.6, 33.5 and 22.4%, respectively), and attenuated renal histomorphological changes in DN rats. Podocytes were partially fused and autophagic vacuoles were increased in curcumin-treated rats. Furthermore, curcumin upregulated the expression of E-cadherin and LC3 proteins and downregulated the vimentin, TWIST1, p62, p-mTOR, p-Akt and P13K levels in DN rats and MPC5 cells. However, fumonisin B1 or 3BDO reversed the effects of curcumin on the expression of these proteins in cells. Discussion and conclusions: The protection against development of DN by curcumin treatment involved changes in inducing autophagy and alleviating podocyte EMT, through the PI3k/Akt/mTOR pathway, providing the scientific basis for further research and clinical applications of curcumin.Qiudi TuYiwen LiJuan JinXinxin JiangYan RenQiang HeTaylor & Francis Grouparticleglomerular podocytesepithelial-to-mesenchymal transitionp13k/aktmtorTherapeutics. PharmacologyRM1-950ENPharmaceutical Biology, Vol 57, Iss 1, Pp 778-786 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	glomerular podocytes epithelial-to-mesenchymal transition p13k/akt mtor Therapeutics. Pharmacology RM1-950
spellingShingle	glomerular podocytes epithelial-to-mesenchymal transition p13k/akt mtor Therapeutics. Pharmacology RM1-950 Qiudi Tu Yiwen Li Juan Jin Xinxin Jiang Yan Ren Qiang He Curcumin alleviates diabetic nephropathy via inhibiting podocyte mesenchymal transdifferentiation and inducing autophagy in rats and MPC5 cells
description	Context: Curcumin could ameliorate diabetic nephropathy (DN), but the mechanism remains unclear. Objective: The efficacy of curcumin on epithelial-to-mesenchymal transition (EMT) of podocyte and autophagy in vivo and in vitro was explored. Materials and methods: Thirty male Sprague–Dawley rats were divided into the normal, model and curcumin (300 mg/kg/d, i.g., for 8 weeks) groups. Rats received streptozotocin (50 mg/kg, i.p.) and high-fat-sugar diet to induce DN. Biochemical indicators and histomorphology of renal tissues were observed. In addition, cultured mouse podocytes (MPC5) was induced to EMT with serum from DN rats, and then exposed to curcumin (40 µM) with or without fumonisin B1, an Akt specific activator or 3BDO, the mTOR inducer. Western blot analysed the levels of EMT and autophagy associated proteins. Results: Administration of curcumin obviously reduced the levels of blood glucose, serum creatinine, urea nitrogen and urine albumen (by 28.4, 37.6, 33.5 and 22.4%, respectively), and attenuated renal histomorphological changes in DN rats. Podocytes were partially fused and autophagic vacuoles were increased in curcumin-treated rats. Furthermore, curcumin upregulated the expression of E-cadherin and LC3 proteins and downregulated the vimentin, TWIST1, p62, p-mTOR, p-Akt and P13K levels in DN rats and MPC5 cells. However, fumonisin B1 or 3BDO reversed the effects of curcumin on the expression of these proteins in cells. Discussion and conclusions: The protection against development of DN by curcumin treatment involved changes in inducing autophagy and alleviating podocyte EMT, through the PI3k/Akt/mTOR pathway, providing the scientific basis for further research and clinical applications of curcumin.
format	article
author	Qiudi Tu Yiwen Li Juan Jin Xinxin Jiang Yan Ren Qiang He
author_facet	Qiudi Tu Yiwen Li Juan Jin Xinxin Jiang Yan Ren Qiang He
author_sort	Qiudi Tu
title	Curcumin alleviates diabetic nephropathy via inhibiting podocyte mesenchymal transdifferentiation and inducing autophagy in rats and MPC5 cells
title_short	Curcumin alleviates diabetic nephropathy via inhibiting podocyte mesenchymal transdifferentiation and inducing autophagy in rats and MPC5 cells
title_full	Curcumin alleviates diabetic nephropathy via inhibiting podocyte mesenchymal transdifferentiation and inducing autophagy in rats and MPC5 cells
title_fullStr	Curcumin alleviates diabetic nephropathy via inhibiting podocyte mesenchymal transdifferentiation and inducing autophagy in rats and MPC5 cells
title_full_unstemmed	Curcumin alleviates diabetic nephropathy via inhibiting podocyte mesenchymal transdifferentiation and inducing autophagy in rats and MPC5 cells
title_sort	curcumin alleviates diabetic nephropathy via inhibiting podocyte mesenchymal transdifferentiation and inducing autophagy in rats and mpc5 cells
publisher	Taylor & Francis Group
publishDate	2019
url	https://doaj.org/article/9e212d5ebc144bfcb89067838fd87d8a
work_keys_str_mv	AT qiuditu curcuminalleviatesdiabeticnephropathyviainhibitingpodocytemesenchymaltransdifferentiationandinducingautophagyinratsandmpc5cells AT yiwenli curcuminalleviatesdiabeticnephropathyviainhibitingpodocytemesenchymaltransdifferentiationandinducingautophagyinratsandmpc5cells AT juanjin curcuminalleviatesdiabeticnephropathyviainhibitingpodocytemesenchymaltransdifferentiationandinducingautophagyinratsandmpc5cells AT xinxinjiang curcuminalleviatesdiabeticnephropathyviainhibitingpodocytemesenchymaltransdifferentiationandinducingautophagyinratsandmpc5cells AT yanren curcuminalleviatesdiabeticnephropathyviainhibitingpodocytemesenchymaltransdifferentiationandinducingautophagyinratsandmpc5cells AT qianghe curcuminalleviatesdiabeticnephropathyviainhibitingpodocytemesenchymaltransdifferentiationandinducingautophagyinratsandmpc5cells
_version_	1718425499043102720

Curcumin alleviates diabetic nephropathy via inhibiting podocyte mesenchymal transdifferentiation and inducing autophagy in rats and MPC5 cells

Ejemplares similares