Sensitivity and Specificity of P16 Immunohistochemistry in Diagnosis of Dysplastic Neoplastic Changes in Cervix
BACKGROUND AND OBJECTIVE: Cervical cancer is the second most common cancer in women that early diagnosis of its preneoplastic lesion can reduce cancer development. The aim of this study was to evaluate P16 role as a biomarker in diagnosis of dysplastic and neoplastic lesions of uterine cervix with i...
Guardado en:
| Autores principales: | , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN FA |
| Publicado: |
Babol University of Medical Sciences
2009
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/9e22be5ab2d947b58625f641dd05fc36 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| Sumario: | BACKGROUND AND OBJECTIVE: Cervical cancer is the second most common cancer in women that early diagnosis of its preneoplastic lesion can reduce cancer development. The aim of this study was to evaluate P16 role as a biomarker in diagnosis of dysplastic and neoplastic lesions of uterine cervix with immunohistochemistry.METHODS: This study was done on samples of 96 women in pathology department of Shahid Yahyanejad hospital, Babol, Iran. The samples divided into 4 groups (30 cases of normal cervix, 30 cases of cervical immature metaplasia, 30 cases of cervical dysplasia and 6 cases of cervical malignancy of squamous cell type). Immunohistochemical method was performed using kit of Dako company, made in Denmark, (p16INK4a, Clone E6H4), on paraffin embedded samples. P16 staining results, histopathological findings and age of women were analyzed and compared.FINDINGS: P16 staining was positive in 10% of normal cervix (3 subjects), 30% of cervical immature metaplasia (9 subjects), 63.3% of cervical dysplasia (19 subjects) and 83.3% of normal cervix (5 subjects). In cases with dysplasia P16 was positive in 4 subjects of grade I, 8 subjects of grade II and 7 subjects of grade III that there was not a significant relationship. The sensitivity of P16 was 83.3% in uterine cervical squamous cancer and sensitivity was respectively 63.3% in dysplasia and 30% in immature squamous metaplasia. The specificity of P16 was 80%.CONCLUSION: Result show that P16 had suitable sensitivity and specificity for diagnosis of dysplasia and neoplasia of uterine cervix and can be used in diagnosis of cancer lesions. |
|---|