Sensitivity and Specificity of P16 Immunohistochemistry in Diagnosis of Dysplastic Neoplastic Changes in Cervix

BACKGROUND AND OBJECTIVE: Cervical cancer is the second most common cancer in women that early diagnosis of its preneoplastic lesion can reduce cancer development. The aim of this study was to evaluate P16 role as a biomarker in diagnosis of dysplastic and neoplastic lesions of uterine cervix with i...

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Autores principales:	M Sharbatdaran, SH Shafaee, M Zeinalzadeh, H Ranjbar, B Jahed
Formato:	article
Lenguaje:	EN FA
Publicado:	Babol University of Medical Sciences 2009
Materias:	sensitivity specificity uterine cervical dysplasia metaplasia uterine cervical neoplasm cervical intraepithelial neoplasia cycline dependent kinase inhibitor p16 Medicine R Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/9e22be5ab2d947b58625f641dd05fc36
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spelling	oai:doaj.org-article:9e22be5ab2d947b58625f641dd05fc362021-11-10T09:02:34ZSensitivity and Specificity of P16 Immunohistochemistry in Diagnosis of Dysplastic Neoplastic Changes in Cervix1561-41072251-7170https://doaj.org/article/9e22be5ab2d947b58625f641dd05fc362009-04-01T00:00:00Zhttp://jbums.org/article-1-3145-en.htmlhttps://doaj.org/toc/1561-4107https://doaj.org/toc/2251-7170BACKGROUND AND OBJECTIVE: Cervical cancer is the second most common cancer in women that early diagnosis of its preneoplastic lesion can reduce cancer development. The aim of this study was to evaluate P16 role as a biomarker in diagnosis of dysplastic and neoplastic lesions of uterine cervix with immunohistochemistry.METHODS: This study was done on samples of 96 women in pathology department of Shahid Yahyanejad hospital, Babol, Iran. The samples divided into 4 groups (30 cases of normal cervix, 30 cases of cervical immature metaplasia, 30 cases of cervical dysplasia and 6 cases of cervical malignancy of squamous cell type). Immunohistochemical method was performed using kit of Dako company, made in Denmark, (p16INK4a, Clone E6H4), on paraffin embedded samples. P16 staining results, histopathological findings and age of women were analyzed and compared.FINDINGS: P16 staining was positive in 10% of normal cervix (3 subjects), 30% of cervical immature metaplasia (9 subjects), 63.3% of cervical dysplasia (19 subjects) and 83.3% of normal cervix (5 subjects). In cases with dysplasia P16 was positive in 4 subjects of grade I, 8 subjects of grade II and 7 subjects of grade III that there was not a significant relationship. The sensitivity of P16 was 83.3% in uterine cervical squamous cancer and sensitivity was respectively 63.3% in dysplasia and 30% in immature squamous metaplasia. The specificity of P16 was 80%.CONCLUSION: Result show that P16 had suitable sensitivity and specificity for diagnosis of dysplasia and neoplasia of uterine cervix and can be used in diagnosis of cancer lesions.M Sharbatdaran,SH ShafaeeM ZeinalzadehH RanjbarB JahedBabol University of Medical Sciencesarticlesensitivityspecificityuterine cervical dysplasiametaplasiauterine cervical neoplasmcervical intraepithelial neoplasiacycline dependent kinase inhibitor p16MedicineRMedicine (General)R5-920ENFAMajallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Bābul, Vol 11, Iss 1, Pp 8-13 (2009)
institution	DOAJ
collection	DOAJ
language	EN FA
topic	sensitivity specificity uterine cervical dysplasia metaplasia uterine cervical neoplasm cervical intraepithelial neoplasia cycline dependent kinase inhibitor p16 Medicine R Medicine (General) R5-920
spellingShingle	sensitivity specificity uterine cervical dysplasia metaplasia uterine cervical neoplasm cervical intraepithelial neoplasia cycline dependent kinase inhibitor p16 Medicine R Medicine (General) R5-920 M Sharbatdaran, SH Shafaee M Zeinalzadeh H Ranjbar B Jahed Sensitivity and Specificity of P16 Immunohistochemistry in Diagnosis of Dysplastic Neoplastic Changes in Cervix
description	BACKGROUND AND OBJECTIVE: Cervical cancer is the second most common cancer in women that early diagnosis of its preneoplastic lesion can reduce cancer development. The aim of this study was to evaluate P16 role as a biomarker in diagnosis of dysplastic and neoplastic lesions of uterine cervix with immunohistochemistry.METHODS: This study was done on samples of 96 women in pathology department of Shahid Yahyanejad hospital, Babol, Iran. The samples divided into 4 groups (30 cases of normal cervix, 30 cases of cervical immature metaplasia, 30 cases of cervical dysplasia and 6 cases of cervical malignancy of squamous cell type). Immunohistochemical method was performed using kit of Dako company, made in Denmark, (p16INK4a, Clone E6H4), on paraffin embedded samples. P16 staining results, histopathological findings and age of women were analyzed and compared.FINDINGS: P16 staining was positive in 10% of normal cervix (3 subjects), 30% of cervical immature metaplasia (9 subjects), 63.3% of cervical dysplasia (19 subjects) and 83.3% of normal cervix (5 subjects). In cases with dysplasia P16 was positive in 4 subjects of grade I, 8 subjects of grade II and 7 subjects of grade III that there was not a significant relationship. The sensitivity of P16 was 83.3% in uterine cervical squamous cancer and sensitivity was respectively 63.3% in dysplasia and 30% in immature squamous metaplasia. The specificity of P16 was 80%.CONCLUSION: Result show that P16 had suitable sensitivity and specificity for diagnosis of dysplasia and neoplasia of uterine cervix and can be used in diagnosis of cancer lesions.
format	article
author	M Sharbatdaran, SH Shafaee M Zeinalzadeh H Ranjbar B Jahed
author_facet	M Sharbatdaran, SH Shafaee M Zeinalzadeh H Ranjbar B Jahed
author_sort	M Sharbatdaran,
title	Sensitivity and Specificity of P16 Immunohistochemistry in Diagnosis of Dysplastic Neoplastic Changes in Cervix
title_short	Sensitivity and Specificity of P16 Immunohistochemistry in Diagnosis of Dysplastic Neoplastic Changes in Cervix
title_full	Sensitivity and Specificity of P16 Immunohistochemistry in Diagnosis of Dysplastic Neoplastic Changes in Cervix
title_fullStr	Sensitivity and Specificity of P16 Immunohistochemistry in Diagnosis of Dysplastic Neoplastic Changes in Cervix
title_full_unstemmed	Sensitivity and Specificity of P16 Immunohistochemistry in Diagnosis of Dysplastic Neoplastic Changes in Cervix
title_sort	sensitivity and specificity of p16 immunohistochemistry in diagnosis of dysplastic neoplastic changes in cervix
publisher	Babol University of Medical Sciences
publishDate	2009
url	https://doaj.org/article/9e22be5ab2d947b58625f641dd05fc36
work_keys_str_mv	AT msharbatdaran sensitivityandspecificityofp16immunohistochemistryindiagnosisofdysplasticneoplasticchangesincervix AT shshafaee sensitivityandspecificityofp16immunohistochemistryindiagnosisofdysplasticneoplasticchangesincervix AT mzeinalzadeh sensitivityandspecificityofp16immunohistochemistryindiagnosisofdysplasticneoplasticchangesincervix AT hranjbar sensitivityandspecificityofp16immunohistochemistryindiagnosisofdysplasticneoplasticchangesincervix AT bjahed sensitivityandspecificityofp16immunohistochemistryindiagnosisofdysplasticneoplasticchangesincervix
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Sensitivity and Specificity of P16 Immunohistochemistry in Diagnosis of Dysplastic Neoplastic Changes in Cervix

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