<i>RsSOS1</i> Responding to Salt Stress Might Be Involved in Regulating Salt Tolerance by Maintaining Na<sup>+</sup> Homeostasis in Radish (<i>Raphanus sativus</i> L.)
Radish is a kind of moderately salt-sensitive vegetable. Salt stress seriously decreases the yield and quality of radish. The plasma membrane Na<sup>+</sup>/H<sup>+</sup> antiporter protein Salt Overly Sensitive 1 (SOS1) plays a crucial role in protecting plant cells against...
Guardado en:
| Autores principales: | , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
MDPI AG
2021
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/9e3011a4b820455b828a37889e746f43 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| Sumario: | Radish is a kind of moderately salt-sensitive vegetable. Salt stress seriously decreases the yield and quality of radish. The plasma membrane Na<sup>+</sup>/H<sup>+</sup> antiporter protein Salt Overly Sensitive 1 (SOS1) plays a crucial role in protecting plant cells against salt stress, but the biological function of the <i>RsSOS1</i> gene in radish remains to be elucidated. In this study, the <i>RsSOS1</i> gene was isolated from radish genotype ‘NAU-TR17’, and contains an open reading frame of 3414 bp encoding 1137 amino acids. Phylogenetic analysis showed that RsSOS1 had a high homology with BnSOS1, and clustered together with <i>Arabidopsis</i> plasma membrane Na<sup>+</sup>/H<sup>+</sup> antiporter (AtNHX7). The result of subcellular localization indicated that the RsSOS1 was localized in the plasma membrane. Furthermore, <i>RsSOS1</i> was strongly induced in roots of radish under 150 mmol/L NaCl treatment, and its expression level in salt-tolerant genotypes was significantly higher than that in salt-sensitive ones. In addition, overexpression of <i>RsSOS1</i> in Arabidopsis could significantly improve the salt tolerance of transgenic plants. Meanwhile, the transformation of <i>RsSOS1</i><i>△999</i> could rescue Na<sup>+</sup> efflux function of AXT3 yeast. In summary, the plasma membrane Na<sup>+</sup>/H<sup>+</sup> antiporter RsSOS1 plays a vital role in regulating salt-tolerance of radish by controlling Na<sup>+</sup> homeostasis. These results provided useful information for further functional characterization of RsSOS1 and facilitate clarifying the molecular mechanism underlying salt stress response in radish. |
|---|