RsSOS1 Responding to Salt Stress Might Be Involved in Regulating Salt Tolerance by Maintaining Na+ Homeostasis in Radish (Raphanus sativus L.)

Radish is a kind of moderately salt-sensitive vegetable. Salt stress seriously decreases the yield and quality of radish. The plasma membrane Na+/H+ antiporter protein Salt Overly Sensitive 1 (SOS1) plays a crucial role in protecting plant cells against...

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Autores principales:	Wanting Zhang, Jingxue Li, Junhui Dong, Yan Wang, Liang Xu, Kexin Li, Xiaofang Yi, Yuelin Zhu, Liwang Liu
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	radish <i>RsSOS1</i> salt stress RT-qPCR overexpression yeast functional complement Plant culture SB1-1110
Acceso en línea:	https://doaj.org/article/9e3011a4b820455b828a37889e746f43
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spelling	oai:doaj.org-article:9e3011a4b820455b828a37889e746f432021-11-25T17:47:22Z<i>RsSOS1</i> Responding to Salt Stress Might Be Involved in Regulating Salt Tolerance by Maintaining Na<sup>+</sup> Homeostasis in Radish (<i>Raphanus sativus</i> L.)10.3390/horticulturae71104582311-7524https://doaj.org/article/9e3011a4b820455b828a37889e746f432021-11-01T00:00:00Zhttps://www.mdpi.com/2311-7524/7/11/458https://doaj.org/toc/2311-7524Radish is a kind of moderately salt-sensitive vegetable. Salt stress seriously decreases the yield and quality of radish. The plasma membrane Na<sup>+</sup>/H<sup>+</sup> antiporter protein Salt Overly Sensitive 1 (SOS1) plays a crucial role in protecting plant cells against salt stress, but the biological function of the <i>RsSOS1</i> gene in radish remains to be elucidated. In this study, the <i>RsSOS1</i> gene was isolated from radish genotype ‘NAU-TR17’, and contains an open reading frame of 3414 bp encoding 1137 amino acids. Phylogenetic analysis showed that RsSOS1 had a high homology with BnSOS1, and clustered together with <i>Arabidopsis</i> plasma membrane Na<sup>+</sup>/H<sup>+</sup> antiporter (AtNHX7). The result of subcellular localization indicated that the RsSOS1 was localized in the plasma membrane. Furthermore, <i>RsSOS1</i> was strongly induced in roots of radish under 150 mmol/L NaCl treatment, and its expression level in salt-tolerant genotypes was significantly higher than that in salt-sensitive ones. In addition, overexpression of <i>RsSOS1</i> in Arabidopsis could significantly improve the salt tolerance of transgenic plants. Meanwhile, the transformation of <i>RsSOS1</i><i>△999</i> could rescue Na<sup>+</sup> efflux function of AXT3 yeast. In summary, the plasma membrane Na<sup>+</sup>/H<sup>+</sup> antiporter RsSOS1 plays a vital role in regulating salt-tolerance of radish by controlling Na<sup>+</sup> homeostasis. These results provided useful information for further functional characterization of RsSOS1 and facilitate clarifying the molecular mechanism underlying salt stress response in radish.Wanting ZhangJingxue LiJunhui DongYan WangLiang XuKexin LiXiaofang YiYuelin ZhuLiwang LiuMDPI AGarticleradish<i>RsSOS1</i>salt stressRT-qPCRoverexpressionyeast functional complementPlant cultureSB1-1110ENHorticulturae, Vol 7, Iss 458, p 458 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	radish <i>RsSOS1</i> salt stress RT-qPCR overexpression yeast functional complement Plant culture SB1-1110
spellingShingle	radish <i>RsSOS1</i> salt stress RT-qPCR overexpression yeast functional complement Plant culture SB1-1110 Wanting Zhang Jingxue Li Junhui Dong Yan Wang Liang Xu Kexin Li Xiaofang Yi Yuelin Zhu Liwang Liu <i>RsSOS1</i> Responding to Salt Stress Might Be Involved in Regulating Salt Tolerance by Maintaining Na<sup>+</sup> Homeostasis in Radish (<i>Raphanus sativus</i> L.)
description	Radish is a kind of moderately salt-sensitive vegetable. Salt stress seriously decreases the yield and quality of radish. The plasma membrane Na<sup>+</sup>/H<sup>+</sup> antiporter protein Salt Overly Sensitive 1 (SOS1) plays a crucial role in protecting plant cells against salt stress, but the biological function of the <i>RsSOS1</i> gene in radish remains to be elucidated. In this study, the <i>RsSOS1</i> gene was isolated from radish genotype ‘NAU-TR17’, and contains an open reading frame of 3414 bp encoding 1137 amino acids. Phylogenetic analysis showed that RsSOS1 had a high homology with BnSOS1, and clustered together with <i>Arabidopsis</i> plasma membrane Na<sup>+</sup>/H<sup>+</sup> antiporter (AtNHX7). The result of subcellular localization indicated that the RsSOS1 was localized in the plasma membrane. Furthermore, <i>RsSOS1</i> was strongly induced in roots of radish under 150 mmol/L NaCl treatment, and its expression level in salt-tolerant genotypes was significantly higher than that in salt-sensitive ones. In addition, overexpression of <i>RsSOS1</i> in Arabidopsis could significantly improve the salt tolerance of transgenic plants. Meanwhile, the transformation of <i>RsSOS1</i><i>△999</i> could rescue Na<sup>+</sup> efflux function of AXT3 yeast. In summary, the plasma membrane Na<sup>+</sup>/H<sup>+</sup> antiporter RsSOS1 plays a vital role in regulating salt-tolerance of radish by controlling Na<sup>+</sup> homeostasis. These results provided useful information for further functional characterization of RsSOS1 and facilitate clarifying the molecular mechanism underlying salt stress response in radish.
format	article
author	Wanting Zhang Jingxue Li Junhui Dong Yan Wang Liang Xu Kexin Li Xiaofang Yi Yuelin Zhu Liwang Liu
author_facet	Wanting Zhang Jingxue Li Junhui Dong Yan Wang Liang Xu Kexin Li Xiaofang Yi Yuelin Zhu Liwang Liu
author_sort	Wanting Zhang
title	<i>RsSOS1</i> Responding to Salt Stress Might Be Involved in Regulating Salt Tolerance by Maintaining Na<sup>+</sup> Homeostasis in Radish (<i>Raphanus sativus</i> L.)
title_short	<i>RsSOS1</i> Responding to Salt Stress Might Be Involved in Regulating Salt Tolerance by Maintaining Na<sup>+</sup> Homeostasis in Radish (<i>Raphanus sativus</i> L.)
title_full	<i>RsSOS1</i> Responding to Salt Stress Might Be Involved in Regulating Salt Tolerance by Maintaining Na<sup>+</sup> Homeostasis in Radish (<i>Raphanus sativus</i> L.)
title_fullStr	<i>RsSOS1</i> Responding to Salt Stress Might Be Involved in Regulating Salt Tolerance by Maintaining Na<sup>+</sup> Homeostasis in Radish (<i>Raphanus sativus</i> L.)
title_full_unstemmed	<i>RsSOS1</i> Responding to Salt Stress Might Be Involved in Regulating Salt Tolerance by Maintaining Na<sup>+</sup> Homeostasis in Radish (<i>Raphanus sativus</i> L.)
title_sort	<i>rssos1</i> responding to salt stress might be involved in regulating salt tolerance by maintaining na<sup>+</sup> homeostasis in radish (<i>raphanus sativus</i> l.)
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/9e3011a4b820455b828a37889e746f43
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<i>RsSOS1</i> Responding to Salt Stress Might Be Involved in Regulating Salt Tolerance by Maintaining Na<sup>+</sup> Homeostasis in Radish (<i>Raphanus sativus</i> L.)

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