Performance characteristics and costs of serological tests for brucellosis in a pastoralist community of northern Tanzania

Performance characteristics and costs of serological tests for brucellosis in a pastoralist community of northern Tanzania

Abstract The control of brucellosis across sub-Saharan Africa is hampered by the lack of standardized testing and the use of tests with poor performance. This study evaluated the performance and costs of serological assays for human brucellosis in a pastoralist community in northern Tanzania. Serum...

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Autores principales: AbdulHamid S. Lukambagire, Ângelo J. Mendes, Rebecca F. Bodenham, John A. McGiven, Nestory A. Mkenda, Coletha Mathew, Matthew P. Rubach, Philoteus Sakasaka, Davis D. Shayo, Venance P. Maro, Gabriel M. Shirima, Kate M. Thomas, Christopher J. Kasanga, Rudovick R. Kazwala, Jo E. B. Halliday, Blandina T. Mmbaga
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Publicado: Nature Portfolio 2021
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Acceso en línea:https://doaj.org/article/9e312ed9f1d94f9fabe854e865644543
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spelling oai:doaj.org-article:9e312ed9f1d94f9fabe854e8656445432021-12-02T13:34:51ZPerformance characteristics and costs of serological tests for brucellosis in a pastoralist community of northern Tanzania10.1038/s41598-021-82906-w2045-2322https://doaj.org/article/9e312ed9f1d94f9fabe854e8656445432021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-82906-whttps://doaj.org/toc/2045-2322Abstract The control of brucellosis across sub-Saharan Africa is hampered by the lack of standardized testing and the use of tests with poor performance. This study evaluated the performance and costs of serological assays for human brucellosis in a pastoralist community in northern Tanzania. Serum collected from 218 febrile hospital patients was used to evaluate the performance of seven index tests, selected based on international recommendation or current use. We evaluated the Rose Bengal test (RBT) using two protocols, four commercial agglutination tests and a competitive enzyme-linked immunosorbent assay (cELISA). The sensitivity, specificity, positive predictive value, negative predictive value, Youden’s index, diagnostic accuracy, and per-sample cost of each index test were estimated. The diagnostic accuracy estimates ranged from 95.9 to 97.7% for the RBT, 55.0 to 72.0% for the commercial plate tests, and 89.4% for the cELISA. The per-sample cost range was $0.69–$0.79 for the RBT, $1.03–$1.14 for the commercial plate tests, and $2.51 for the cELISA. The widely used commercial plate tests performed poorly and cost more than the RBT. These findings provide evidence for the public health value of discontinuing the use of commercial agglutination tests for human brucellosis in Tanzania.AbdulHamid S. LukambagireÂngelo J. MendesRebecca F. BodenhamJohn A. McGivenNestory A. MkendaColetha MathewMatthew P. RubachPhiloteus SakasakaDavis D. ShayoVenance P. MaroGabriel M. ShirimaKate M. ThomasChristopher J. KasangaRudovick R. KazwalaJo E. B. HallidayBlandina T. MmbagaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
AbdulHamid S. Lukambagire
Ângelo J. Mendes
Rebecca F. Bodenham
John A. McGiven
Nestory A. Mkenda
Coletha Mathew
Matthew P. Rubach
Philoteus Sakasaka
Davis D. Shayo
Venance P. Maro
Gabriel M. Shirima
Kate M. Thomas
Christopher J. Kasanga
Rudovick R. Kazwala
Jo E. B. Halliday
Blandina T. Mmbaga
Performance characteristics and costs of serological tests for brucellosis in a pastoralist community of northern Tanzania
description Abstract The control of brucellosis across sub-Saharan Africa is hampered by the lack of standardized testing and the use of tests with poor performance. This study evaluated the performance and costs of serological assays for human brucellosis in a pastoralist community in northern Tanzania. Serum collected from 218 febrile hospital patients was used to evaluate the performance of seven index tests, selected based on international recommendation or current use. We evaluated the Rose Bengal test (RBT) using two protocols, four commercial agglutination tests and a competitive enzyme-linked immunosorbent assay (cELISA). The sensitivity, specificity, positive predictive value, negative predictive value, Youden’s index, diagnostic accuracy, and per-sample cost of each index test were estimated. The diagnostic accuracy estimates ranged from 95.9 to 97.7% for the RBT, 55.0 to 72.0% for the commercial plate tests, and 89.4% for the cELISA. The per-sample cost range was $0.69–$0.79 for the RBT, $1.03–$1.14 for the commercial plate tests, and $2.51 for the cELISA. The widely used commercial plate tests performed poorly and cost more than the RBT. These findings provide evidence for the public health value of discontinuing the use of commercial agglutination tests for human brucellosis in Tanzania.
format article
author AbdulHamid S. Lukambagire
Ângelo J. Mendes
Rebecca F. Bodenham
John A. McGiven
Nestory A. Mkenda
Coletha Mathew
Matthew P. Rubach
Philoteus Sakasaka
Davis D. Shayo
Venance P. Maro
Gabriel M. Shirima
Kate M. Thomas
Christopher J. Kasanga
Rudovick R. Kazwala
Jo E. B. Halliday
Blandina T. Mmbaga
author_facet AbdulHamid S. Lukambagire
Ângelo J. Mendes
Rebecca F. Bodenham
John A. McGiven
Nestory A. Mkenda
Coletha Mathew
Matthew P. Rubach
Philoteus Sakasaka
Davis D. Shayo
Venance P. Maro
Gabriel M. Shirima
Kate M. Thomas
Christopher J. Kasanga
Rudovick R. Kazwala
Jo E. B. Halliday
Blandina T. Mmbaga
author_sort AbdulHamid S. Lukambagire
title Performance characteristics and costs of serological tests for brucellosis in a pastoralist community of northern Tanzania
title_short Performance characteristics and costs of serological tests for brucellosis in a pastoralist community of northern Tanzania
title_full Performance characteristics and costs of serological tests for brucellosis in a pastoralist community of northern Tanzania
title_fullStr Performance characteristics and costs of serological tests for brucellosis in a pastoralist community of northern Tanzania
title_full_unstemmed Performance characteristics and costs of serological tests for brucellosis in a pastoralist community of northern Tanzania
title_sort performance characteristics and costs of serological tests for brucellosis in a pastoralist community of northern tanzania
publisher Nature Portfolio
publishDate 2021
url https://doaj.org/article/9e312ed9f1d94f9fabe854e865644543
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