The non-canonical Wnt/PKC pathway regulates mitochondrial dynamics through degradation of the arm-like domain-containing protein Alex3.

The regulation of mitochondrial dynamics is vital in complex cell types, such as neurons, that transport and localize mitochondria in high energy-demanding cell domains. The Armcx3 gene encodes a mitochondrial-targeted protein (Alex3) that contains several arm-like domains. In a previous study we sh...

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Autores principales:	Román Serrat, Guillermo López-Doménech, Serena Mirra, Martí Quevedo, Jordi Garcia-Fernàndez, Fausto Ulloa, Ferrán Burgaya, Eduardo Soriano
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9e3780b8f9ac47d199e80d9791b94e0d
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spelling	oai:doaj.org-article:9e3780b8f9ac47d199e80d9791b94e0d2021-11-18T07:39:04ZThe non-canonical Wnt/PKC pathway regulates mitochondrial dynamics through degradation of the arm-like domain-containing protein Alex3.1932-620310.1371/journal.pone.0067773https://doaj.org/article/9e3780b8f9ac47d199e80d9791b94e0d2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23844091/?tool=EBIhttps://doaj.org/toc/1932-6203The regulation of mitochondrial dynamics is vital in complex cell types, such as neurons, that transport and localize mitochondria in high energy-demanding cell domains. The Armcx3 gene encodes a mitochondrial-targeted protein (Alex3) that contains several arm-like domains. In a previous study we showed that Alex3 protein regulates mitochondrial aggregation and trafficking. Here we studied the contribution of Wnt proteins to the mitochondrial aggregation and dynamics regulated by Alex3. Overexpression of Alex3 in HEK293 cells caused a marked aggregation of mitochondria, which was attenuated by treatment with several Wnts. We also found that this decrease was caused by Alex3 degradation induced by Wnts. While the Wnt canonical pathway did not alter the pattern of mitochondrial aggregation induced by Alex3, we observed that the Wnt/PKC non-canonical pathway regulated both mitochondrial aggregation and Alex3 protein levels, thereby rendering a mitochondrial phenotype and distribution similar to control patterns. Our data suggest that the Wnt pathway regulates mitochondrial distribution and dynamics through Alex3 protein degradation.Román SerratGuillermo López-DoménechSerena MirraMartí QuevedoJordi Garcia-FernàndezFausto UlloaFerrán BurgayaEduardo SorianoPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 7, p e67773 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Román Serrat Guillermo López-Doménech Serena Mirra Martí Quevedo Jordi Garcia-Fernàndez Fausto Ulloa Ferrán Burgaya Eduardo Soriano The non-canonical Wnt/PKC pathway regulates mitochondrial dynamics through degradation of the arm-like domain-containing protein Alex3.
description	The regulation of mitochondrial dynamics is vital in complex cell types, such as neurons, that transport and localize mitochondria in high energy-demanding cell domains. The Armcx3 gene encodes a mitochondrial-targeted protein (Alex3) that contains several arm-like domains. In a previous study we showed that Alex3 protein regulates mitochondrial aggregation and trafficking. Here we studied the contribution of Wnt proteins to the mitochondrial aggregation and dynamics regulated by Alex3. Overexpression of Alex3 in HEK293 cells caused a marked aggregation of mitochondria, which was attenuated by treatment with several Wnts. We also found that this decrease was caused by Alex3 degradation induced by Wnts. While the Wnt canonical pathway did not alter the pattern of mitochondrial aggregation induced by Alex3, we observed that the Wnt/PKC non-canonical pathway regulated both mitochondrial aggregation and Alex3 protein levels, thereby rendering a mitochondrial phenotype and distribution similar to control patterns. Our data suggest that the Wnt pathway regulates mitochondrial distribution and dynamics through Alex3 protein degradation.
format	article
author	Román Serrat Guillermo López-Doménech Serena Mirra Martí Quevedo Jordi Garcia-Fernàndez Fausto Ulloa Ferrán Burgaya Eduardo Soriano
author_facet	Román Serrat Guillermo López-Doménech Serena Mirra Martí Quevedo Jordi Garcia-Fernàndez Fausto Ulloa Ferrán Burgaya Eduardo Soriano
author_sort	Román Serrat
title	The non-canonical Wnt/PKC pathway regulates mitochondrial dynamics through degradation of the arm-like domain-containing protein Alex3.
title_short	The non-canonical Wnt/PKC pathway regulates mitochondrial dynamics through degradation of the arm-like domain-containing protein Alex3.
title_full	The non-canonical Wnt/PKC pathway regulates mitochondrial dynamics through degradation of the arm-like domain-containing protein Alex3.
title_fullStr	The non-canonical Wnt/PKC pathway regulates mitochondrial dynamics through degradation of the arm-like domain-containing protein Alex3.
title_full_unstemmed	The non-canonical Wnt/PKC pathway regulates mitochondrial dynamics through degradation of the arm-like domain-containing protein Alex3.
title_sort	non-canonical wnt/pkc pathway regulates mitochondrial dynamics through degradation of the arm-like domain-containing protein alex3.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/9e3780b8f9ac47d199e80d9791b94e0d
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The non-canonical Wnt/PKC pathway regulates mitochondrial dynamics through degradation of the arm-like domain-containing protein Alex3.

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