Axonal regeneration after sciatic nerve lesion is delayed but complete in GFAP- and vimentin-deficient mice.

Peripheral axotomy of motoneurons triggers Wallerian degeneration of injured axons distal to the lesion, followed by axon regeneration. Centrally, axotomy induces loss of synapses (synaptic stripping) from the surface of lesioned motoneurons in the spinal cord. At the lesion site, reactive Schwann c...

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Autores principales:	Alexander Berg, Johan Zelano, Marcela Pekna, Ulrika Wilhelmsson, Milos Pekny, Staffan Cullheim
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9e4368efa2ac454e93727fafd3d8bd7a
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spelling	oai:doaj.org-article:9e4368efa2ac454e93727fafd3d8bd7a2021-11-18T08:48:40ZAxonal regeneration after sciatic nerve lesion is delayed but complete in GFAP- and vimentin-deficient mice.1932-620310.1371/journal.pone.0079395https://doaj.org/article/9e4368efa2ac454e93727fafd3d8bd7a2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24223940/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Peripheral axotomy of motoneurons triggers Wallerian degeneration of injured axons distal to the lesion, followed by axon regeneration. Centrally, axotomy induces loss of synapses (synaptic stripping) from the surface of lesioned motoneurons in the spinal cord. At the lesion site, reactive Schwann cells provide trophic support and guidance for outgrowing axons. The mechanisms of synaptic stripping remain elusive, but reactive astrocytes and microglia appear to be important in this process. We studied axonal regeneration and synaptic stripping of motoneurons after a sciatic nerve lesion in mice lacking the intermediate filament (nanofilament) proteins glial fibrillary acidic protein (GFAP) and vimentin, which are upregulated in reactive astrocytes and Schwann cells. Seven days after sciatic nerve transection, ultrastructural analysis of synaptic density on the somata of injured motoneurons revealed more remaining boutons covering injured somata in GFAP(-/-)Vim(-/-) mice. After sciatic nerve crush in GFAP(-/-)Vim(-/-) mice, the fraction of reinnervated motor endplates on muscle fibers of the gastrocnemius muscle was reduced 13 days after the injury, and axonal regeneration and functional recovery were delayed but complete. Thus, the absence of GFAP and vimentin in glial cells does not seem to affect the outcome after peripheral motoneuron injury but may have an important effect on the response dynamics.Alexander BergJohan ZelanoMarcela PeknaUlrika WilhelmssonMilos PeknyStaffan CullheimPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 11, p e79395 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Alexander Berg Johan Zelano Marcela Pekna Ulrika Wilhelmsson Milos Pekny Staffan Cullheim Axonal regeneration after sciatic nerve lesion is delayed but complete in GFAP- and vimentin-deficient mice.
description	Peripheral axotomy of motoneurons triggers Wallerian degeneration of injured axons distal to the lesion, followed by axon regeneration. Centrally, axotomy induces loss of synapses (synaptic stripping) from the surface of lesioned motoneurons in the spinal cord. At the lesion site, reactive Schwann cells provide trophic support and guidance for outgrowing axons. The mechanisms of synaptic stripping remain elusive, but reactive astrocytes and microglia appear to be important in this process. We studied axonal regeneration and synaptic stripping of motoneurons after a sciatic nerve lesion in mice lacking the intermediate filament (nanofilament) proteins glial fibrillary acidic protein (GFAP) and vimentin, which are upregulated in reactive astrocytes and Schwann cells. Seven days after sciatic nerve transection, ultrastructural analysis of synaptic density on the somata of injured motoneurons revealed more remaining boutons covering injured somata in GFAP(-/-)Vim(-/-) mice. After sciatic nerve crush in GFAP(-/-)Vim(-/-) mice, the fraction of reinnervated motor endplates on muscle fibers of the gastrocnemius muscle was reduced 13 days after the injury, and axonal regeneration and functional recovery were delayed but complete. Thus, the absence of GFAP and vimentin in glial cells does not seem to affect the outcome after peripheral motoneuron injury but may have an important effect on the response dynamics.
format	article
author	Alexander Berg Johan Zelano Marcela Pekna Ulrika Wilhelmsson Milos Pekny Staffan Cullheim
author_facet	Alexander Berg Johan Zelano Marcela Pekna Ulrika Wilhelmsson Milos Pekny Staffan Cullheim
author_sort	Alexander Berg
title	Axonal regeneration after sciatic nerve lesion is delayed but complete in GFAP- and vimentin-deficient mice.
title_short	Axonal regeneration after sciatic nerve lesion is delayed but complete in GFAP- and vimentin-deficient mice.
title_full	Axonal regeneration after sciatic nerve lesion is delayed but complete in GFAP- and vimentin-deficient mice.
title_fullStr	Axonal regeneration after sciatic nerve lesion is delayed but complete in GFAP- and vimentin-deficient mice.
title_full_unstemmed	Axonal regeneration after sciatic nerve lesion is delayed but complete in GFAP- and vimentin-deficient mice.
title_sort	axonal regeneration after sciatic nerve lesion is delayed but complete in gfap- and vimentin-deficient mice.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/9e4368efa2ac454e93727fafd3d8bd7a
work_keys_str_mv	AT alexanderberg axonalregenerationaftersciaticnervelesionisdelayedbutcompleteingfapandvimentindeficientmice AT johanzelano axonalregenerationaftersciaticnervelesionisdelayedbutcompleteingfapandvimentindeficientmice AT marcelapekna axonalregenerationaftersciaticnervelesionisdelayedbutcompleteingfapandvimentindeficientmice AT ulrikawilhelmsson axonalregenerationaftersciaticnervelesionisdelayedbutcompleteingfapandvimentindeficientmice AT milospekny axonalregenerationaftersciaticnervelesionisdelayedbutcompleteingfapandvimentindeficientmice AT staffancullheim axonalregenerationaftersciaticnervelesionisdelayedbutcompleteingfapandvimentindeficientmice
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Axonal regeneration after sciatic nerve lesion is delayed but complete in GFAP- and vimentin-deficient mice.

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