EXTRACELLULAR MATRIX CONDITION IN CASE OF HCVASSOCIATED LIVER FIBROSIS

Imbalance of the proteolysis/antiproteolysis system is known to be among key components of immunofibrogenesis of liver in cases of chronic hepatitis C. To evaluate these aspects, we studied several factors of liver tissue remodeling in blood serum and local samples from HCV patients associated with...

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Autores principales:	I. S. Gorelova, L. F. Sklyar, E. V. Markelova, A. I. Simakova, I. V. Zenin
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2017
Materias:	matrix metalloproteinase tissue inhibitor of matrix metalloproteinase chronic hepatitis c liver fibrosis Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/9e515edd2f23400d82078c0fd5350737
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spelling	oai:doaj.org-article:9e515edd2f23400d82078c0fd53507372021-11-18T08:03:45ZEXTRACELLULAR MATRIX CONDITION IN CASE OF HCVASSOCIATED LIVER FIBROSIS1563-06252313-741X10.15789/1563-0625-2017-1-35-44https://doaj.org/article/9e515edd2f23400d82078c0fd53507372017-01-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/1163https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XImbalance of the proteolysis/antiproteolysis system is known to be among key components of immunofibrogenesis of liver in cases of chronic hepatitis C. To evaluate these aspects, we studied several factors of liver tissue remodeling in blood serum and local samples from HCV patients associated with liver fibrosis. We determined the levels of matrix metalloproteinase-9 (MMP-9), tissue inhibitor of matrix metalloproteinase-1 (TIMP-1), MMP-9/TIMP-1 and MMP-9/TIMP-2 complexes. Clinical, laboratory and instrumental examinations have been made for 81 patients with chronic hepatitis C who did not receive antiviral therapy, and 22 healthy volunteers. Extracellular matrix protein (ECM) profile was studied in 103 serum blood samples and 32 liver supernates using ELISA technique. Statistically significant increase of MMP-9 contents (p < 0.05) and its complexes with TIMP-1 (p < 0.05) and TIMP-2 (p < 0.01), as well as low levels of type 1 inhibitor (p < 0.05) were revealed in blood serum of HCV-infected patients, as compared with control group. Protein assays in liver supernates of hepatitis C patients reflecting extracellular matrix state revealed an eight-fold increase in MMP-9/ TIMP-1 complex, as compared with control group (p < 0.05). The values of other proteolytic/antiproteolytic factors proved to be low (p < 0.05). An imbalance in protease contents in blood serum and liver biopsies was revealed, showing differently directed changes. I.e., serum values of MMP-9, TIMP-1 and MMP-9/TIMP-2 during transition of liver fibrosis to cirrhosis (F0 to F4) became decreased (p < 0.05), associated with increased liver concentrations of these proteolytic enzymes (p < 0.05). In summary, we conclude that the data obtained in our study suggest an imbalance of proteolysis/antiproteolysis system leads to a dysregulated liver tissue remodeling in patients with chronic hepatitis C.I. S. GorelovaL. F. SklyarE. V. MarkelovaA. I. SimakovaI. V. ZeninSPb RAACIarticlematrix metalloproteinasetissue inhibitor of matrix metalloproteinasechronic hepatitis cliver fibrosisImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 19, Iss 1, Pp 35-44 (2017)
institution	DOAJ
collection	DOAJ
language	RU
topic	matrix metalloproteinase tissue inhibitor of matrix metalloproteinase chronic hepatitis c liver fibrosis Immunologic diseases. Allergy RC581-607
spellingShingle	matrix metalloproteinase tissue inhibitor of matrix metalloproteinase chronic hepatitis c liver fibrosis Immunologic diseases. Allergy RC581-607 I. S. Gorelova L. F. Sklyar E. V. Markelova A. I. Simakova I. V. Zenin EXTRACELLULAR MATRIX CONDITION IN CASE OF HCVASSOCIATED LIVER FIBROSIS
description	Imbalance of the proteolysis/antiproteolysis system is known to be among key components of immunofibrogenesis of liver in cases of chronic hepatitis C. To evaluate these aspects, we studied several factors of liver tissue remodeling in blood serum and local samples from HCV patients associated with liver fibrosis. We determined the levels of matrix metalloproteinase-9 (MMP-9), tissue inhibitor of matrix metalloproteinase-1 (TIMP-1), MMP-9/TIMP-1 and MMP-9/TIMP-2 complexes. Clinical, laboratory and instrumental examinations have been made for 81 patients with chronic hepatitis C who did not receive antiviral therapy, and 22 healthy volunteers. Extracellular matrix protein (ECM) profile was studied in 103 serum blood samples and 32 liver supernates using ELISA technique. Statistically significant increase of MMP-9 contents (p < 0.05) and its complexes with TIMP-1 (p < 0.05) and TIMP-2 (p < 0.01), as well as low levels of type 1 inhibitor (p < 0.05) were revealed in blood serum of HCV-infected patients, as compared with control group. Protein assays in liver supernates of hepatitis C patients reflecting extracellular matrix state revealed an eight-fold increase in MMP-9/ TIMP-1 complex, as compared with control group (p < 0.05). The values of other proteolytic/antiproteolytic factors proved to be low (p < 0.05). An imbalance in protease contents in blood serum and liver biopsies was revealed, showing differently directed changes. I.e., serum values of MMP-9, TIMP-1 and MMP-9/TIMP-2 during transition of liver fibrosis to cirrhosis (F0 to F4) became decreased (p < 0.05), associated with increased liver concentrations of these proteolytic enzymes (p < 0.05). In summary, we conclude that the data obtained in our study suggest an imbalance of proteolysis/antiproteolysis system leads to a dysregulated liver tissue remodeling in patients with chronic hepatitis C.
format	article
author	I. S. Gorelova L. F. Sklyar E. V. Markelova A. I. Simakova I. V. Zenin
author_facet	I. S. Gorelova L. F. Sklyar E. V. Markelova A. I. Simakova I. V. Zenin
author_sort	I. S. Gorelova
title	EXTRACELLULAR MATRIX CONDITION IN CASE OF HCVASSOCIATED LIVER FIBROSIS
title_short	EXTRACELLULAR MATRIX CONDITION IN CASE OF HCVASSOCIATED LIVER FIBROSIS
title_full	EXTRACELLULAR MATRIX CONDITION IN CASE OF HCVASSOCIATED LIVER FIBROSIS
title_fullStr	EXTRACELLULAR MATRIX CONDITION IN CASE OF HCVASSOCIATED LIVER FIBROSIS
title_full_unstemmed	EXTRACELLULAR MATRIX CONDITION IN CASE OF HCVASSOCIATED LIVER FIBROSIS
title_sort	extracellular matrix condition in case of hcvassociated liver fibrosis
publisher	SPb RAACI
publishDate	2017
url	https://doaj.org/article/9e515edd2f23400d82078c0fd5350737
work_keys_str_mv	AT isgorelova extracellularmatrixconditionincaseofhcvassociatedliverfibrosis AT lfsklyar extracellularmatrixconditionincaseofhcvassociatedliverfibrosis AT evmarkelova extracellularmatrixconditionincaseofhcvassociatedliverfibrosis AT aisimakova extracellularmatrixconditionincaseofhcvassociatedliverfibrosis AT ivzenin extracellularmatrixconditionincaseofhcvassociatedliverfibrosis
_version_	1718422437570281472

EXTRACELLULAR MATRIX CONDITION IN CASE OF HCVASSOCIATED LIVER FIBROSIS

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