GFAT and PFK genes show contrasting regulation of chitin metabolism in Nilaparvata lugens

Abstract Glutamine:fructose-6-phosphate aminotransferase (GFAT) and phosphofructokinase (PFK) are enzymes related to chitin metabolism. RNA interference (RNAi) technology was used to explore the role of these two enzyme genes in chitin metabolism. In this study, we found that GFAT and PFK were highl...

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Autores principales:	Cai-Di Xu, Yong-Kang Liu, Ling-Yu Qiu, Sha-Sha Wang, Bi-Ying Pan, Yan Li, Shi-Gui Wang, Bin Tang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9e51f66b12ce40c0ba685bec0eb2264e
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spelling	oai:doaj.org-article:9e51f66b12ce40c0ba685bec0eb2264e2021-12-02T13:33:44ZGFAT and PFK genes show contrasting regulation of chitin metabolism in Nilaparvata lugens10.1038/s41598-021-84760-22045-2322https://doaj.org/article/9e51f66b12ce40c0ba685bec0eb2264e2021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-84760-2https://doaj.org/toc/2045-2322Abstract Glutamine:fructose-6-phosphate aminotransferase (GFAT) and phosphofructokinase (PFK) are enzymes related to chitin metabolism. RNA interference (RNAi) technology was used to explore the role of these two enzyme genes in chitin metabolism. In this study, we found that GFAT and PFK were highly expressed in the wing bud of Nilaparvata lugens and were increased significantly during molting. RNAi of GFAT and PFK both caused severe malformation rates and mortality rates in N. lugens. GFAT inhibition also downregulated GFAT, GNPNA, PGM1, PGM2, UAP, CHS1, CHS1a, CHS1b, Cht1-10, and ENGase. PFK inhibition significantly downregulated GFAT; upregulated GNPNA, PGM2, UAP, Cht2-4, Cht6-7 at 48 h and then downregulated them at 72 h; upregulated Cht5, Cht8, Cht10, and ENGase; downregulated Cht9 at 48 h and then upregulated it at 72 h; and upregulated CHS1, CHS1a, and CHS1b. In conclusion, GFAT and PFK regulated chitin degradation and remodeling by regulating the expression of genes related to the chitin metabolism and exert opposite effects on these genes. These results may be beneficial to develop new chitin synthesis inhibitors for pest control.Cai-Di XuYong-Kang LiuLing-Yu QiuSha-Sha WangBi-Ying PanYan LiShi-Gui WangBin TangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Cai-Di Xu Yong-Kang Liu Ling-Yu Qiu Sha-Sha Wang Bi-Ying Pan Yan Li Shi-Gui Wang Bin Tang GFAT and PFK genes show contrasting regulation of chitin metabolism in Nilaparvata lugens
description	Abstract Glutamine:fructose-6-phosphate aminotransferase (GFAT) and phosphofructokinase (PFK) are enzymes related to chitin metabolism. RNA interference (RNAi) technology was used to explore the role of these two enzyme genes in chitin metabolism. In this study, we found that GFAT and PFK were highly expressed in the wing bud of Nilaparvata lugens and were increased significantly during molting. RNAi of GFAT and PFK both caused severe malformation rates and mortality rates in N. lugens. GFAT inhibition also downregulated GFAT, GNPNA, PGM1, PGM2, UAP, CHS1, CHS1a, CHS1b, Cht1-10, and ENGase. PFK inhibition significantly downregulated GFAT; upregulated GNPNA, PGM2, UAP, Cht2-4, Cht6-7 at 48 h and then downregulated them at 72 h; upregulated Cht5, Cht8, Cht10, and ENGase; downregulated Cht9 at 48 h and then upregulated it at 72 h; and upregulated CHS1, CHS1a, and CHS1b. In conclusion, GFAT and PFK regulated chitin degradation and remodeling by regulating the expression of genes related to the chitin metabolism and exert opposite effects on these genes. These results may be beneficial to develop new chitin synthesis inhibitors for pest control.
format	article
author	Cai-Di Xu Yong-Kang Liu Ling-Yu Qiu Sha-Sha Wang Bi-Ying Pan Yan Li Shi-Gui Wang Bin Tang
author_facet	Cai-Di Xu Yong-Kang Liu Ling-Yu Qiu Sha-Sha Wang Bi-Ying Pan Yan Li Shi-Gui Wang Bin Tang
author_sort	Cai-Di Xu
title	GFAT and PFK genes show contrasting regulation of chitin metabolism in Nilaparvata lugens
title_short	GFAT and PFK genes show contrasting regulation of chitin metabolism in Nilaparvata lugens
title_full	GFAT and PFK genes show contrasting regulation of chitin metabolism in Nilaparvata lugens
title_fullStr	GFAT and PFK genes show contrasting regulation of chitin metabolism in Nilaparvata lugens
title_full_unstemmed	GFAT and PFK genes show contrasting regulation of chitin metabolism in Nilaparvata lugens
title_sort	gfat and pfk genes show contrasting regulation of chitin metabolism in nilaparvata lugens
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/9e51f66b12ce40c0ba685bec0eb2264e
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GFAT and PFK genes show contrasting regulation of chitin metabolism in Nilaparvata lugens

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