DeSiphering receptor core-induced and ligand-dependent conformational changes in arrestin via genetic encoded trimethylsilyl 1H-NMR probe

DeSiphering receptor core-induced and ligand-dependent conformational changes in arrestin via genetic encoded trimethylsilyl 1H-NMR probe

Characterization of dynamic conformational changes in membrane protein complexes by NMR spectroscopy remains challenging. Here authors report the site-specific incorporation of 4-trimethylsilyl phenylalanine (TMSiPhe) into proteins, which enabled the characterization of multiple conformational state...

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Autores principales: Qi Liu, Qing-tao He, Xiaoxuan Lyu, Fan Yang, Zhong-liang Zhu, Peng Xiao, Zhao Yang, Feng Zhang, Zhao-ya Yang, Xiao-yan Wang, Peng Sun, Qian-wen Wang, Chang-xiu Qu, Zheng Gong, Jing-yu Lin, Zhen Xu, Shao-le Song, Shen-ming Huang, Sheng-chao Guo, Ming-jie Han, Kong-kai Zhu, Xin Chen, Alem W. Kahsai, Kun-Hong Xiao, Wei Kong, Fa-hui Li, Ke Ruan, Zi-jian Li, Xiao Yu, Xiao-gang Niu, Chang-wen Jin, Jiangyun Wang, Jin-peng Sun
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2020
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Acceso en línea:https://doaj.org/article/9e525d39c3704658bb31c1f7b6676d76
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spelling oai:doaj.org-article:9e525d39c3704658bb31c1f7b6676d762021-12-02T18:48:04ZDeSiphering receptor core-induced and ligand-dependent conformational changes in arrestin via genetic encoded trimethylsilyl 1H-NMR probe10.1038/s41467-020-18433-52041-1723https://doaj.org/article/9e525d39c3704658bb31c1f7b6676d762020-09-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-18433-5https://doaj.org/toc/2041-1723Characterization of dynamic conformational changes in membrane protein complexes by NMR spectroscopy remains challenging. Here authors report the site-specific incorporation of 4-trimethylsilyl phenylalanine (TMSiPhe) into proteins, which enabled the characterization of multiple conformational states of a phospho-β2 adrenergic receptor/β-arrestin-1 complex in response to different receptor ligands.Qi LiuQing-tao HeXiaoxuan LyuFan YangZhong-liang ZhuPeng XiaoZhao YangFeng ZhangZhao-ya YangXiao-yan WangPeng SunQian-wen WangChang-xiu QuZheng GongJing-yu LinZhen XuShao-le SongShen-ming HuangSheng-chao GuoMing-jie HanKong-kai ZhuXin ChenAlem W. KahsaiKun-Hong XiaoWei KongFa-hui LiKe RuanZi-jian LiXiao YuXiao-gang NiuChang-wen JinJiangyun WangJin-peng SunNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-17 (2020)
institution DOAJ
collection DOAJ
language EN
topic Science
Q
spellingShingle Science
Q
Qi Liu
Qing-tao He
Xiaoxuan Lyu
Fan Yang
Zhong-liang Zhu
Peng Xiao
Zhao Yang
Feng Zhang
Zhao-ya Yang
Xiao-yan Wang
Peng Sun
Qian-wen Wang
Chang-xiu Qu
Zheng Gong
Jing-yu Lin
Zhen Xu
Shao-le Song
Shen-ming Huang
Sheng-chao Guo
Ming-jie Han
Kong-kai Zhu
Xin Chen
Alem W. Kahsai
Kun-Hong Xiao
Wei Kong
Fa-hui Li
Ke Ruan
Zi-jian Li
Xiao Yu
Xiao-gang Niu
Chang-wen Jin
Jiangyun Wang
Jin-peng Sun
DeSiphering receptor core-induced and ligand-dependent conformational changes in arrestin via genetic encoded trimethylsilyl 1H-NMR probe
description Characterization of dynamic conformational changes in membrane protein complexes by NMR spectroscopy remains challenging. Here authors report the site-specific incorporation of 4-trimethylsilyl phenylalanine (TMSiPhe) into proteins, which enabled the characterization of multiple conformational states of a phospho-β2 adrenergic receptor/β-arrestin-1 complex in response to different receptor ligands.
format article
author Qi Liu
Qing-tao He
Xiaoxuan Lyu
Fan Yang
Zhong-liang Zhu
Peng Xiao
Zhao Yang
Feng Zhang
Zhao-ya Yang
Xiao-yan Wang
Peng Sun
Qian-wen Wang
Chang-xiu Qu
Zheng Gong
Jing-yu Lin
Zhen Xu
Shao-le Song
Shen-ming Huang
Sheng-chao Guo
Ming-jie Han
Kong-kai Zhu
Xin Chen
Alem W. Kahsai
Kun-Hong Xiao
Wei Kong
Fa-hui Li
Ke Ruan
Zi-jian Li
Xiao Yu
Xiao-gang Niu
Chang-wen Jin
Jiangyun Wang
Jin-peng Sun
author_facet Qi Liu
Qing-tao He
Xiaoxuan Lyu
Fan Yang
Zhong-liang Zhu
Peng Xiao
Zhao Yang
Feng Zhang
Zhao-ya Yang
Xiao-yan Wang
Peng Sun
Qian-wen Wang
Chang-xiu Qu
Zheng Gong
Jing-yu Lin
Zhen Xu
Shao-le Song
Shen-ming Huang
Sheng-chao Guo
Ming-jie Han
Kong-kai Zhu
Xin Chen
Alem W. Kahsai
Kun-Hong Xiao
Wei Kong
Fa-hui Li
Ke Ruan
Zi-jian Li
Xiao Yu
Xiao-gang Niu
Chang-wen Jin
Jiangyun Wang
Jin-peng Sun
author_sort Qi Liu
title DeSiphering receptor core-induced and ligand-dependent conformational changes in arrestin via genetic encoded trimethylsilyl 1H-NMR probe
title_short DeSiphering receptor core-induced and ligand-dependent conformational changes in arrestin via genetic encoded trimethylsilyl 1H-NMR probe
title_full DeSiphering receptor core-induced and ligand-dependent conformational changes in arrestin via genetic encoded trimethylsilyl 1H-NMR probe
title_fullStr DeSiphering receptor core-induced and ligand-dependent conformational changes in arrestin via genetic encoded trimethylsilyl 1H-NMR probe
title_full_unstemmed DeSiphering receptor core-induced and ligand-dependent conformational changes in arrestin via genetic encoded trimethylsilyl 1H-NMR probe
title_sort desiphering receptor core-induced and ligand-dependent conformational changes in arrestin via genetic encoded trimethylsilyl 1h-nmr probe
publisher Nature Portfolio
publishDate 2020
url https://doaj.org/article/9e525d39c3704658bb31c1f7b6676d76
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