Extracellular HSP70/HSP70-PCs promote epithelial-mesenchymal transition of hepatocarcinoma cells.

<h4>Background</h4>Extracellular heat shock protein 70 and peptide complexes (eHSP70/HSP70-PCs) regulate a variety of biological behaviors in tumor cells. Whether eHSP70/HSP70-PCs are involved in the epithelial-mesenchymal transition (EMT) of tumor cells remains unclear.<h4>Aims<...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Hangyu Li, Yan Li, Dan Liu, Hongzhi Sun, Dongming Su, Fuquan Yang, Jingang Liu
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9e55105e62d84a3f8baeafeb88da8ebb
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:9e55105e62d84a3f8baeafeb88da8ebb
record_format	dspace
spelling	oai:doaj.org-article:9e55105e62d84a3f8baeafeb88da8ebb2021-11-18T08:40:02ZExtracellular HSP70/HSP70-PCs promote epithelial-mesenchymal transition of hepatocarcinoma cells.1932-620310.1371/journal.pone.0084759https://doaj.org/article/9e55105e62d84a3f8baeafeb88da8ebb2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24386414/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Extracellular heat shock protein 70 and peptide complexes (eHSP70/HSP70-PCs) regulate a variety of biological behaviors in tumor cells. Whether eHSP70/HSP70-PCs are involved in the epithelial-mesenchymal transition (EMT) of tumor cells remains unclear.<h4>Aims</h4>To determine the effects of eHSP70/HSP70-PCs on EMT of hepatocarcinoma cells.<h4>Methods</h4>The expressions of E-cadherin, HSP70, α-smooth muscle actin protein (α-SMA) and p-p38 were detected immunohistochemically in liver cancer samples. Immunofluorescence, western blotting and real-time RT-PCR methods were used to analyze the effects of eHSP70/HSP70-PCs on the expressions of E-cadherin, α-SMA and p38/MAPK in vivo.<h4>Results</h4>HSP70, E-cadherin, α-SMA and p-p38 were elevated in hepatocellular carcinoma tissues. The expression of HSP70 was positively correlated with malignant differentiated liver carcinoma. The expressions of HSP70, α-SMA and p-p38 correlated with recurrence-free survival after resection. eHSP70/HSP70-PCs significantly promoted the expressions of α-SMA and p-p38 and reduced the expressions of E-cadherin in vivo. The effect was inhibited by SB203580.<h4>Conclusion</h4>The expressions of HSP70, E-cadherin, α-SMA and p-p38 may represent indicators of malignant potential and could discriminate the malignant degree of liver cancer. eHSP70/HSP70-PCs play an important role in the EMT of hepatocellular carcinoma via the p38/MAPK pathway.Hangyu LiYan LiDan LiuHongzhi SunDongming SuFuquan YangJingang LiuPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 12, p e84759 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hangyu Li Yan Li Dan Liu Hongzhi Sun Dongming Su Fuquan Yang Jingang Liu Extracellular HSP70/HSP70-PCs promote epithelial-mesenchymal transition of hepatocarcinoma cells.
description	<h4>Background</h4>Extracellular heat shock protein 70 and peptide complexes (eHSP70/HSP70-PCs) regulate a variety of biological behaviors in tumor cells. Whether eHSP70/HSP70-PCs are involved in the epithelial-mesenchymal transition (EMT) of tumor cells remains unclear.<h4>Aims</h4>To determine the effects of eHSP70/HSP70-PCs on EMT of hepatocarcinoma cells.<h4>Methods</h4>The expressions of E-cadherin, HSP70, α-smooth muscle actin protein (α-SMA) and p-p38 were detected immunohistochemically in liver cancer samples. Immunofluorescence, western blotting and real-time RT-PCR methods were used to analyze the effects of eHSP70/HSP70-PCs on the expressions of E-cadherin, α-SMA and p38/MAPK in vivo.<h4>Results</h4>HSP70, E-cadherin, α-SMA and p-p38 were elevated in hepatocellular carcinoma tissues. The expression of HSP70 was positively correlated with malignant differentiated liver carcinoma. The expressions of HSP70, α-SMA and p-p38 correlated with recurrence-free survival after resection. eHSP70/HSP70-PCs significantly promoted the expressions of α-SMA and p-p38 and reduced the expressions of E-cadherin in vivo. The effect was inhibited by SB203580.<h4>Conclusion</h4>The expressions of HSP70, E-cadherin, α-SMA and p-p38 may represent indicators of malignant potential and could discriminate the malignant degree of liver cancer. eHSP70/HSP70-PCs play an important role in the EMT of hepatocellular carcinoma via the p38/MAPK pathway.
format	article
author	Hangyu Li Yan Li Dan Liu Hongzhi Sun Dongming Su Fuquan Yang Jingang Liu
author_facet	Hangyu Li Yan Li Dan Liu Hongzhi Sun Dongming Su Fuquan Yang Jingang Liu
author_sort	Hangyu Li
title	Extracellular HSP70/HSP70-PCs promote epithelial-mesenchymal transition of hepatocarcinoma cells.
title_short	Extracellular HSP70/HSP70-PCs promote epithelial-mesenchymal transition of hepatocarcinoma cells.
title_full	Extracellular HSP70/HSP70-PCs promote epithelial-mesenchymal transition of hepatocarcinoma cells.
title_fullStr	Extracellular HSP70/HSP70-PCs promote epithelial-mesenchymal transition of hepatocarcinoma cells.
title_full_unstemmed	Extracellular HSP70/HSP70-PCs promote epithelial-mesenchymal transition of hepatocarcinoma cells.
title_sort	extracellular hsp70/hsp70-pcs promote epithelial-mesenchymal transition of hepatocarcinoma cells.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/9e55105e62d84a3f8baeafeb88da8ebb
work_keys_str_mv	AT hangyuli extracellularhsp70hsp70pcspromoteepithelialmesenchymaltransitionofhepatocarcinomacells AT yanli extracellularhsp70hsp70pcspromoteepithelialmesenchymaltransitionofhepatocarcinomacells AT danliu extracellularhsp70hsp70pcspromoteepithelialmesenchymaltransitionofhepatocarcinomacells AT hongzhisun extracellularhsp70hsp70pcspromoteepithelialmesenchymaltransitionofhepatocarcinomacells AT dongmingsu extracellularhsp70hsp70pcspromoteepithelialmesenchymaltransitionofhepatocarcinomacells AT fuquanyang extracellularhsp70hsp70pcspromoteepithelialmesenchymaltransitionofhepatocarcinomacells AT jingangliu extracellularhsp70hsp70pcspromoteepithelialmesenchymaltransitionofhepatocarcinomacells
_version_	1718421518007926784

Extracellular HSP70/HSP70-PCs promote epithelial-mesenchymal transition of hepatocarcinoma cells.

Ejemplares similares