Diagnostic accuracy of HIV test kits, Genscreen Ultra and Bioelisa
Saro Abdella Abrahim, Mulu Girma, Abebe Habteselassie, Nigussie Gezahegn, Altaye Feleke, Tezera Moshago Berheto, Minilik Demissie, Wudnesh Belete, Tekalign Deressa HIV/TB Research and Reference Laboratory Directorate, Ethiopian Public Health Institute, Addis Ababa, Ethiopia Purpose: Genetic diversit...
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Dove Medical Press
2019
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oai:doaj.org-article:9e5693fb529b4c6c9fb12967be53a1ff2021-12-02T04:37:13ZDiagnostic accuracy of HIV test kits, Genscreen Ultra and Bioelisa1179-1373https://doaj.org/article/9e5693fb529b4c6c9fb12967be53a1ff2019-02-01T00:00:00Zhttps://www.dovepress.com/diagnostic-accuracy-of-hiv-test-kits-genscreen-ultra-and-bioelisa-peer-reviewed-article-HIVhttps://doaj.org/toc/1179-1373Saro Abdella Abrahim, Mulu Girma, Abebe Habteselassie, Nigussie Gezahegn, Altaye Feleke, Tezera Moshago Berheto, Minilik Demissie, Wudnesh Belete, Tekalign Deressa HIV/TB Research and Reference Laboratory Directorate, Ethiopian Public Health Institute, Addis Ababa, Ethiopia Purpose: Genetic diversities in different countries affect the performance of HIV test kits. Therefore, WHO recommends evaluation of every HIV test kit in countries’ context before its use. Therefore, this study aimed to evaluate the performance of Genscreen ULTRA HIV Ag–Ab and Bioelisa.Materials and methods: The study had used 400 characterized plasma samples obtained from CDC Atlanta bio-bank derived from Africa, USA, and Thailand.Results: Diagnostic performance of both test kits under evaluation was assessed at 95% CI. Genscreen ULTRA HIV Ag–Ab had sensitivity and negative predictive value of 99.5% [95% CI, 97.2–99.9] and the specificity and positive predictive value of 98.5% [95% CI, 95.7–99.7]. Bioelisa HIV test kit had exhibited sensitivity and negative predictive value of 99% [95% CI, 96.4–99.7] and specificity and positive predictive value of 98.5% [95% CI, 95.7–99.7]. Both test kits were able to detect almost all samples with HIV-2, dual infections, and seroconversion.Conclusion: Both the test kits were highly sensitive and specific in detecting HIV. However, there are still few samples containing HIV antibody which were not identified by both kits. Therefore, additional screening measures should be done in using these assays for blood transfusion and organ transplantation. In addition, the study can be used as a reference by other African countries. Keywords: ELISA, sensitivity, positive predictive, specificity, validation Abrahim SAGirma MHabteselassie AGezahegn NFeleke ABerheto TMDemissie MBelete WDeressa TDove Medical PressarticleELISASensitivityPositive PredictiveBioelisaGenScreenImmunologic diseases. AllergyRC581-607ENHIV/AIDS: Research and Palliative Care, Vol Volume 11, Pp 17-22 (2019) |
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DOAJ |
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ELISA Sensitivity Positive Predictive Bioelisa GenScreen Immunologic diseases. Allergy RC581-607 |
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ELISA Sensitivity Positive Predictive Bioelisa GenScreen Immunologic diseases. Allergy RC581-607 Abrahim SA Girma M Habteselassie A Gezahegn N Feleke A Berheto TM Demissie M Belete W Deressa T Diagnostic accuracy of HIV test kits, Genscreen Ultra and Bioelisa |
| description |
Saro Abdella Abrahim, Mulu Girma, Abebe Habteselassie, Nigussie Gezahegn, Altaye Feleke, Tezera Moshago Berheto, Minilik Demissie, Wudnesh Belete, Tekalign Deressa HIV/TB Research and Reference Laboratory Directorate, Ethiopian Public Health Institute, Addis Ababa, Ethiopia Purpose: Genetic diversities in different countries affect the performance of HIV test kits. Therefore, WHO recommends evaluation of every HIV test kit in countries’ context before its use. Therefore, this study aimed to evaluate the performance of Genscreen ULTRA HIV Ag–Ab and Bioelisa.Materials and methods: The study had used 400 characterized plasma samples obtained from CDC Atlanta bio-bank derived from Africa, USA, and Thailand.Results: Diagnostic performance of both test kits under evaluation was assessed at 95% CI. Genscreen ULTRA HIV Ag–Ab had sensitivity and negative predictive value of 99.5% [95% CI, 97.2–99.9] and the specificity and positive predictive value of 98.5% [95% CI, 95.7–99.7]. Bioelisa HIV test kit had exhibited sensitivity and negative predictive value of 99% [95% CI, 96.4–99.7] and specificity and positive predictive value of 98.5% [95% CI, 95.7–99.7]. Both test kits were able to detect almost all samples with HIV-2, dual infections, and seroconversion.Conclusion: Both the test kits were highly sensitive and specific in detecting HIV. However, there are still few samples containing HIV antibody which were not identified by both kits. Therefore, additional screening measures should be done in using these assays for blood transfusion and organ transplantation. In addition, the study can be used as a reference by other African countries. Keywords: ELISA, sensitivity, positive predictive, specificity, validation
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| format |
article |
| author |
Abrahim SA Girma M Habteselassie A Gezahegn N Feleke A Berheto TM Demissie M Belete W Deressa T |
| author_facet |
Abrahim SA Girma M Habteselassie A Gezahegn N Feleke A Berheto TM Demissie M Belete W Deressa T |
| author_sort |
Abrahim SA |
| title |
Diagnostic accuracy of HIV test kits, Genscreen Ultra and Bioelisa |
| title_short |
Diagnostic accuracy of HIV test kits, Genscreen Ultra and Bioelisa |
| title_full |
Diagnostic accuracy of HIV test kits, Genscreen Ultra and Bioelisa |
| title_fullStr |
Diagnostic accuracy of HIV test kits, Genscreen Ultra and Bioelisa |
| title_full_unstemmed |
Diagnostic accuracy of HIV test kits, Genscreen Ultra and Bioelisa |
| title_sort |
diagnostic accuracy of hiv test kits, genscreen ultra and bioelisa |
| publisher |
Dove Medical Press |
| publishDate |
2019 |
| url |
https://doaj.org/article/9e5693fb529b4c6c9fb12967be53a1ff |
| work_keys_str_mv |
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