Impact of a Novel W2027L Mutation and Non-Target Site Resistance on Acetyl-CoA Carboxylase-Inhibiting Herbicides in a French Lolium multiflorum Population

Herbicides that inhibit acetyl-CoA carboxylase (ACCase) are among the few remaining options for the post-emergence control of Lolium species in small grain cereal crops. Here, we determined the mechanism of resistance to ACCase herbicides in a Lolium multiflorum...

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Autores principales:	Shiv Shankhar Kaundun, Joe Downes, Lucy Victoria Jackson, Sarah-Jane Hutchings, Eddie Mcindoe
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	herbicide acetyl-CoA carboxylase <i>Lolium multiflorum</i> mechanism of resistance I1781L I2041T Genetics QH426-470
Acceso en línea:	https://doaj.org/article/9e5f6b6126f04755aa0e6fe01502718f
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spelling	oai:doaj.org-article:9e5f6b6126f04755aa0e6fe01502718f2021-11-25T17:42:34ZImpact of a Novel W2027L Mutation and Non-Target Site Resistance on Acetyl-CoA Carboxylase-Inhibiting Herbicides in a French <i>Lolium multiflorum</i> Population10.3390/genes121118382073-4425https://doaj.org/article/9e5f6b6126f04755aa0e6fe01502718f2021-11-01T00:00:00Zhttps://www.mdpi.com/2073-4425/12/11/1838https://doaj.org/toc/2073-4425Herbicides that inhibit acetyl-CoA carboxylase (ACCase) are among the few remaining options for the post-emergence control of <i>Lolium</i> species in small grain cereal crops. Here, we determined the mechanism of resistance to ACCase herbicides in a <i>Lolium multiflorum</i> population (HGR) from France. A combined biological and molecular approach detected a novel W2027L ACCase mutation that affects aryloxyphenoxypropionate (FOP) but not cyclohexanedione (DIM) or phenylpyraxoline (DEN) subclasses of ACCase herbicides. Both the wild-type tryptophan and mutant leucine 2027-ACCase alleles could be positively detected in a single DNA-based-derived polymorphic amplified cleaved sequence (dPACS) assay that contained the targeted PCR product and a cocktail of two discriminating restriction enzymes. Additionally, we identified three well-characterised I1781L, I2041T, and D2078G ACCase target site resistance mutations as well as non-target site resistance in HGR. The non-target site component endowed high levels of resistance to FOP herbicides whilst partially impacting on the efficacy of pinoxaden and cycloxydim. This study adequately assessed the contribution of the W2027L mutation and non-target site mechanism in conferring resistance to ACCase herbicides in HGR. It also highlights the versatility and robustness of the dPACS method to simultaneously identify different resistance-causing alleles at a single ACCase codon.Shiv Shankhar KaundunJoe DownesLucy Victoria JacksonSarah-Jane HutchingsEddie McindoeMDPI AGarticleherbicideacetyl-CoA carboxylase<i>Lolium multiflorum</i>mechanism of resistanceI1781LI2041TGeneticsQH426-470ENGenes, Vol 12, Iss 1838, p 1838 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	herbicide acetyl-CoA carboxylase <i>Lolium multiflorum</i> mechanism of resistance I1781L I2041T Genetics QH426-470
spellingShingle	herbicide acetyl-CoA carboxylase <i>Lolium multiflorum</i> mechanism of resistance I1781L I2041T Genetics QH426-470 Shiv Shankhar Kaundun Joe Downes Lucy Victoria Jackson Sarah-Jane Hutchings Eddie Mcindoe Impact of a Novel W2027L Mutation and Non-Target Site Resistance on Acetyl-CoA Carboxylase-Inhibiting Herbicides in a French <i>Lolium multiflorum</i> Population
description	Herbicides that inhibit acetyl-CoA carboxylase (ACCase) are among the few remaining options for the post-emergence control of <i>Lolium</i> species in small grain cereal crops. Here, we determined the mechanism of resistance to ACCase herbicides in a <i>Lolium multiflorum</i> population (HGR) from France. A combined biological and molecular approach detected a novel W2027L ACCase mutation that affects aryloxyphenoxypropionate (FOP) but not cyclohexanedione (DIM) or phenylpyraxoline (DEN) subclasses of ACCase herbicides. Both the wild-type tryptophan and mutant leucine 2027-ACCase alleles could be positively detected in a single DNA-based-derived polymorphic amplified cleaved sequence (dPACS) assay that contained the targeted PCR product and a cocktail of two discriminating restriction enzymes. Additionally, we identified three well-characterised I1781L, I2041T, and D2078G ACCase target site resistance mutations as well as non-target site resistance in HGR. The non-target site component endowed high levels of resistance to FOP herbicides whilst partially impacting on the efficacy of pinoxaden and cycloxydim. This study adequately assessed the contribution of the W2027L mutation and non-target site mechanism in conferring resistance to ACCase herbicides in HGR. It also highlights the versatility and robustness of the dPACS method to simultaneously identify different resistance-causing alleles at a single ACCase codon.
format	article
author	Shiv Shankhar Kaundun Joe Downes Lucy Victoria Jackson Sarah-Jane Hutchings Eddie Mcindoe
author_facet	Shiv Shankhar Kaundun Joe Downes Lucy Victoria Jackson Sarah-Jane Hutchings Eddie Mcindoe
author_sort	Shiv Shankhar Kaundun
title	Impact of a Novel W2027L Mutation and Non-Target Site Resistance on Acetyl-CoA Carboxylase-Inhibiting Herbicides in a French <i>Lolium multiflorum</i> Population
title_short	Impact of a Novel W2027L Mutation and Non-Target Site Resistance on Acetyl-CoA Carboxylase-Inhibiting Herbicides in a French <i>Lolium multiflorum</i> Population
title_full	Impact of a Novel W2027L Mutation and Non-Target Site Resistance on Acetyl-CoA Carboxylase-Inhibiting Herbicides in a French <i>Lolium multiflorum</i> Population
title_fullStr	Impact of a Novel W2027L Mutation and Non-Target Site Resistance on Acetyl-CoA Carboxylase-Inhibiting Herbicides in a French <i>Lolium multiflorum</i> Population
title_full_unstemmed	Impact of a Novel W2027L Mutation and Non-Target Site Resistance on Acetyl-CoA Carboxylase-Inhibiting Herbicides in a French <i>Lolium multiflorum</i> Population
title_sort	impact of a novel w2027l mutation and non-target site resistance on acetyl-coa carboxylase-inhibiting herbicides in a french <i>lolium multiflorum</i> population
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/9e5f6b6126f04755aa0e6fe01502718f
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Impact of a Novel W2027L Mutation and Non-Target Site Resistance on Acetyl-CoA Carboxylase-Inhibiting Herbicides in a French <i>Lolium multiflorum</i> Population

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