Sterile protection against Plasmodium knowlesi in rhesus monkeys from a malaria vaccine: comparison of heterologous prime boost strategies.
Using newer vaccine platforms which have been effective against malaria in rodent models, we tested five immunization regimens against Plasmodium knowlesi in rhesus monkeys. All vaccines included the same four P. knowlesi antigens: the pre-erythrocytic antigens CSP, SSP2, and erythrocytic antigens A...
Guardado en:
| Autores principales: | , , , , , , , , , , , , , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Public Library of Science (PLoS)
2009
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/9e6e88399c3947899ccd9b821b2fadf7 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:9e6e88399c3947899ccd9b821b2fadf7 |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:9e6e88399c3947899ccd9b821b2fadf72021-11-25T06:21:07ZSterile protection against Plasmodium knowlesi in rhesus monkeys from a malaria vaccine: comparison of heterologous prime boost strategies.1932-620310.1371/journal.pone.0006559https://doaj.org/article/9e6e88399c3947899ccd9b821b2fadf72009-08-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19668343/?tool=EBIhttps://doaj.org/toc/1932-6203Using newer vaccine platforms which have been effective against malaria in rodent models, we tested five immunization regimens against Plasmodium knowlesi in rhesus monkeys. All vaccines included the same four P. knowlesi antigens: the pre-erythrocytic antigens CSP, SSP2, and erythrocytic antigens AMA1, MSP1. We used four vaccine platforms for prime or boost vaccinations: plasmids (DNA), alphavirus replicons (VRP), attenuated adenovirus serotype 5 (Ad), or attenuated poxvirus (Pox). These four platforms combined to produce five different prime/boost vaccine regimens: Pox alone, VRP/Pox, VRP/Ad, Ad/Pox, and DNA/Pox. Five rhesus monkeys were immunized with each regimen, and five Control monkeys received a mock vaccination. The time to complete vaccinations was 420 days. All monkeys were challenged twice with 100 P. knowlesi sporozoites given IV. The first challenge was given 12 days after the last vaccination, and the monkeys receiving the DNA/Pox vaccine were the best protected, with 3/5 monkeys sterilely protected and 1/5 monkeys that self-cured its parasitemia. There was no protection in monkeys that received Pox malaria vaccine alone without previous priming. The second sporozoite challenge was given 4 months after the first. All 4 monkeys that were protected in the first challenge developed malaria in the second challenge. DNA, VRP and Ad5 vaccines all primed monkeys for strong immune responses after the Pox boost. We discuss the high level but short duration of protection in this experiment and the possible benefits of the long interval between prime and boost.George JiangMeng ShiSolomon ContehNancy RichieGlenna BananiaHarini GeneshanAnais ValenciaPriti SinghJoao AguiarKeith LimbachKurt I KamrudJonathan RaynerJonathan SmithJoseph T BruderC Richter KingTakafumi TsuboiSatoru TakeoYaeta EndoDenise L DoolanThomas L RichieWalter R WeissPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 4, Iss 8, p e6559 (2009) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Medicine R Science Q |
| spellingShingle |
Medicine R Science Q George Jiang Meng Shi Solomon Conteh Nancy Richie Glenna Banania Harini Geneshan Anais Valencia Priti Singh Joao Aguiar Keith Limbach Kurt I Kamrud Jonathan Rayner Jonathan Smith Joseph T Bruder C Richter King Takafumi Tsuboi Satoru Takeo Yaeta Endo Denise L Doolan Thomas L Richie Walter R Weiss Sterile protection against Plasmodium knowlesi in rhesus monkeys from a malaria vaccine: comparison of heterologous prime boost strategies. |
| description |
Using newer vaccine platforms which have been effective against malaria in rodent models, we tested five immunization regimens against Plasmodium knowlesi in rhesus monkeys. All vaccines included the same four P. knowlesi antigens: the pre-erythrocytic antigens CSP, SSP2, and erythrocytic antigens AMA1, MSP1. We used four vaccine platforms for prime or boost vaccinations: plasmids (DNA), alphavirus replicons (VRP), attenuated adenovirus serotype 5 (Ad), or attenuated poxvirus (Pox). These four platforms combined to produce five different prime/boost vaccine regimens: Pox alone, VRP/Pox, VRP/Ad, Ad/Pox, and DNA/Pox. Five rhesus monkeys were immunized with each regimen, and five Control monkeys received a mock vaccination. The time to complete vaccinations was 420 days. All monkeys were challenged twice with 100 P. knowlesi sporozoites given IV. The first challenge was given 12 days after the last vaccination, and the monkeys receiving the DNA/Pox vaccine were the best protected, with 3/5 monkeys sterilely protected and 1/5 monkeys that self-cured its parasitemia. There was no protection in monkeys that received Pox malaria vaccine alone without previous priming. The second sporozoite challenge was given 4 months after the first. All 4 monkeys that were protected in the first challenge developed malaria in the second challenge. DNA, VRP and Ad5 vaccines all primed monkeys for strong immune responses after the Pox boost. We discuss the high level but short duration of protection in this experiment and the possible benefits of the long interval between prime and boost. |
| format |
article |
| author |
George Jiang Meng Shi Solomon Conteh Nancy Richie Glenna Banania Harini Geneshan Anais Valencia Priti Singh Joao Aguiar Keith Limbach Kurt I Kamrud Jonathan Rayner Jonathan Smith Joseph T Bruder C Richter King Takafumi Tsuboi Satoru Takeo Yaeta Endo Denise L Doolan Thomas L Richie Walter R Weiss |
| author_facet |
George Jiang Meng Shi Solomon Conteh Nancy Richie Glenna Banania Harini Geneshan Anais Valencia Priti Singh Joao Aguiar Keith Limbach Kurt I Kamrud Jonathan Rayner Jonathan Smith Joseph T Bruder C Richter King Takafumi Tsuboi Satoru Takeo Yaeta Endo Denise L Doolan Thomas L Richie Walter R Weiss |
| author_sort |
George Jiang |
| title |
Sterile protection against Plasmodium knowlesi in rhesus monkeys from a malaria vaccine: comparison of heterologous prime boost strategies. |
| title_short |
Sterile protection against Plasmodium knowlesi in rhesus monkeys from a malaria vaccine: comparison of heterologous prime boost strategies. |
| title_full |
Sterile protection against Plasmodium knowlesi in rhesus monkeys from a malaria vaccine: comparison of heterologous prime boost strategies. |
| title_fullStr |
Sterile protection against Plasmodium knowlesi in rhesus monkeys from a malaria vaccine: comparison of heterologous prime boost strategies. |
| title_full_unstemmed |
Sterile protection against Plasmodium knowlesi in rhesus monkeys from a malaria vaccine: comparison of heterologous prime boost strategies. |
| title_sort |
sterile protection against plasmodium knowlesi in rhesus monkeys from a malaria vaccine: comparison of heterologous prime boost strategies. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2009 |
| url |
https://doaj.org/article/9e6e88399c3947899ccd9b821b2fadf7 |
| work_keys_str_mv |
AT georgejiang sterileprotectionagainstplasmodiumknowlesiinrhesusmonkeysfromamalariavaccinecomparisonofheterologousprimebooststrategies AT mengshi sterileprotectionagainstplasmodiumknowlesiinrhesusmonkeysfromamalariavaccinecomparisonofheterologousprimebooststrategies AT solomonconteh sterileprotectionagainstplasmodiumknowlesiinrhesusmonkeysfromamalariavaccinecomparisonofheterologousprimebooststrategies AT nancyrichie sterileprotectionagainstplasmodiumknowlesiinrhesusmonkeysfromamalariavaccinecomparisonofheterologousprimebooststrategies AT glennabanania sterileprotectionagainstplasmodiumknowlesiinrhesusmonkeysfromamalariavaccinecomparisonofheterologousprimebooststrategies AT harinigeneshan sterileprotectionagainstplasmodiumknowlesiinrhesusmonkeysfromamalariavaccinecomparisonofheterologousprimebooststrategies AT anaisvalencia sterileprotectionagainstplasmodiumknowlesiinrhesusmonkeysfromamalariavaccinecomparisonofheterologousprimebooststrategies AT pritisingh sterileprotectionagainstplasmodiumknowlesiinrhesusmonkeysfromamalariavaccinecomparisonofheterologousprimebooststrategies AT joaoaguiar sterileprotectionagainstplasmodiumknowlesiinrhesusmonkeysfromamalariavaccinecomparisonofheterologousprimebooststrategies AT keithlimbach sterileprotectionagainstplasmodiumknowlesiinrhesusmonkeysfromamalariavaccinecomparisonofheterologousprimebooststrategies AT kurtikamrud sterileprotectionagainstplasmodiumknowlesiinrhesusmonkeysfromamalariavaccinecomparisonofheterologousprimebooststrategies AT jonathanrayner sterileprotectionagainstplasmodiumknowlesiinrhesusmonkeysfromamalariavaccinecomparisonofheterologousprimebooststrategies AT jonathansmith sterileprotectionagainstplasmodiumknowlesiinrhesusmonkeysfromamalariavaccinecomparisonofheterologousprimebooststrategies AT josephtbruder sterileprotectionagainstplasmodiumknowlesiinrhesusmonkeysfromamalariavaccinecomparisonofheterologousprimebooststrategies AT crichterking sterileprotectionagainstplasmodiumknowlesiinrhesusmonkeysfromamalariavaccinecomparisonofheterologousprimebooststrategies AT takafumitsuboi sterileprotectionagainstplasmodiumknowlesiinrhesusmonkeysfromamalariavaccinecomparisonofheterologousprimebooststrategies AT satorutakeo sterileprotectionagainstplasmodiumknowlesiinrhesusmonkeysfromamalariavaccinecomparisonofheterologousprimebooststrategies AT yaetaendo sterileprotectionagainstplasmodiumknowlesiinrhesusmonkeysfromamalariavaccinecomparisonofheterologousprimebooststrategies AT deniseldoolan sterileprotectionagainstplasmodiumknowlesiinrhesusmonkeysfromamalariavaccinecomparisonofheterologousprimebooststrategies AT thomaslrichie sterileprotectionagainstplasmodiumknowlesiinrhesusmonkeysfromamalariavaccinecomparisonofheterologousprimebooststrategies AT walterrweiss sterileprotectionagainstplasmodiumknowlesiinrhesusmonkeysfromamalariavaccinecomparisonofheterologousprimebooststrategies |
| _version_ |
1718413816965890048 |