Influence of sugars and osmoregulators on motility and viability of cooled and frozen-thawed ram semen

<b>The aim of the study was to determine the effect of sugars and osmoregulators on the viability of frozen-thawed ram semen in a Tris-based diluent. Two experiments were conducted to evaluate the effect of sugars (fructose, glucose, sucrose and raffinose) and two osmoregulators (hypotaurine a...

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Autores principales:	Khaled El-Shahat, Magdi Waheed, Amal Hasan Ali, Abdel -Aziz Sallam, Badr El-Saidy
Formato:	article
Lenguaje:	EN PL
Publicado:	Polish Society of Animal Production 2020
Materias:	Ram semen Sugars Osmoregulator Dilution Freezing Zoology QL1-991
Acceso en línea:	https://doaj.org/article/9e6f531710c94fa0a95e45a5d8863258
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spelling	oai:doaj.org-article:9e6f531710c94fa0a95e45a5d88632582021-11-10T16:24:42ZInfluence of sugars and osmoregulators on motility and viability of cooled and frozen-thawed ram semen1733-73052719-393410.5604/01.3001.0014.2020https://doaj.org/article/9e6f531710c94fa0a95e45a5d88632582020-06-01T00:00:00Zhttp://zootechnical.com/gicid/01.3001.0014.2020http://zootechnical.com/gicid/pdf/01.3001.0014.2020https://doaj.org/toc/1733-7305https://doaj.org/toc/2719-3934<b>The aim of the study was to determine the effect of sugars and osmoregulators on the viability of frozen-thawed ram semen in a Tris-based diluent. Two experiments were conducted to evaluate the effect of sugars (fructose, glucose, sucrose and raffinose) and two osmoregulators (hypotaurine and taurine) on the viability of ram sperm. In experiment 1, each of the four sugars (fructose, glucose, sucrose and raffinose) was added (500 mg%) to the diluents. Semen was collected from 15 rams once a week using an artificial vagina, diluted, cooled slowly to 5°C over 2 h, frozen in the form of pellets, and plunged into liquid nitrogen. The frozen semen was thawed, and sperm viability was calculated 3 h after thawing. In experiment 2, two osmoregulators, hypotaurine (0.20 and 0.40 mg/ml) and taurine (2 and 4 mg/ml), were added to Tris-based raffinose diluent. Motility and viability rates were calculated. The results showed that motility was gradually significantly (P < 0.05) improved by using 500 mg% raffinose in the Tris-based diluents after dilution at 30°C and before freezing at 5°C. Post-thawing motility and viability rates were highest (P<0.05) when raffinose was used in Tris-based diluent for cryopreservation of ram semen. In vitro supplementation of the semen diluent with 4 mg/ml taurine had a beneficial effect on the motility and viability of ram spermatozoa after dilution at 30°C and before freezing at 5°C. The same trend was observed after freezing-thawing and 3 h post-thawing. In conclusion, 500 mg% raffinose was a more suitable sugar component for treatment of ram spermatozoa in Tris diluent than fructose or glucose, while 4 mg/ml of taurine in Tris-raffinose medium exerted a beneficial effect on the motility and viability of ram sperm at cooling and post-thawing.</b> Khaled El-ShahatMagdi WaheedAmal Hasan AliAbdel -Aziz SallamBadr El-SaidyPolish Society of Animal ProductionarticleRam semenSugarsOsmoregulatorDilutionFreezing ZoologyQL1-991ENPLRoczniki Naukowe Polskiego Towarzystwa Zootechnicznego, Vol 16, Iss 2, Pp 51-57 (2020)
institution	DOAJ
collection	DOAJ
language	EN PL
topic	Ram semen Sugars Osmoregulator Dilution Freezing Zoology QL1-991
spellingShingle	Ram semen Sugars Osmoregulator Dilution Freezing Zoology QL1-991 Khaled El-Shahat Magdi Waheed Amal Hasan Ali Abdel -Aziz Sallam Badr El-Saidy Influence of sugars and osmoregulators on motility and viability of cooled and frozen-thawed ram semen
description	<b>The aim of the study was to determine the effect of sugars and osmoregulators on the viability of frozen-thawed ram semen in a Tris-based diluent. Two experiments were conducted to evaluate the effect of sugars (fructose, glucose, sucrose and raffinose) and two osmoregulators (hypotaurine and taurine) on the viability of ram sperm. In experiment 1, each of the four sugars (fructose, glucose, sucrose and raffinose) was added (500 mg%) to the diluents. Semen was collected from 15 rams once a week using an artificial vagina, diluted, cooled slowly to 5°C over 2 h, frozen in the form of pellets, and plunged into liquid nitrogen. The frozen semen was thawed, and sperm viability was calculated 3 h after thawing. In experiment 2, two osmoregulators, hypotaurine (0.20 and 0.40 mg/ml) and taurine (2 and 4 mg/ml), were added to Tris-based raffinose diluent. Motility and viability rates were calculated. The results showed that motility was gradually significantly (P < 0.05) improved by using 500 mg% raffinose in the Tris-based diluents after dilution at 30°C and before freezing at 5°C. Post-thawing motility and viability rates were highest (P<0.05) when raffinose was used in Tris-based diluent for cryopreservation of ram semen. In vitro supplementation of the semen diluent with 4 mg/ml taurine had a beneficial effect on the motility and viability of ram spermatozoa after dilution at 30°C and before freezing at 5°C. The same trend was observed after freezing-thawing and 3 h post-thawing. In conclusion, 500 mg% raffinose was a more suitable sugar component for treatment of ram spermatozoa in Tris diluent than fructose or glucose, while 4 mg/ml of taurine in Tris-raffinose medium exerted a beneficial effect on the motility and viability of ram sperm at cooling and post-thawing.</b>
format	article
author	Khaled El-Shahat Magdi Waheed Amal Hasan Ali Abdel -Aziz Sallam Badr El-Saidy
author_facet	Khaled El-Shahat Magdi Waheed Amal Hasan Ali Abdel -Aziz Sallam Badr El-Saidy
author_sort	Khaled El-Shahat
title	Influence of sugars and osmoregulators on motility and viability of cooled and frozen-thawed ram semen
title_short	Influence of sugars and osmoregulators on motility and viability of cooled and frozen-thawed ram semen
title_full	Influence of sugars and osmoregulators on motility and viability of cooled and frozen-thawed ram semen
title_fullStr	Influence of sugars and osmoregulators on motility and viability of cooled and frozen-thawed ram semen
title_full_unstemmed	Influence of sugars and osmoregulators on motility and viability of cooled and frozen-thawed ram semen
title_sort	influence of sugars and osmoregulators on motility and viability of cooled and frozen-thawed ram semen
publisher	Polish Society of Animal Production
publishDate	2020
url	https://doaj.org/article/9e6f531710c94fa0a95e45a5d8863258
work_keys_str_mv	AT khaledelshahat influenceofsugarsandosmoregulatorsonmotilityandviabilityofcooledandfrozenthawedramsemen AT magdiwaheed influenceofsugarsandosmoregulatorsonmotilityandviabilityofcooledandfrozenthawedramsemen AT amalhasanali influenceofsugarsandosmoregulatorsonmotilityandviabilityofcooledandfrozenthawedramsemen AT abdelazizsallam influenceofsugarsandosmoregulatorsonmotilityandviabilityofcooledandfrozenthawedramsemen AT badrelsaidy influenceofsugarsandosmoregulatorsonmotilityandviabilityofcooledandfrozenthawedramsemen
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Influence of sugars and osmoregulators on motility and viability of cooled and frozen-thawed ram semen

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