Assessing ecological status in karstic lakes through the integration of phytoplankton functional groups, morphological approach and environmental DNA metabarcoding

Phytoplankton is one of the key Biological Quality Elements within the Water Framework Directive, used to assess the ecological status of surface water bodies. Water samples for phytoplankton identification were collected from April to September at a total of eight sampling sites in all six Croatian...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Nikola Hanžek, Marija Gligora Udovič, Katarina Kajan, Gábor Borics, Gábor Várbíró, Thorsten Stoeck, Petar Žutinić, Sandi Orlić, Igor Stanković
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Croatian natural karstic lakes 18S rRNA V9 Molecular OTU Hungarian lake phytoplankton index Chlorophyll a Water framework directive Ecology QH540-549.5
Acceso en línea:	https://doaj.org/article/9e7606429c6a4b118983a4674d5941a0
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:9e7606429c6a4b118983a4674d5941a0
record_format	dspace
spelling	oai:doaj.org-article:9e7606429c6a4b118983a4674d5941a02021-12-01T04:59:54ZAssessing ecological status in karstic lakes through the integration of phytoplankton functional groups, morphological approach and environmental DNA metabarcoding1470-160X10.1016/j.ecolind.2021.108166https://doaj.org/article/9e7606429c6a4b118983a4674d5941a02021-11-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S1470160X21008311https://doaj.org/toc/1470-160XPhytoplankton is one of the key Biological Quality Elements within the Water Framework Directive, used to assess the ecological status of surface water bodies. Water samples for phytoplankton identification were collected from April to September at a total of eight sampling sites in all six Croatian natural karstic lakes with an area greater than 0.5 km2. The main objective was to show the comparability of environmental DNA metabarcoding (Illumina sequencing using the hypervariable V9 region of the eukaryotic SSU rRNA gene) with morphologically based assessment and its applicability in assessing the ecological status of lakes. The value of Hungarian lake phytoplankton index (HLPI) indicating the final ecological status was calculated for both datasets using biomass and composition metrics. Chlorophyll a concentration measured using Ultra-High-Performance Liquid Chromatography and Spectrophotometer giving two biomass metrics along with the functional group approach (FG) as the composition metric for the complete taxa/operational taxonomic units (OTUs) lists as well as for the taxa/OTUs that contributed more than 5% to the total biomass/number of amplicons gave four to four HLPI values per sample. HLPI values from both approaches were highly correlated (Pearson's r > 0.92) and classified into good or high ecological status, although different compositions and proportions of FGs were recorded, thus giving the important role to the equal or similar factors assignment to different FGs with similar ecological demands and favourable habitats. In 89% of the samples, HLPI values indicate an equal range of ecological status and most differences were found due to different methods of Chlorophyll a measurement. Different composition metrics between approaches showed significant differences (p < 0.05) only in lakes Prošće and Crniševo. This study showed the applicability of the V9 region of 18S rRNA in ecological status assessment for oligotrophic and mesotrophic lakes due to the comparable results between approaches, but further development and standardization of eDNA metabarcoding are needed for the implementation in routine monitoring programs.Nikola HanžekMarija Gligora UdovičKatarina KajanGábor BoricsGábor VárbíróThorsten StoeckPetar ŽutinićSandi OrlićIgor StankovićElsevierarticleCroatian natural karstic lakes18S rRNA V9Molecular OTUHungarian lake phytoplankton indexChlorophyll aWater framework directiveEcologyQH540-549.5ENEcological Indicators, Vol 131, Iss , Pp 108166- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Croatian natural karstic lakes 18S rRNA V9 Molecular OTU Hungarian lake phytoplankton index Chlorophyll a Water framework directive Ecology QH540-549.5
spellingShingle	Croatian natural karstic lakes 18S rRNA V9 Molecular OTU Hungarian lake phytoplankton index Chlorophyll a Water framework directive Ecology QH540-549.5 Nikola Hanžek Marija Gligora Udovič Katarina Kajan Gábor Borics Gábor Várbíró Thorsten Stoeck Petar Žutinić Sandi Orlić Igor Stanković Assessing ecological status in karstic lakes through the integration of phytoplankton functional groups, morphological approach and environmental DNA metabarcoding
description	Phytoplankton is one of the key Biological Quality Elements within the Water Framework Directive, used to assess the ecological status of surface water bodies. Water samples for phytoplankton identification were collected from April to September at a total of eight sampling sites in all six Croatian natural karstic lakes with an area greater than 0.5 km2. The main objective was to show the comparability of environmental DNA metabarcoding (Illumina sequencing using the hypervariable V9 region of the eukaryotic SSU rRNA gene) with morphologically based assessment and its applicability in assessing the ecological status of lakes. The value of Hungarian lake phytoplankton index (HLPI) indicating the final ecological status was calculated for both datasets using biomass and composition metrics. Chlorophyll a concentration measured using Ultra-High-Performance Liquid Chromatography and Spectrophotometer giving two biomass metrics along with the functional group approach (FG) as the composition metric for the complete taxa/operational taxonomic units (OTUs) lists as well as for the taxa/OTUs that contributed more than 5% to the total biomass/number of amplicons gave four to four HLPI values per sample. HLPI values from both approaches were highly correlated (Pearson's r > 0.92) and classified into good or high ecological status, although different compositions and proportions of FGs were recorded, thus giving the important role to the equal or similar factors assignment to different FGs with similar ecological demands and favourable habitats. In 89% of the samples, HLPI values indicate an equal range of ecological status and most differences were found due to different methods of Chlorophyll a measurement. Different composition metrics between approaches showed significant differences (p < 0.05) only in lakes Prošće and Crniševo. This study showed the applicability of the V9 region of 18S rRNA in ecological status assessment for oligotrophic and mesotrophic lakes due to the comparable results between approaches, but further development and standardization of eDNA metabarcoding are needed for the implementation in routine monitoring programs.
format	article
author	Nikola Hanžek Marija Gligora Udovič Katarina Kajan Gábor Borics Gábor Várbíró Thorsten Stoeck Petar Žutinić Sandi Orlić Igor Stanković
author_facet	Nikola Hanžek Marija Gligora Udovič Katarina Kajan Gábor Borics Gábor Várbíró Thorsten Stoeck Petar Žutinić Sandi Orlić Igor Stanković
author_sort	Nikola Hanžek
title	Assessing ecological status in karstic lakes through the integration of phytoplankton functional groups, morphological approach and environmental DNA metabarcoding
title_short	Assessing ecological status in karstic lakes through the integration of phytoplankton functional groups, morphological approach and environmental DNA metabarcoding
title_full	Assessing ecological status in karstic lakes through the integration of phytoplankton functional groups, morphological approach and environmental DNA metabarcoding
title_fullStr	Assessing ecological status in karstic lakes through the integration of phytoplankton functional groups, morphological approach and environmental DNA metabarcoding
title_full_unstemmed	Assessing ecological status in karstic lakes through the integration of phytoplankton functional groups, morphological approach and environmental DNA metabarcoding
title_sort	assessing ecological status in karstic lakes through the integration of phytoplankton functional groups, morphological approach and environmental dna metabarcoding
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/9e7606429c6a4b118983a4674d5941a0
work_keys_str_mv	AT nikolahanzek assessingecologicalstatusinkarsticlakesthroughtheintegrationofphytoplanktonfunctionalgroupsmorphologicalapproachandenvironmentaldnametabarcoding AT marijagligoraudovic assessingecologicalstatusinkarsticlakesthroughtheintegrationofphytoplanktonfunctionalgroupsmorphologicalapproachandenvironmentaldnametabarcoding AT katarinakajan assessingecologicalstatusinkarsticlakesthroughtheintegrationofphytoplanktonfunctionalgroupsmorphologicalapproachandenvironmentaldnametabarcoding AT gaborborics assessingecologicalstatusinkarsticlakesthroughtheintegrationofphytoplanktonfunctionalgroupsmorphologicalapproachandenvironmentaldnametabarcoding AT gaborvarbiro assessingecologicalstatusinkarsticlakesthroughtheintegrationofphytoplanktonfunctionalgroupsmorphologicalapproachandenvironmentaldnametabarcoding AT thorstenstoeck assessingecologicalstatusinkarsticlakesthroughtheintegrationofphytoplanktonfunctionalgroupsmorphologicalapproachandenvironmentaldnametabarcoding AT petarzutinic assessingecologicalstatusinkarsticlakesthroughtheintegrationofphytoplanktonfunctionalgroupsmorphologicalapproachandenvironmentaldnametabarcoding AT sandiorlic assessingecologicalstatusinkarsticlakesthroughtheintegrationofphytoplanktonfunctionalgroupsmorphologicalapproachandenvironmentaldnametabarcoding AT igorstankovic assessingecologicalstatusinkarsticlakesthroughtheintegrationofphytoplanktonfunctionalgroupsmorphologicalapproachandenvironmentaldnametabarcoding
_version_	1718405617337499648

Assessing ecological status in karstic lakes through the integration of phytoplankton functional groups, morphological approach and environmental DNA metabarcoding

Ejemplares similares