Electromagnetic energy (670 nm) stimulates vasodilation through activation of the large conductance potassium channel (BKCa).

<h4>Introduction</h4>Peripheral artery disease (PAD) is a highly morbid condition in which impaired blood flow to the limbs leads to pain and tissue loss. Previously we identified 670 nm electromagnetic energy (R/NIR) to increase nitric oxide levels in cells and tissue. NO elicits relaxa...

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Autores principales:	Debebe Gebremendhin, Brian Lindemer, Dorothee Weihrauch, David R Harder, Nicole L Lohr
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9e7728b8a5b842d696c2644ca42a9bec
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spelling	oai:doaj.org-article:9e7728b8a5b842d696c2644ca42a9bec2021-12-02T20:07:57ZElectromagnetic energy (670 nm) stimulates vasodilation through activation of the large conductance potassium channel (BKCa).1932-620310.1371/journal.pone.0257896https://doaj.org/article/9e7728b8a5b842d696c2644ca42a9bec2021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0257896https://doaj.org/toc/1932-6203<h4>Introduction</h4>Peripheral artery disease (PAD) is a highly morbid condition in which impaired blood flow to the limbs leads to pain and tissue loss. Previously we identified 670 nm electromagnetic energy (R/NIR) to increase nitric oxide levels in cells and tissue. NO elicits relaxation of smooth muscle (SMC) by stimulating potassium efflux and membrane hyperpolarization. The actions of energy on ion channel activity have yet to be explored. Here we hypothesized R/NIR stimulates vasodilation through activation of potassium channels in SMC.<h4>Methods</h4>Femoral arteries or facial arteries from C57Bl/6 and Slo1-/- mice were isolated, pressurized to 60 mmHg, pre-constricted with U46619, and irradiated twice with energy R/NIR (10 mW/cm2 for 5 min) with a 10 min dark period between irradiations. Single-channel K+ currents were recorded at room temperature from cell-attached and excised inside-out membrane patches of freshly isolated mouse femoral arterial muscle cells using the patch-clamp technique.<h4>Results</h4>R/NIR stimulated vasodilation requires functional activation of the large conductance potassium channels. There is a voltage dependent outward current in SMC with light stimulation, which is due to increases in the open state probability of channel opening. R/NIR modulation of channel opening is eliminated pharmacologically (paxilline) and genetically (BKca α subunit knockout). There is no direct action of light to modulate channel activity as excised patches did not increase the open state probability of channel opening.<h4>Conclusion</h4>R/NIR vasodilation requires indirect activation of the BKca channel.Debebe GebremendhinBrian LindemerDorothee WeihrauchDavid R HarderNicole L LohrPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 10, p e0257896 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Debebe Gebremendhin Brian Lindemer Dorothee Weihrauch David R Harder Nicole L Lohr Electromagnetic energy (670 nm) stimulates vasodilation through activation of the large conductance potassium channel (BKCa).
description	<h4>Introduction</h4>Peripheral artery disease (PAD) is a highly morbid condition in which impaired blood flow to the limbs leads to pain and tissue loss. Previously we identified 670 nm electromagnetic energy (R/NIR) to increase nitric oxide levels in cells and tissue. NO elicits relaxation of smooth muscle (SMC) by stimulating potassium efflux and membrane hyperpolarization. The actions of energy on ion channel activity have yet to be explored. Here we hypothesized R/NIR stimulates vasodilation through activation of potassium channels in SMC.<h4>Methods</h4>Femoral arteries or facial arteries from C57Bl/6 and Slo1-/- mice were isolated, pressurized to 60 mmHg, pre-constricted with U46619, and irradiated twice with energy R/NIR (10 mW/cm2 for 5 min) with a 10 min dark period between irradiations. Single-channel K+ currents were recorded at room temperature from cell-attached and excised inside-out membrane patches of freshly isolated mouse femoral arterial muscle cells using the patch-clamp technique.<h4>Results</h4>R/NIR stimulated vasodilation requires functional activation of the large conductance potassium channels. There is a voltage dependent outward current in SMC with light stimulation, which is due to increases in the open state probability of channel opening. R/NIR modulation of channel opening is eliminated pharmacologically (paxilline) and genetically (BKca α subunit knockout). There is no direct action of light to modulate channel activity as excised patches did not increase the open state probability of channel opening.<h4>Conclusion</h4>R/NIR vasodilation requires indirect activation of the BKca channel.
format	article
author	Debebe Gebremendhin Brian Lindemer Dorothee Weihrauch David R Harder Nicole L Lohr
author_facet	Debebe Gebremendhin Brian Lindemer Dorothee Weihrauch David R Harder Nicole L Lohr
author_sort	Debebe Gebremendhin
title	Electromagnetic energy (670 nm) stimulates vasodilation through activation of the large conductance potassium channel (BKCa).
title_short	Electromagnetic energy (670 nm) stimulates vasodilation through activation of the large conductance potassium channel (BKCa).
title_full	Electromagnetic energy (670 nm) stimulates vasodilation through activation of the large conductance potassium channel (BKCa).
title_fullStr	Electromagnetic energy (670 nm) stimulates vasodilation through activation of the large conductance potassium channel (BKCa).
title_full_unstemmed	Electromagnetic energy (670 nm) stimulates vasodilation through activation of the large conductance potassium channel (BKCa).
title_sort	electromagnetic energy (670 nm) stimulates vasodilation through activation of the large conductance potassium channel (bkca).
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/9e7728b8a5b842d696c2644ca42a9bec
work_keys_str_mv	AT debebegebremendhin electromagneticenergy670nmstimulatesvasodilationthroughactivationofthelargeconductancepotassiumchannelbkca AT brianlindemer electromagneticenergy670nmstimulatesvasodilationthroughactivationofthelargeconductancepotassiumchannelbkca AT dorotheeweihrauch electromagneticenergy670nmstimulatesvasodilationthroughactivationofthelargeconductancepotassiumchannelbkca AT davidrharder electromagneticenergy670nmstimulatesvasodilationthroughactivationofthelargeconductancepotassiumchannelbkca AT nicolellohr electromagneticenergy670nmstimulatesvasodilationthroughactivationofthelargeconductancepotassiumchannelbkca
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Electromagnetic energy (670 nm) stimulates vasodilation through activation of the large conductance potassium channel (BKCa).

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