Electrospun silk fibroin/poly(lactide-co-ε-caprolactone) nanofibrous scaffolds for bone regeneration

Electrospun silk fibroin/poly(lactide-co-ε-caprolactone) nanofibrous scaffolds for bone regeneration

Zi Wang,1,* Ming Lin,1,* Qing Xie,1 Hao Sun,1 Yazhuo Huang,1 DanDan Zhang,1 Zhang Yu,1 Xiaoping Bi,1 Junzhao Chen,1 Jing Wang,2 Wodong Shi,1 Ping Gu,1 Xianqun Fan1 1Department of Ophthalmology, Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, 2Biomaterials and Ti...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Wang Z, Lin M, Xie Q, Sun H, Huang Y, Zhang DD, Yu Z, Bi X, Chen J, Wang J, Shi W, Gu P, Fan X
Formato: article
Lenguaje:EN
Publicado: Dove Medical Press 2016
Materias:
human adipose-derived mesenchymal stem cells (hADSCs)
silk fibrin (SF)
poly(lactide-co-e-caprolactone) (PLCL)
proliferation
osteogenesis
Medicine (General)
R5-920
Acceso en línea:https://doaj.org/article/9e7d56de88974ceeac98e3a4f6684ec3
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
id oai:doaj.org-article:9e7d56de88974ceeac98e3a4f6684ec3
record_format dspace
spelling oai:doaj.org-article:9e7d56de88974ceeac98e3a4f6684ec32021-12-02T02:10:34ZElectrospun silk fibroin/poly(lactide-co-ε-caprolactone) nanofibrous scaffolds for bone regeneration1178-2013https://doaj.org/article/9e7d56de88974ceeac98e3a4f6684ec32016-04-01T00:00:00Zhttps://www.dovepress.com/electrospun-silk-fibroinpolylactide-co-epsilon-caprolactone-nanofibrou-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Zi Wang,1,* Ming Lin,1,* Qing Xie,1 Hao Sun,1 Yazhuo Huang,1 DanDan Zhang,1 Zhang Yu,1 Xiaoping Bi,1 Junzhao Chen,1 Jing Wang,2 Wodong Shi,1 Ping Gu,1 Xianqun Fan1 1Department of Ophthalmology, Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, 2Biomaterials and Tissue Engineering Laboratory, College of Chemistry & Chemical Engineering and Biotechnology, Donghua University, Shanghai, People’s Republic of China *These authors contributed equally to this work Background: Tissue engineering has become a promising therapeutic approach for bone regeneration. Nanofibrous scaffolds have attracted great interest mainly due to their structural similarity to natural extracellular matrix (ECM). Poly(lactide-co-ε-caprolactone) (PLCL) has been successfully used in bone regeneration, but PLCL polymers are inert and lack natural cell recognition sites, and the surface of PLCL scaffold is hydrophobic. Silk fibroin (SF) is a kind of natural polymer with inherent bioactivity, and supports mesenchymal stem cell attachment, osteogenesis, and ECM deposition. Therefore, we fabricated hybrid nanofibrous scaffolds by adding different weight ratios of SF to PLCL in order to find a scaffold with improved properties for bone regeneration.Methods: Hybrid nanofibrous scaffolds were fabricated by blending different weight ratios of SF with PLCL. Human adipose-derived stem cells (hADSCs) were seeded on SF/PLCL nanofibrous scaffolds of various ratios for a systematic evaluation of cell adhesion, proliferation, cytotoxicity, and osteogenic differentiation; the efficacy of the composite of hADSCs and scaffolds in repairing critical-sized calvarial defects in rats was investigated.Results: The SF/PLCL (50/50) scaffold exhibited favorable tensile strength, surface roughness, and hydrophilicity, which facilitated cell adhesion and proliferation. Moreover, the SF/PLCL (50/50) scaffold promoted the osteogenic differentiation of hADSCs by elevating the expression levels of osteogenic marker genes such as BSP, Ocn, Col1A1, and OPN and enhanced ECM mineralization. In vivo assays showed that SF/PLCL (50/50) scaffold improved the repair of the critical-sized calvarial defect in rats, resulting in increased bone volume, higher trabecular number, enhanced bone mineral density, and increased new bone areas, compared with the pure PLCL scaffold.Conclusion: The SF/PLCL (50/50) nanofibrous scaffold facilitated hADSC proliferation and osteogenic differentiation in vitro and further promoted new bone formation in vivo, suggesting that the SF/PLCL (50/50) nanofibrous scaffold holds great potential in bone tissue regeneration. Keywords: human adipose-derived mesenchymal stem cells, silk fibrin, poly(lactide-co-ε-caprolactone), proliferation, osteogenesisWang ZLin MXie QSun HHuang YZhang DDYu ZBi XChen JWang JShi WGu PFan XDove Medical Pressarticlehuman adipose-derived mesenchymal stem cells (hADSCs)silk fibrin (SF)poly(lactide-co-e-caprolactone) (PLCL)proliferationosteogenesisMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2016, Iss default, Pp 1483-1500 (2016)
institution DOAJ
collection DOAJ
language EN
topic human adipose-derived mesenchymal stem cells (hADSCs)
silk fibrin (SF)
poly(lactide-co-e-caprolactone) (PLCL)
proliferation
osteogenesis
Medicine (General)
R5-920
spellingShingle human adipose-derived mesenchymal stem cells (hADSCs)
silk fibrin (SF)
poly(lactide-co-e-caprolactone) (PLCL)
proliferation
osteogenesis
Medicine (General)
R5-920
Wang Z
Lin M
Xie Q
Sun H
Huang Y
Zhang DD
Yu Z
Bi X
Chen J
Wang J
Shi W
Gu P
Fan X
Electrospun silk fibroin/poly(lactide-co-ε-caprolactone) nanofibrous scaffolds for bone regeneration
description Zi Wang,1,* Ming Lin,1,* Qing Xie,1 Hao Sun,1 Yazhuo Huang,1 DanDan Zhang,1 Zhang Yu,1 Xiaoping Bi,1 Junzhao Chen,1 Jing Wang,2 Wodong Shi,1 Ping Gu,1 Xianqun Fan1 1Department of Ophthalmology, Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, 2Biomaterials and Tissue Engineering Laboratory, College of Chemistry & Chemical Engineering and Biotechnology, Donghua University, Shanghai, People’s Republic of China *These authors contributed equally to this work Background: Tissue engineering has become a promising therapeutic approach for bone regeneration. Nanofibrous scaffolds have attracted great interest mainly due to their structural similarity to natural extracellular matrix (ECM). Poly(lactide-co-ε-caprolactone) (PLCL) has been successfully used in bone regeneration, but PLCL polymers are inert and lack natural cell recognition sites, and the surface of PLCL scaffold is hydrophobic. Silk fibroin (SF) is a kind of natural polymer with inherent bioactivity, and supports mesenchymal stem cell attachment, osteogenesis, and ECM deposition. Therefore, we fabricated hybrid nanofibrous scaffolds by adding different weight ratios of SF to PLCL in order to find a scaffold with improved properties for bone regeneration.Methods: Hybrid nanofibrous scaffolds were fabricated by blending different weight ratios of SF with PLCL. Human adipose-derived stem cells (hADSCs) were seeded on SF/PLCL nanofibrous scaffolds of various ratios for a systematic evaluation of cell adhesion, proliferation, cytotoxicity, and osteogenic differentiation; the efficacy of the composite of hADSCs and scaffolds in repairing critical-sized calvarial defects in rats was investigated.Results: The SF/PLCL (50/50) scaffold exhibited favorable tensile strength, surface roughness, and hydrophilicity, which facilitated cell adhesion and proliferation. Moreover, the SF/PLCL (50/50) scaffold promoted the osteogenic differentiation of hADSCs by elevating the expression levels of osteogenic marker genes such as BSP, Ocn, Col1A1, and OPN and enhanced ECM mineralization. In vivo assays showed that SF/PLCL (50/50) scaffold improved the repair of the critical-sized calvarial defect in rats, resulting in increased bone volume, higher trabecular number, enhanced bone mineral density, and increased new bone areas, compared with the pure PLCL scaffold.Conclusion: The SF/PLCL (50/50) nanofibrous scaffold facilitated hADSC proliferation and osteogenic differentiation in vitro and further promoted new bone formation in vivo, suggesting that the SF/PLCL (50/50) nanofibrous scaffold holds great potential in bone tissue regeneration. Keywords: human adipose-derived mesenchymal stem cells, silk fibrin, poly(lactide-co-ε-caprolactone), proliferation, osteogenesis
format article
author Wang Z
Lin M
Xie Q
Sun H
Huang Y
Zhang DD
Yu Z
Bi X
Chen J
Wang J
Shi W
Gu P
Fan X
author_facet Wang Z
Lin M
Xie Q
Sun H
Huang Y
Zhang DD
Yu Z
Bi X
Chen J
Wang J
Shi W
Gu P
Fan X
author_sort Wang Z
title Electrospun silk fibroin/poly(lactide-co-ε-caprolactone) nanofibrous scaffolds for bone regeneration
title_short Electrospun silk fibroin/poly(lactide-co-ε-caprolactone) nanofibrous scaffolds for bone regeneration
title_full Electrospun silk fibroin/poly(lactide-co-ε-caprolactone) nanofibrous scaffolds for bone regeneration
title_fullStr Electrospun silk fibroin/poly(lactide-co-ε-caprolactone) nanofibrous scaffolds for bone regeneration
title_full_unstemmed Electrospun silk fibroin/poly(lactide-co-ε-caprolactone) nanofibrous scaffolds for bone regeneration
title_sort electrospun silk fibroin/poly(lactide-co-ε-caprolactone) nanofibrous scaffolds for bone regeneration
publisher Dove Medical Press
publishDate 2016
url https://doaj.org/article/9e7d56de88974ceeac98e3a4f6684ec3
work_keys_str_mv AT wangz electrospunsilkfibroinpolylactidecoepsiloncaprolactonenanofibrousscaffoldsforboneregeneration
AT linm electrospunsilkfibroinpolylactidecoepsiloncaprolactonenanofibrousscaffoldsforboneregeneration
AT xieq electrospunsilkfibroinpolylactidecoepsiloncaprolactonenanofibrousscaffoldsforboneregeneration
AT sunh electrospunsilkfibroinpolylactidecoepsiloncaprolactonenanofibrousscaffoldsforboneregeneration
AT huangy electrospunsilkfibroinpolylactidecoepsiloncaprolactonenanofibrousscaffoldsforboneregeneration
AT zhangdd electrospunsilkfibroinpolylactidecoepsiloncaprolactonenanofibrousscaffoldsforboneregeneration
AT yuz electrospunsilkfibroinpolylactidecoepsiloncaprolactonenanofibrousscaffoldsforboneregeneration
AT bix electrospunsilkfibroinpolylactidecoepsiloncaprolactonenanofibrousscaffoldsforboneregeneration
AT chenj electrospunsilkfibroinpolylactidecoepsiloncaprolactonenanofibrousscaffoldsforboneregeneration
AT wangj electrospunsilkfibroinpolylactidecoepsiloncaprolactonenanofibrousscaffoldsforboneregeneration
AT shiw electrospunsilkfibroinpolylactidecoepsiloncaprolactonenanofibrousscaffoldsforboneregeneration
AT gup electrospunsilkfibroinpolylactidecoepsiloncaprolactonenanofibrousscaffoldsforboneregeneration
AT fanx electrospunsilkfibroinpolylactidecoepsiloncaprolactonenanofibrousscaffoldsforboneregeneration
_version_ 1718402664046264320

Ejemplares similares