PHENOTYPIC FEATURES AND CHEMILUMINESCENT ACTIVITY OF NEUTROPHILIC GRANULOCYTES IN THE PATIENTS WITH RENAL CANCER

The aim of our study was to investigate the relationship between phenotype and «respiratory burst» activity of neutrophilic granulocytes in patients with renal cancer. The study included 73 patients with renal cell carcinoma (T3N0M0, clear-cell type) before surgical treatment at the age of 40-55 yea...

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Autores principales:	A. A. Savchenko, A. G. Borisov, A. A. Modestov, I. V. Kudryavtsev, A. V. Moshev, I. I. Gvozdev, O. G. Tonacheva
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2016
Materias:	renal cancer neutrophils phenotype functional activity activation markers adhesion receptors respiratory burst Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/9e7e11df227d4142b33d965c9d4dce04
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spelling	oai:doaj.org-article:9e7e11df227d4142b33d965c9d4dce042021-11-18T08:03:45ZPHENOTYPIC FEATURES AND CHEMILUMINESCENT ACTIVITY OF NEUTROPHILIC GRANULOCYTES IN THE PATIENTS WITH RENAL CANCER1563-06252313-741X10.15789/1563-0625-2016-3-259-268https://doaj.org/article/9e7e11df227d4142b33d965c9d4dce042016-07-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/1038https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XThe aim of our study was to investigate the relationship between phenotype and «respiratory burst» activity of neutrophilic granulocytes in patients with renal cancer. The study included 73 patients with renal cell carcinoma (T3N0M0, clear-cell type) before surgical treatment at the age of 40-55 years. The diagnosis of renal cancer was histologically verified in all the patients. Fifty healthy age-matched persons were involved as a control group. Phenotyping of blood neutrophilic granulocytes was performed by flow cytometry. The respiratory burst in blood neutrophilic granulocytes was evaluated using spontaneous and zymosan-induced luminol- and lucigenin-dependent chemiluminescence. Some changes in relationships between the phenotypic indicators of neutrophils and «respiratory burst» were detected in patients with renal cancer. The patients exhibited a loss of relationship between induction of primary and secondary reactive oxygen species, and numbers of CD11b+, CD62L+, and CD64+ neutrophils. These correlations are detected with HLA-DR+ cell levels, like in control group. The patients’ samples showed a correlation between the superoxide radical synthesis by resting neutrophils, and CD62L expression. These relationships disappear when assessing antigen-induced «respiratory burst» response. The correlations between primary and secondary reactive oxygen species, and the numbers of CD11b+, CD62L+ and CD64+ neutrophils found in healthy people characterize a positive relationship between the «respiratory burst» indexes and activation of cells as reflected by expression of adhesive and functional receptors. It may be assumed that altered relationship between the «respiratory burst» indicators and neutrophil phenotype in the patients with renal cell carcinoma is determined by various factors. Firstly, cancer-associated phenotypic changes of blood neutrophils (decreased content of CD11b+, CD62L+, CD64+, HLA-DR+, and HLA-DR+ CD64+ neutrophils, increased expression of CD16, and HLA-DR receptors) thus, possibly, being connected with intra-tumor migration of the cells. Secondly, the patients show an increase in «respiratory burst» intensity in neutrophils, due to enhanced synthesis of primary and secondary reactive oxygen species, which may be connected to the action of various tumor factors (antigens, regulatory molecules).The relationships revealed between phenotype and «respiratory burst» of neutrophils may specifically characterize immunopathogenesis of kidney cancer and can be used in development of immunotherapeutic approaches aiming to stimulate anti-tumor activity of phagocytic cells.A. A. SavchenkoA. G. BorisovA. A. ModestovI. V. KudryavtsevA. V. MoshevI. I. GvozdevO. G. TonachevaSPb RAACIarticlerenal cancerneutrophilsphenotypefunctional activityactivation markersadhesion receptorsrespiratory burstImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 18, Iss 3, Pp 259-268 (2016)
institution	DOAJ
collection	DOAJ
language	RU
topic	renal cancer neutrophils phenotype functional activity activation markers adhesion receptors respiratory burst Immunologic diseases. Allergy RC581-607
spellingShingle	renal cancer neutrophils phenotype functional activity activation markers adhesion receptors respiratory burst Immunologic diseases. Allergy RC581-607 A. A. Savchenko A. G. Borisov A. A. Modestov I. V. Kudryavtsev A. V. Moshev I. I. Gvozdev O. G. Tonacheva PHENOTYPIC FEATURES AND CHEMILUMINESCENT ACTIVITY OF NEUTROPHILIC GRANULOCYTES IN THE PATIENTS WITH RENAL CANCER
description	The aim of our study was to investigate the relationship between phenotype and «respiratory burst» activity of neutrophilic granulocytes in patients with renal cancer. The study included 73 patients with renal cell carcinoma (T3N0M0, clear-cell type) before surgical treatment at the age of 40-55 years. The diagnosis of renal cancer was histologically verified in all the patients. Fifty healthy age-matched persons were involved as a control group. Phenotyping of blood neutrophilic granulocytes was performed by flow cytometry. The respiratory burst in blood neutrophilic granulocytes was evaluated using spontaneous and zymosan-induced luminol- and lucigenin-dependent chemiluminescence. Some changes in relationships between the phenotypic indicators of neutrophils and «respiratory burst» were detected in patients with renal cancer. The patients exhibited a loss of relationship between induction of primary and secondary reactive oxygen species, and numbers of CD11b+, CD62L+, and CD64+ neutrophils. These correlations are detected with HLA-DR+ cell levels, like in control group. The patients’ samples showed a correlation between the superoxide radical synthesis by resting neutrophils, and CD62L expression. These relationships disappear when assessing antigen-induced «respiratory burst» response. The correlations between primary and secondary reactive oxygen species, and the numbers of CD11b+, CD62L+ and CD64+ neutrophils found in healthy people characterize a positive relationship between the «respiratory burst» indexes and activation of cells as reflected by expression of adhesive and functional receptors. It may be assumed that altered relationship between the «respiratory burst» indicators and neutrophil phenotype in the patients with renal cell carcinoma is determined by various factors. Firstly, cancer-associated phenotypic changes of blood neutrophils (decreased content of CD11b+, CD62L+, CD64+, HLA-DR+, and HLA-DR+ CD64+ neutrophils, increased expression of CD16, and HLA-DR receptors) thus, possibly, being connected with intra-tumor migration of the cells. Secondly, the patients show an increase in «respiratory burst» intensity in neutrophils, due to enhanced synthesis of primary and secondary reactive oxygen species, which may be connected to the action of various tumor factors (antigens, regulatory molecules).The relationships revealed between phenotype and «respiratory burst» of neutrophils may specifically characterize immunopathogenesis of kidney cancer and can be used in development of immunotherapeutic approaches aiming to stimulate anti-tumor activity of phagocytic cells.
format	article
author	A. A. Savchenko A. G. Borisov A. A. Modestov I. V. Kudryavtsev A. V. Moshev I. I. Gvozdev O. G. Tonacheva
author_facet	A. A. Savchenko A. G. Borisov A. A. Modestov I. V. Kudryavtsev A. V. Moshev I. I. Gvozdev O. G. Tonacheva
author_sort	A. A. Savchenko
title	PHENOTYPIC FEATURES AND CHEMILUMINESCENT ACTIVITY OF NEUTROPHILIC GRANULOCYTES IN THE PATIENTS WITH RENAL CANCER
title_short	PHENOTYPIC FEATURES AND CHEMILUMINESCENT ACTIVITY OF NEUTROPHILIC GRANULOCYTES IN THE PATIENTS WITH RENAL CANCER
title_full	PHENOTYPIC FEATURES AND CHEMILUMINESCENT ACTIVITY OF NEUTROPHILIC GRANULOCYTES IN THE PATIENTS WITH RENAL CANCER
title_fullStr	PHENOTYPIC FEATURES AND CHEMILUMINESCENT ACTIVITY OF NEUTROPHILIC GRANULOCYTES IN THE PATIENTS WITH RENAL CANCER
title_full_unstemmed	PHENOTYPIC FEATURES AND CHEMILUMINESCENT ACTIVITY OF NEUTROPHILIC GRANULOCYTES IN THE PATIENTS WITH RENAL CANCER
title_sort	phenotypic features and chemiluminescent activity of neutrophilic granulocytes in the patients with renal cancer
publisher	SPb RAACI
publishDate	2016
url	https://doaj.org/article/9e7e11df227d4142b33d965c9d4dce04
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PHENOTYPIC FEATURES AND CHEMILUMINESCENT ACTIVITY OF NEUTROPHILIC GRANULOCYTES IN THE PATIENTS WITH RENAL CANCER

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