Juvenile hormone biosynthesis gene expression in the corpora allata of honey bee (Apis mellifera L.) female castes.
Juvenile hormone (JH) controls key events in the honey bee life cycle, viz. caste development and age polyethism. We quantified transcript abundance of 24 genes involved in the JH biosynthetic pathway in the corpora allata-corpora cardiaca (CA-CC) complex. The expression of six of these genes showin...
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oai:doaj.org-article:9e840688840947ecbb52d21b078680fe2021-11-18T08:35:07ZJuvenile hormone biosynthesis gene expression in the corpora allata of honey bee (Apis mellifera L.) female castes.1932-620310.1371/journal.pone.0086923https://doaj.org/article/9e840688840947ecbb52d21b078680fe2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24489805/?tool=EBIhttps://doaj.org/toc/1932-6203Juvenile hormone (JH) controls key events in the honey bee life cycle, viz. caste development and age polyethism. We quantified transcript abundance of 24 genes involved in the JH biosynthetic pathway in the corpora allata-corpora cardiaca (CA-CC) complex. The expression of six of these genes showing relatively high transcript abundance was contrasted with CA size, hemolymph JH titer, as well as JH degradation rates and JH esterase (jhe) transcript levels. Gene expression did not match the contrasting JH titers in queen and worker fourth instar larvae, but jhe transcript abundance and JH degradation rates were significantly lower in queen larvae. Consequently, transcriptional control of JHE is of importance in regulating larval JH titers and caste development. In contrast, the same analyses applied to adult worker bees allowed us inferring that the high JH levels in foragers are due to increased JH synthesis. Upon RNAi-mediated silencing of the methyl farnesoate epoxidase gene (mfe) encoding the enzyme that catalyzes methyl farnesoate-to-JH conversion, the JH titer was decreased, thus corroborating that JH titer regulation in adult honey bees depends on this final JH biosynthesis step. The molecular pathway differences underlying JH titer regulation in larval caste development versus adult age polyethism lead us to propose that mfe and jhe genes be assayed when addressing questions on the role(s) of JH in social evolution.Ana Durvalina BomtorinAline MackertGustavo Conrado Couto RosaLivia Maria ModaJuliana Ramos MartinsMárcia Maria Gentile BitondiKlaus HartfelderZilá Luz Paulino SimõesPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 1, p e86923 (2014) |
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Medicine R Science Q Ana Durvalina Bomtorin Aline Mackert Gustavo Conrado Couto Rosa Livia Maria Moda Juliana Ramos Martins Márcia Maria Gentile Bitondi Klaus Hartfelder Zilá Luz Paulino Simões Juvenile hormone biosynthesis gene expression in the corpora allata of honey bee (Apis mellifera L.) female castes. |
description |
Juvenile hormone (JH) controls key events in the honey bee life cycle, viz. caste development and age polyethism. We quantified transcript abundance of 24 genes involved in the JH biosynthetic pathway in the corpora allata-corpora cardiaca (CA-CC) complex. The expression of six of these genes showing relatively high transcript abundance was contrasted with CA size, hemolymph JH titer, as well as JH degradation rates and JH esterase (jhe) transcript levels. Gene expression did not match the contrasting JH titers in queen and worker fourth instar larvae, but jhe transcript abundance and JH degradation rates were significantly lower in queen larvae. Consequently, transcriptional control of JHE is of importance in regulating larval JH titers and caste development. In contrast, the same analyses applied to adult worker bees allowed us inferring that the high JH levels in foragers are due to increased JH synthesis. Upon RNAi-mediated silencing of the methyl farnesoate epoxidase gene (mfe) encoding the enzyme that catalyzes methyl farnesoate-to-JH conversion, the JH titer was decreased, thus corroborating that JH titer regulation in adult honey bees depends on this final JH biosynthesis step. The molecular pathway differences underlying JH titer regulation in larval caste development versus adult age polyethism lead us to propose that mfe and jhe genes be assayed when addressing questions on the role(s) of JH in social evolution. |
format |
article |
author |
Ana Durvalina Bomtorin Aline Mackert Gustavo Conrado Couto Rosa Livia Maria Moda Juliana Ramos Martins Márcia Maria Gentile Bitondi Klaus Hartfelder Zilá Luz Paulino Simões |
author_facet |
Ana Durvalina Bomtorin Aline Mackert Gustavo Conrado Couto Rosa Livia Maria Moda Juliana Ramos Martins Márcia Maria Gentile Bitondi Klaus Hartfelder Zilá Luz Paulino Simões |
author_sort |
Ana Durvalina Bomtorin |
title |
Juvenile hormone biosynthesis gene expression in the corpora allata of honey bee (Apis mellifera L.) female castes. |
title_short |
Juvenile hormone biosynthesis gene expression in the corpora allata of honey bee (Apis mellifera L.) female castes. |
title_full |
Juvenile hormone biosynthesis gene expression in the corpora allata of honey bee (Apis mellifera L.) female castes. |
title_fullStr |
Juvenile hormone biosynthesis gene expression in the corpora allata of honey bee (Apis mellifera L.) female castes. |
title_full_unstemmed |
Juvenile hormone biosynthesis gene expression in the corpora allata of honey bee (Apis mellifera L.) female castes. |
title_sort |
juvenile hormone biosynthesis gene expression in the corpora allata of honey bee (apis mellifera l.) female castes. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2014 |
url |
https://doaj.org/article/9e840688840947ecbb52d21b078680fe |
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