Juvenile hormone biosynthesis gene expression in the corpora allata of honey bee (Apis mellifera L.) female castes.

Juvenile hormone (JH) controls key events in the honey bee life cycle, viz. caste development and age polyethism. We quantified transcript abundance of 24 genes involved in the JH biosynthetic pathway in the corpora allata-corpora cardiaca (CA-CC) complex. The expression of six of these genes showin...

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Autores principales:	Ana Durvalina Bomtorin, Aline Mackert, Gustavo Conrado Couto Rosa, Livia Maria Moda, Juliana Ramos Martins, Márcia Maria Gentile Bitondi, Klaus Hartfelder, Zilá Luz Paulino Simões
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9e840688840947ecbb52d21b078680fe
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spelling	oai:doaj.org-article:9e840688840947ecbb52d21b078680fe2021-11-18T08:35:07ZJuvenile hormone biosynthesis gene expression in the corpora allata of honey bee (Apis mellifera L.) female castes.1932-620310.1371/journal.pone.0086923https://doaj.org/article/9e840688840947ecbb52d21b078680fe2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24489805/?tool=EBIhttps://doaj.org/toc/1932-6203Juvenile hormone (JH) controls key events in the honey bee life cycle, viz. caste development and age polyethism. We quantified transcript abundance of 24 genes involved in the JH biosynthetic pathway in the corpora allata-corpora cardiaca (CA-CC) complex. The expression of six of these genes showing relatively high transcript abundance was contrasted with CA size, hemolymph JH titer, as well as JH degradation rates and JH esterase (jhe) transcript levels. Gene expression did not match the contrasting JH titers in queen and worker fourth instar larvae, but jhe transcript abundance and JH degradation rates were significantly lower in queen larvae. Consequently, transcriptional control of JHE is of importance in regulating larval JH titers and caste development. In contrast, the same analyses applied to adult worker bees allowed us inferring that the high JH levels in foragers are due to increased JH synthesis. Upon RNAi-mediated silencing of the methyl farnesoate epoxidase gene (mfe) encoding the enzyme that catalyzes methyl farnesoate-to-JH conversion, the JH titer was decreased, thus corroborating that JH titer regulation in adult honey bees depends on this final JH biosynthesis step. The molecular pathway differences underlying JH titer regulation in larval caste development versus adult age polyethism lead us to propose that mfe and jhe genes be assayed when addressing questions on the role(s) of JH in social evolution.Ana Durvalina BomtorinAline MackertGustavo Conrado Couto RosaLivia Maria ModaJuliana Ramos MartinsMárcia Maria Gentile BitondiKlaus HartfelderZilá Luz Paulino SimõesPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 1, p e86923 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ana Durvalina Bomtorin Aline Mackert Gustavo Conrado Couto Rosa Livia Maria Moda Juliana Ramos Martins Márcia Maria Gentile Bitondi Klaus Hartfelder Zilá Luz Paulino Simões Juvenile hormone biosynthesis gene expression in the corpora allata of honey bee (Apis mellifera L.) female castes.
description	Juvenile hormone (JH) controls key events in the honey bee life cycle, viz. caste development and age polyethism. We quantified transcript abundance of 24 genes involved in the JH biosynthetic pathway in the corpora allata-corpora cardiaca (CA-CC) complex. The expression of six of these genes showing relatively high transcript abundance was contrasted with CA size, hemolymph JH titer, as well as JH degradation rates and JH esterase (jhe) transcript levels. Gene expression did not match the contrasting JH titers in queen and worker fourth instar larvae, but jhe transcript abundance and JH degradation rates were significantly lower in queen larvae. Consequently, transcriptional control of JHE is of importance in regulating larval JH titers and caste development. In contrast, the same analyses applied to adult worker bees allowed us inferring that the high JH levels in foragers are due to increased JH synthesis. Upon RNAi-mediated silencing of the methyl farnesoate epoxidase gene (mfe) encoding the enzyme that catalyzes methyl farnesoate-to-JH conversion, the JH titer was decreased, thus corroborating that JH titer regulation in adult honey bees depends on this final JH biosynthesis step. The molecular pathway differences underlying JH titer regulation in larval caste development versus adult age polyethism lead us to propose that mfe and jhe genes be assayed when addressing questions on the role(s) of JH in social evolution.
format	article
author	Ana Durvalina Bomtorin Aline Mackert Gustavo Conrado Couto Rosa Livia Maria Moda Juliana Ramos Martins Márcia Maria Gentile Bitondi Klaus Hartfelder Zilá Luz Paulino Simões
author_facet	Ana Durvalina Bomtorin Aline Mackert Gustavo Conrado Couto Rosa Livia Maria Moda Juliana Ramos Martins Márcia Maria Gentile Bitondi Klaus Hartfelder Zilá Luz Paulino Simões
author_sort	Ana Durvalina Bomtorin
title	Juvenile hormone biosynthesis gene expression in the corpora allata of honey bee (Apis mellifera L.) female castes.
title_short	Juvenile hormone biosynthesis gene expression in the corpora allata of honey bee (Apis mellifera L.) female castes.
title_full	Juvenile hormone biosynthesis gene expression in the corpora allata of honey bee (Apis mellifera L.) female castes.
title_fullStr	Juvenile hormone biosynthesis gene expression in the corpora allata of honey bee (Apis mellifera L.) female castes.
title_full_unstemmed	Juvenile hormone biosynthesis gene expression in the corpora allata of honey bee (Apis mellifera L.) female castes.
title_sort	juvenile hormone biosynthesis gene expression in the corpora allata of honey bee (apis mellifera l.) female castes.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/9e840688840947ecbb52d21b078680fe
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Juvenile hormone biosynthesis gene expression in the corpora allata of honey bee (Apis mellifera L.) female castes.

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