The Role of TRPC6 in the Neuroprotection of Calycosin Against Cerebral Ischemic Injury

Abstract Our previous studies have provided evidences that calycosin can protect the brain from ischemia/reperfusion injury, but its mechanisms is not fully understand. Transient receptor potential canonical 6 (TRPC6) has a critical role in promoting neuronal survival against cerebral ischemic injur...

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Autores principales:	Chao Guo, Yongyuan Ma, Shanbo Ma, Fei Mu, Jiao Deng, Jialin Duan, Lize Xiong, Ying Yin, Yanhua Wang, Miaomaio Xi, Aidong Wen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9e842cf42f17499a897ccda94624e443
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spelling	oai:doaj.org-article:9e842cf42f17499a897ccda94624e4432021-12-02T16:06:46ZThe Role of TRPC6 in the Neuroprotection of Calycosin Against Cerebral Ischemic Injury10.1038/s41598-017-03404-62045-2322https://doaj.org/article/9e842cf42f17499a897ccda94624e4432017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03404-6https://doaj.org/toc/2045-2322Abstract Our previous studies have provided evidences that calycosin can protect the brain from ischemia/reperfusion injury, but its mechanisms is not fully understand. Transient receptor potential canonical 6 (TRPC6) has a critical role in promoting neuronal survival against cerebral ischemic injury. The aim of the present study is to test whether calycosin protects against cerebral ischemic injury through TRPC6-CREB pathway. In vivo, rats were subjected to transient middle cerebral artery occlusion (MCAO) for 2 h and then treated with different doses of calycosin at the onset of reperfusion. In vitro, primary cultured neurons were treated by calycosin, then exposed to 2 h oxygen glucose deprivation (OGD) followed by 24 h reoxygenation. Our results showed that treatment with calycosin protected against ischemia-induced damages by increasing TRPC6 and P-CREB expression and inhibiting calpain activation. The neuroprotection effect of calycosin was diminished by inhibition or knockdown of TRPC6 and CREB. These findings indicated that the potential neuroprotection mechanism of calycosin was involved with TRPC6-CREB pathway.Chao GuoYongyuan MaShanbo MaFei MuJiao DengJialin DuanLize XiongYing YinYanhua WangMiaomaio XiAidong WenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Chao Guo Yongyuan Ma Shanbo Ma Fei Mu Jiao Deng Jialin Duan Lize Xiong Ying Yin Yanhua Wang Miaomaio Xi Aidong Wen The Role of TRPC6 in the Neuroprotection of Calycosin Against Cerebral Ischemic Injury
description	Abstract Our previous studies have provided evidences that calycosin can protect the brain from ischemia/reperfusion injury, but its mechanisms is not fully understand. Transient receptor potential canonical 6 (TRPC6) has a critical role in promoting neuronal survival against cerebral ischemic injury. The aim of the present study is to test whether calycosin protects against cerebral ischemic injury through TRPC6-CREB pathway. In vivo, rats were subjected to transient middle cerebral artery occlusion (MCAO) for 2 h and then treated with different doses of calycosin at the onset of reperfusion. In vitro, primary cultured neurons were treated by calycosin, then exposed to 2 h oxygen glucose deprivation (OGD) followed by 24 h reoxygenation. Our results showed that treatment with calycosin protected against ischemia-induced damages by increasing TRPC6 and P-CREB expression and inhibiting calpain activation. The neuroprotection effect of calycosin was diminished by inhibition or knockdown of TRPC6 and CREB. These findings indicated that the potential neuroprotection mechanism of calycosin was involved with TRPC6-CREB pathway.
format	article
author	Chao Guo Yongyuan Ma Shanbo Ma Fei Mu Jiao Deng Jialin Duan Lize Xiong Ying Yin Yanhua Wang Miaomaio Xi Aidong Wen
author_facet	Chao Guo Yongyuan Ma Shanbo Ma Fei Mu Jiao Deng Jialin Duan Lize Xiong Ying Yin Yanhua Wang Miaomaio Xi Aidong Wen
author_sort	Chao Guo
title	The Role of TRPC6 in the Neuroprotection of Calycosin Against Cerebral Ischemic Injury
title_short	The Role of TRPC6 in the Neuroprotection of Calycosin Against Cerebral Ischemic Injury
title_full	The Role of TRPC6 in the Neuroprotection of Calycosin Against Cerebral Ischemic Injury
title_fullStr	The Role of TRPC6 in the Neuroprotection of Calycosin Against Cerebral Ischemic Injury
title_full_unstemmed	The Role of TRPC6 in the Neuroprotection of Calycosin Against Cerebral Ischemic Injury
title_sort	role of trpc6 in the neuroprotection of calycosin against cerebral ischemic injury
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/9e842cf42f17499a897ccda94624e443
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The Role of TRPC6 in the Neuroprotection of Calycosin Against Cerebral Ischemic Injury

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