Rapid construction of a whole-genome transposon insertion collection for Shewanella oneidensis by Knockout Sudoku

Knockout collections provide a valuable tool to explore gene function, yet are expensive and technically challenging to produce at a genome-wide scale. Here Baym et al. devise a cost-effective transposon-based method to quickly develop a knockout collection for the electroactive microbe Shewanella o...

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Autores principales: Michael Baym, Lev Shaket, Isao A. Anzai, Oluwakemi Adesina, Buz Barstow
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2016
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Acceso en línea:https://doaj.org/article/9e8559f47b0a41eea7bc0f3f5d00ce9b
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Sumario:Knockout collections provide a valuable tool to explore gene function, yet are expensive and technically challenging to produce at a genome-wide scale. Here Baym et al. devise a cost-effective transposon-based method to quickly develop a knockout collection for the electroactive microbe Shewanella oneidensis.