Rapid construction of a whole-genome transposon insertion collection for Shewanella oneidensis by Knockout Sudoku

Knockout collections provide a valuable tool to explore gene function, yet are expensive and technically challenging to produce at a genome-wide scale. Here Baym et al. devise a cost-effective transposon-based method to quickly develop a knockout collection for the electroactive microbe Shewanella o...

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Autores principales:	Michael Baym, Lev Shaket, Isao A. Anzai, Oluwakemi Adesina, Buz Barstow
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2016
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/9e8559f47b0a41eea7bc0f3f5d00ce9b
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spelling	oai:doaj.org-article:9e8559f47b0a41eea7bc0f3f5d00ce9b2021-12-02T17:32:35ZRapid construction of a whole-genome transposon insertion collection for Shewanella oneidensis by Knockout Sudoku10.1038/ncomms132702041-1723https://doaj.org/article/9e8559f47b0a41eea7bc0f3f5d00ce9b2016-11-01T00:00:00Zhttps://doi.org/10.1038/ncomms13270https://doaj.org/toc/2041-1723Knockout collections provide a valuable tool to explore gene function, yet are expensive and technically challenging to produce at a genome-wide scale. Here Baym et al. devise a cost-effective transposon-based method to quickly develop a knockout collection for the electroactive microbe Shewanella oneidensis.Michael BaymLev ShaketIsao A. AnzaiOluwakemi AdesinaBuz BarstowNature PortfolioarticleScienceQENNature Communications, Vol 7, Iss 1, Pp 1-13 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Michael Baym Lev Shaket Isao A. Anzai Oluwakemi Adesina Buz Barstow Rapid construction of a whole-genome transposon insertion collection for Shewanella oneidensis by Knockout Sudoku
description	Knockout collections provide a valuable tool to explore gene function, yet are expensive and technically challenging to produce at a genome-wide scale. Here Baym et al. devise a cost-effective transposon-based method to quickly develop a knockout collection for the electroactive microbe Shewanella oneidensis.
format	article
author	Michael Baym Lev Shaket Isao A. Anzai Oluwakemi Adesina Buz Barstow
author_facet	Michael Baym Lev Shaket Isao A. Anzai Oluwakemi Adesina Buz Barstow
author_sort	Michael Baym
title	Rapid construction of a whole-genome transposon insertion collection for Shewanella oneidensis by Knockout Sudoku
title_short	Rapid construction of a whole-genome transposon insertion collection for Shewanella oneidensis by Knockout Sudoku
title_full	Rapid construction of a whole-genome transposon insertion collection for Shewanella oneidensis by Knockout Sudoku
title_fullStr	Rapid construction of a whole-genome transposon insertion collection for Shewanella oneidensis by Knockout Sudoku
title_full_unstemmed	Rapid construction of a whole-genome transposon insertion collection for Shewanella oneidensis by Knockout Sudoku
title_sort	rapid construction of a whole-genome transposon insertion collection for shewanella oneidensis by knockout sudoku
publisher	Nature Portfolio
publishDate	2016
url	https://doaj.org/article/9e8559f47b0a41eea7bc0f3f5d00ce9b
work_keys_str_mv	AT michaelbaym rapidconstructionofawholegenometransposoninsertioncollectionforshewanellaoneidensisbyknockoutsudoku AT levshaket rapidconstructionofawholegenometransposoninsertioncollectionforshewanellaoneidensisbyknockoutsudoku AT isaoaanzai rapidconstructionofawholegenometransposoninsertioncollectionforshewanellaoneidensisbyknockoutsudoku AT oluwakemiadesina rapidconstructionofawholegenometransposoninsertioncollectionforshewanellaoneidensisbyknockoutsudoku AT buzbarstow rapidconstructionofawholegenometransposoninsertioncollectionforshewanellaoneidensisbyknockoutsudoku
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Rapid construction of a whole-genome transposon insertion collection for Shewanella oneidensis by Knockout Sudoku

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