A novel potential primary method for quantification of enantiomers by high performance liquid chromatography-circular dichroism

Abstract Primary methods play an important role in metrology. They can be used for the value assignment of certified reference materials, enabling the accuracy and comparability of the measurement. A novel potential primary method for enantiomer quantitation based on high-performance liquid chromato...

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Autores principales:	Yi Luo, Liqing Wu, Bin Yang, Youxun Jin, Kangle Zheng, Zhangjing He
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9e8c909a96ff48fea190a3ae40a6f5f4
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spelling	oai:doaj.org-article:9e8c909a96ff48fea190a3ae40a6f5f42021-12-02T15:09:10ZA novel potential primary method for quantification of enantiomers by high performance liquid chromatography-circular dichroism10.1038/s41598-018-25682-42045-2322https://doaj.org/article/9e8c909a96ff48fea190a3ae40a6f5f42018-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-25682-4https://doaj.org/toc/2045-2322Abstract Primary methods play an important role in metrology. They can be used for the value assignment of certified reference materials, enabling the accuracy and comparability of the measurement. A novel potential primary method for enantiomer quantitation based on high-performance liquid chromatography-circular dichroism is described using L-phenylalanine as an example. The optimal quantitation range of L-Phe was from 0.1 mg/g to 1.2 mg/g, where both the relative bias and method variance were lower than 1%. The LOD and LOQ were 4 μg/g and 30 μg/g, respectively. The proposed method was also applied to the determination of the mass fraction of pure porcine insulin in solid. The average mass fraction obtained was 0.922 g/g with a RSD of 1.5%, and the associated relative uncertainty is 3.8% (k = 2), which agreed well with that obtained from the traditional isotope dilution mass spectrometry method. The LOD and LOQ for insulin quantitation were found to be 0.12 mg/g and 0.44 mg/g, respectively. The proposed method can be entirely described and understood by equations and a complete uncertainty statement can be defined in SI units.Therefore, it may be a potential primary method useful for the quantification of chiral compounds and proteins, and a supplementary method to the traditional isotope dilution mass spectrometry approach.Yi LuoLiqing WuBin YangYouxun JinKangle ZhengZhangjing HeNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-11 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yi Luo Liqing Wu Bin Yang Youxun Jin Kangle Zheng Zhangjing He A novel potential primary method for quantification of enantiomers by high performance liquid chromatography-circular dichroism
description	Abstract Primary methods play an important role in metrology. They can be used for the value assignment of certified reference materials, enabling the accuracy and comparability of the measurement. A novel potential primary method for enantiomer quantitation based on high-performance liquid chromatography-circular dichroism is described using L-phenylalanine as an example. The optimal quantitation range of L-Phe was from 0.1 mg/g to 1.2 mg/g, where both the relative bias and method variance were lower than 1%. The LOD and LOQ were 4 μg/g and 30 μg/g, respectively. The proposed method was also applied to the determination of the mass fraction of pure porcine insulin in solid. The average mass fraction obtained was 0.922 g/g with a RSD of 1.5%, and the associated relative uncertainty is 3.8% (k = 2), which agreed well with that obtained from the traditional isotope dilution mass spectrometry method. The LOD and LOQ for insulin quantitation were found to be 0.12 mg/g and 0.44 mg/g, respectively. The proposed method can be entirely described and understood by equations and a complete uncertainty statement can be defined in SI units.Therefore, it may be a potential primary method useful for the quantification of chiral compounds and proteins, and a supplementary method to the traditional isotope dilution mass spectrometry approach.
format	article
author	Yi Luo Liqing Wu Bin Yang Youxun Jin Kangle Zheng Zhangjing He
author_facet	Yi Luo Liqing Wu Bin Yang Youxun Jin Kangle Zheng Zhangjing He
author_sort	Yi Luo
title	A novel potential primary method for quantification of enantiomers by high performance liquid chromatography-circular dichroism
title_short	A novel potential primary method for quantification of enantiomers by high performance liquid chromatography-circular dichroism
title_full	A novel potential primary method for quantification of enantiomers by high performance liquid chromatography-circular dichroism
title_fullStr	A novel potential primary method for quantification of enantiomers by high performance liquid chromatography-circular dichroism
title_full_unstemmed	A novel potential primary method for quantification of enantiomers by high performance liquid chromatography-circular dichroism
title_sort	novel potential primary method for quantification of enantiomers by high performance liquid chromatography-circular dichroism
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/9e8c909a96ff48fea190a3ae40a6f5f4
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A novel potential primary method for quantification of enantiomers by high performance liquid chromatography-circular dichroism

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