Sulforaphane induces S-phase arrest and apoptosis via p53-dependent manner in gastric cancer cells

Abstract Sulforaphane (SFN) extracted from broccoli sprout has previously been investigated for its potential properties in cancers, however, the underlying mechanisms of the anticancer activity of SFN remain not fully understood. In the present study, we investigate the effects of SFN on cell proli...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Yuan Wang, Huazhang Wu, Nannan Dong, Xu Su, Mingxiu Duan, Yaqin Wei, Jun Wei, Gaofeng Liu, Qingjie Peng, Yunli Zhao
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9e901890976f4abebe1bc0cef4f9bfae
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:9e901890976f4abebe1bc0cef4f9bfae
record_format	dspace
spelling	oai:doaj.org-article:9e901890976f4abebe1bc0cef4f9bfae2021-12-02T10:48:03ZSulforaphane induces S-phase arrest and apoptosis via p53-dependent manner in gastric cancer cells10.1038/s41598-021-81815-22045-2322https://doaj.org/article/9e901890976f4abebe1bc0cef4f9bfae2021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-81815-2https://doaj.org/toc/2045-2322Abstract Sulforaphane (SFN) extracted from broccoli sprout has previously been investigated for its potential properties in cancers, however, the underlying mechanisms of the anticancer activity of SFN remain not fully understood. In the present study, we investigate the effects of SFN on cell proliferation, cell cycle, cell apoptosis, and also the expression of several cell cycle and apoptosis-related genes by MTT assay, flow cytometry and western blot analysis in gastric cancer (GC) cells. The results showed that SFN could impair the colony-forming ability in BGC-823 and MGC-803 cell lines compared with the control. In addition, SFN significantly suppressed cell proliferation by arresting the cell cycle at the S phase and enhancing cell apoptosis in GC cells in a dose-dependent manner. Western blot results showed that SFN treatment significantly increased the expression levels of p53, p21 and decreased CDK2 expression, which directly regulated the S phase transition. The Bax and cleaved-caspase-3 genes involved in apoptosis executive functions were significantly increased in a dose-dependent manner in BGC-823 and MGC-803 cells. These results suggested that SFN-induced S phase cell cycle arrest and apoptosis through p53-dependent manner in GC cells, which suggested that SFN has a potential therapeutic application in the treatment and prevention of GC.Yuan WangHuazhang WuNannan DongXu SuMingxiu DuanYaqin WeiJun WeiGaofeng LiuQingjie PengYunli ZhaoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yuan Wang Huazhang Wu Nannan Dong Xu Su Mingxiu Duan Yaqin Wei Jun Wei Gaofeng Liu Qingjie Peng Yunli Zhao Sulforaphane induces S-phase arrest and apoptosis via p53-dependent manner in gastric cancer cells
description	Abstract Sulforaphane (SFN) extracted from broccoli sprout has previously been investigated for its potential properties in cancers, however, the underlying mechanisms of the anticancer activity of SFN remain not fully understood. In the present study, we investigate the effects of SFN on cell proliferation, cell cycle, cell apoptosis, and also the expression of several cell cycle and apoptosis-related genes by MTT assay, flow cytometry and western blot analysis in gastric cancer (GC) cells. The results showed that SFN could impair the colony-forming ability in BGC-823 and MGC-803 cell lines compared with the control. In addition, SFN significantly suppressed cell proliferation by arresting the cell cycle at the S phase and enhancing cell apoptosis in GC cells in a dose-dependent manner. Western blot results showed that SFN treatment significantly increased the expression levels of p53, p21 and decreased CDK2 expression, which directly regulated the S phase transition. The Bax and cleaved-caspase-3 genes involved in apoptosis executive functions were significantly increased in a dose-dependent manner in BGC-823 and MGC-803 cells. These results suggested that SFN-induced S phase cell cycle arrest and apoptosis through p53-dependent manner in GC cells, which suggested that SFN has a potential therapeutic application in the treatment and prevention of GC.
format	article
author	Yuan Wang Huazhang Wu Nannan Dong Xu Su Mingxiu Duan Yaqin Wei Jun Wei Gaofeng Liu Qingjie Peng Yunli Zhao
author_facet	Yuan Wang Huazhang Wu Nannan Dong Xu Su Mingxiu Duan Yaqin Wei Jun Wei Gaofeng Liu Qingjie Peng Yunli Zhao
author_sort	Yuan Wang
title	Sulforaphane induces S-phase arrest and apoptosis via p53-dependent manner in gastric cancer cells
title_short	Sulforaphane induces S-phase arrest and apoptosis via p53-dependent manner in gastric cancer cells
title_full	Sulforaphane induces S-phase arrest and apoptosis via p53-dependent manner in gastric cancer cells
title_fullStr	Sulforaphane induces S-phase arrest and apoptosis via p53-dependent manner in gastric cancer cells
title_full_unstemmed	Sulforaphane induces S-phase arrest and apoptosis via p53-dependent manner in gastric cancer cells
title_sort	sulforaphane induces s-phase arrest and apoptosis via p53-dependent manner in gastric cancer cells
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/9e901890976f4abebe1bc0cef4f9bfae
work_keys_str_mv	AT yuanwang sulforaphaneinducessphasearrestandapoptosisviap53dependentmanneringastriccancercells AT huazhangwu sulforaphaneinducessphasearrestandapoptosisviap53dependentmanneringastriccancercells AT nannandong sulforaphaneinducessphasearrestandapoptosisviap53dependentmanneringastriccancercells AT xusu sulforaphaneinducessphasearrestandapoptosisviap53dependentmanneringastriccancercells AT mingxiuduan sulforaphaneinducessphasearrestandapoptosisviap53dependentmanneringastriccancercells AT yaqinwei sulforaphaneinducessphasearrestandapoptosisviap53dependentmanneringastriccancercells AT junwei sulforaphaneinducessphasearrestandapoptosisviap53dependentmanneringastriccancercells AT gaofengliu sulforaphaneinducessphasearrestandapoptosisviap53dependentmanneringastriccancercells AT qingjiepeng sulforaphaneinducessphasearrestandapoptosisviap53dependentmanneringastriccancercells AT yunlizhao sulforaphaneinducessphasearrestandapoptosisviap53dependentmanneringastriccancercells
_version_	1718396664957370368

Sulforaphane induces S-phase arrest and apoptosis via p53-dependent manner in gastric cancer cells

Ejemplares similares