Genomic and Transcriptomic Evidence Supports Methane Metabolism in <italic toggle="yes">Archaeoglobi</italic>

ABSTRACT Euryarchaeal lineages have been believed to have a methanogenic last common ancestor. However, members of euryarchaeal Archaeoglobi have long been considered nonmethanogenic and their evolutionary history remains elusive. Here, three high-quality metagenomic-assembled genomes (MAGs) retriev...

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Autores principales:	Yi-Fan Liu, Jing Chen, Livia S. Zaramela, Li-Ying Wang, Serge Maurice Mbadinga, Zhao-Wei Hou, Xiao-Lin Wu, Ji-Dong Gu, Karsten Zengler, Bo-Zhong Mu
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2020
Materias:	horizontal gene transfer HGT metatranscriptomics methyl-coenzyme M reductase complex MCR complex oil reservoir Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/9e91808439d24706948115d91a8eefe0
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spelling	oai:doaj.org-article:9e91808439d24706948115d91a8eefe02021-12-02T19:46:20ZGenomic and Transcriptomic Evidence Supports Methane Metabolism in <italic toggle="yes">Archaeoglobi</italic>10.1128/mSystems.00651-192379-5077https://doaj.org/article/9e91808439d24706948115d91a8eefe02020-04-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSystems.00651-19https://doaj.org/toc/2379-5077ABSTRACT Euryarchaeal lineages have been believed to have a methanogenic last common ancestor. However, members of euryarchaeal Archaeoglobi have long been considered nonmethanogenic and their evolutionary history remains elusive. Here, three high-quality metagenomic-assembled genomes (MAGs) retrieved from high-temperature oil reservoir and hot springs, together with three newly assembled Archaeoglobi MAGs from previously reported hot spring metagenomes, are demonstrated to represent a novel genus of Archaeoglobaceae, “Candidatus Methanomixophus.” All “Ca. Methanomixophus” MAGs encode an M methyltransferase (MTR) complex and a traditional type of methyl-coenzyme M reductase (MCR) complex, which is different from the divergent MCR complexes found in “Ca. Polytropus marinifundus.” In addition, “Ca. Methanomixophus dualitatem” MAGs preserve the genomic capacity for dissimilatory sulfate reduction. Comparative phylogenetic analysis supports a laterally transferred origin for an MCR complex and vertical heritage of the MTR complex in this lineage. Metatranscriptomic analysis revealed concomitant in situ activity of hydrogen-dependent methylotrophic methanogenesis and heterotrophic fermentation within populations of “Ca. Methanomixophus hydrogenotrophicum” in a high-temperature oil reservoir. IMPORTANCE Current understanding of the diversity, biology, and ecology of Archaea is very limited, especially considering how few of the known phyla have been cultured or genomically explored. The reconstruction of “Ca. Methanomixophus” MAGs not only expands the known range of metabolic versatility of the members of Archaeoglobi but also suggests that the phylogenetic distribution of MCR and MTR complexes is even wider than previously anticipated.Yi-Fan LiuJing ChenLivia S. ZaramelaLi-Ying WangSerge Maurice MbadingaZhao-Wei HouXiao-Lin WuJi-Dong GuKarsten ZenglerBo-Zhong MuAmerican Society for Microbiologyarticlehorizontal gene transferHGTmetatranscriptomicsmethyl-coenzyme M reductase complexMCR complexoil reservoirMicrobiologyQR1-502ENmSystems, Vol 5, Iss 2 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	horizontal gene transfer HGT metatranscriptomics methyl-coenzyme M reductase complex MCR complex oil reservoir Microbiology QR1-502
spellingShingle	horizontal gene transfer HGT metatranscriptomics methyl-coenzyme M reductase complex MCR complex oil reservoir Microbiology QR1-502 Yi-Fan Liu Jing Chen Livia S. Zaramela Li-Ying Wang Serge Maurice Mbadinga Zhao-Wei Hou Xiao-Lin Wu Ji-Dong Gu Karsten Zengler Bo-Zhong Mu Genomic and Transcriptomic Evidence Supports Methane Metabolism in <italic toggle="yes">Archaeoglobi</italic>
description	ABSTRACT Euryarchaeal lineages have been believed to have a methanogenic last common ancestor. However, members of euryarchaeal Archaeoglobi have long been considered nonmethanogenic and their evolutionary history remains elusive. Here, three high-quality metagenomic-assembled genomes (MAGs) retrieved from high-temperature oil reservoir and hot springs, together with three newly assembled Archaeoglobi MAGs from previously reported hot spring metagenomes, are demonstrated to represent a novel genus of Archaeoglobaceae, “Candidatus Methanomixophus.” All “Ca. Methanomixophus” MAGs encode an M methyltransferase (MTR) complex and a traditional type of methyl-coenzyme M reductase (MCR) complex, which is different from the divergent MCR complexes found in “Ca. Polytropus marinifundus.” In addition, “Ca. Methanomixophus dualitatem” MAGs preserve the genomic capacity for dissimilatory sulfate reduction. Comparative phylogenetic analysis supports a laterally transferred origin for an MCR complex and vertical heritage of the MTR complex in this lineage. Metatranscriptomic analysis revealed concomitant in situ activity of hydrogen-dependent methylotrophic methanogenesis and heterotrophic fermentation within populations of “Ca. Methanomixophus hydrogenotrophicum” in a high-temperature oil reservoir. IMPORTANCE Current understanding of the diversity, biology, and ecology of Archaea is very limited, especially considering how few of the known phyla have been cultured or genomically explored. The reconstruction of “Ca. Methanomixophus” MAGs not only expands the known range of metabolic versatility of the members of Archaeoglobi but also suggests that the phylogenetic distribution of MCR and MTR complexes is even wider than previously anticipated.
format	article
author	Yi-Fan Liu Jing Chen Livia S. Zaramela Li-Ying Wang Serge Maurice Mbadinga Zhao-Wei Hou Xiao-Lin Wu Ji-Dong Gu Karsten Zengler Bo-Zhong Mu
author_facet	Yi-Fan Liu Jing Chen Livia S. Zaramela Li-Ying Wang Serge Maurice Mbadinga Zhao-Wei Hou Xiao-Lin Wu Ji-Dong Gu Karsten Zengler Bo-Zhong Mu
author_sort	Yi-Fan Liu
title	Genomic and Transcriptomic Evidence Supports Methane Metabolism in <italic toggle="yes">Archaeoglobi</italic>
title_short	Genomic and Transcriptomic Evidence Supports Methane Metabolism in <italic toggle="yes">Archaeoglobi</italic>
title_full	Genomic and Transcriptomic Evidence Supports Methane Metabolism in <italic toggle="yes">Archaeoglobi</italic>
title_fullStr	Genomic and Transcriptomic Evidence Supports Methane Metabolism in <italic toggle="yes">Archaeoglobi</italic>
title_full_unstemmed	Genomic and Transcriptomic Evidence Supports Methane Metabolism in <italic toggle="yes">Archaeoglobi</italic>
title_sort	genomic and transcriptomic evidence supports methane metabolism in <italic toggle="yes">archaeoglobi</italic>
publisher	American Society for Microbiology
publishDate	2020
url	https://doaj.org/article/9e91808439d24706948115d91a8eefe0
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Genomic and Transcriptomic Evidence Supports Methane Metabolism in <italic toggle="yes">Archaeoglobi</italic>

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