Complete genome sequence and comparative genomics of the probiotic yeast Saccharomyces boulardii

Abstract The probiotic yeast, Saccharomyces boulardii (Sb) is known to be effective against many gastrointestinal disorders and antibiotic-associated diarrhea. To understand molecular basis of probiotic-properties ascribed to Sb we determined the complete genomes of two strains of Sb i.e. Biocodex a...

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Autores principales:	Indu Khatri, Rajul Tomar, K. Ganesan, G. S. Prasad, Srikrishna Subramanian
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9e93e773e2ad4d18ad8274186469255b
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spelling	oai:doaj.org-article:9e93e773e2ad4d18ad8274186469255b2021-12-02T15:06:16ZComplete genome sequence and comparative genomics of the probiotic yeast Saccharomyces boulardii10.1038/s41598-017-00414-22045-2322https://doaj.org/article/9e93e773e2ad4d18ad8274186469255b2017-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00414-2https://doaj.org/toc/2045-2322Abstract The probiotic yeast, Saccharomyces boulardii (Sb) is known to be effective against many gastrointestinal disorders and antibiotic-associated diarrhea. To understand molecular basis of probiotic-properties ascribed to Sb we determined the complete genomes of two strains of Sb i.e. Biocodex and unique28 and the draft genomes for three other Sb strains that are marketed as probiotics in India. We compared these genomes with 145 strains of S. cerevisiae (Sc) to understand genome-level similarities and differences between these yeasts. A distinctive feature of Sb from other Sc is absence of Ty elements Ty1, Ty3, Ty4 and associated LTR. However, we could identify complete Ty2 and Ty5 elements in Sb. The genes for hexose transporters HXT11 and HXT9, and asparagine-utilization are absent in all Sb strains. We find differences in repeat periods and copy numbers of repeats in flocculin genes that are likely related to the differential adhesion of Sb as compared to Sc. Core-proteome based taxonomy places Sb strains along with wine strains of Sc. We find the introgression of five genes from Z. bailii into the chromosome IV of Sb and wine strains of Sc. Intriguingly, genes involved in conferring known probiotic properties to Sb are conserved in most Sc strains.Indu KhatriRajul TomarK. GanesanG. S. PrasadSrikrishna SubramanianNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Indu Khatri Rajul Tomar K. Ganesan G. S. Prasad Srikrishna Subramanian Complete genome sequence and comparative genomics of the probiotic yeast Saccharomyces boulardii
description	Abstract The probiotic yeast, Saccharomyces boulardii (Sb) is known to be effective against many gastrointestinal disorders and antibiotic-associated diarrhea. To understand molecular basis of probiotic-properties ascribed to Sb we determined the complete genomes of two strains of Sb i.e. Biocodex and unique28 and the draft genomes for three other Sb strains that are marketed as probiotics in India. We compared these genomes with 145 strains of S. cerevisiae (Sc) to understand genome-level similarities and differences between these yeasts. A distinctive feature of Sb from other Sc is absence of Ty elements Ty1, Ty3, Ty4 and associated LTR. However, we could identify complete Ty2 and Ty5 elements in Sb. The genes for hexose transporters HXT11 and HXT9, and asparagine-utilization are absent in all Sb strains. We find differences in repeat periods and copy numbers of repeats in flocculin genes that are likely related to the differential adhesion of Sb as compared to Sc. Core-proteome based taxonomy places Sb strains along with wine strains of Sc. We find the introgression of five genes from Z. bailii into the chromosome IV of Sb and wine strains of Sc. Intriguingly, genes involved in conferring known probiotic properties to Sb are conserved in most Sc strains.
format	article
author	Indu Khatri Rajul Tomar K. Ganesan G. S. Prasad Srikrishna Subramanian
author_facet	Indu Khatri Rajul Tomar K. Ganesan G. S. Prasad Srikrishna Subramanian
author_sort	Indu Khatri
title	Complete genome sequence and comparative genomics of the probiotic yeast Saccharomyces boulardii
title_short	Complete genome sequence and comparative genomics of the probiotic yeast Saccharomyces boulardii
title_full	Complete genome sequence and comparative genomics of the probiotic yeast Saccharomyces boulardii
title_fullStr	Complete genome sequence and comparative genomics of the probiotic yeast Saccharomyces boulardii
title_full_unstemmed	Complete genome sequence and comparative genomics of the probiotic yeast Saccharomyces boulardii
title_sort	complete genome sequence and comparative genomics of the probiotic yeast saccharomyces boulardii
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/9e93e773e2ad4d18ad8274186469255b
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Complete genome sequence and comparative genomics of the probiotic yeast Saccharomyces boulardii

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