MicroRNA induction by copy number gain is associated with poor outcome in squamous cell carcinoma of the lung

Abstract Copy number gains in cancer genomes have been shown to induce oncogene expression and promote carcinogenesis; however, their role in regulating oncogenic microRNAs (onco-miRNAs) remains largely unknown. Our aim was to identify onco-miRNAs induced by copy number gains in human squamous cell...

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Autores principales:	Endi Xia, Sotaro Kanematsu, Yusuke Suenaga, Asmaa Elzawahry, Hitomi Kondo, Noriko Otsuka, Yasumitsu Moriya, Toshihiko Iizasa, Mamoru Kato, Ichiro Yoshino, Sana Yokoi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Copy Number Alternations The Cancer Genome Atlas (TCGA) Induce Oncogene Expression miRNA Mimic Transfection Differentially Expressed miRNA (DEMI) Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9e94e1852eac42cc81b1c053060701a0
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spelling	oai:doaj.org-article:9e94e1852eac42cc81b1c053060701a02021-12-02T15:08:38ZMicroRNA induction by copy number gain is associated with poor outcome in squamous cell carcinoma of the lung10.1038/s41598-018-33696-12045-2322https://doaj.org/article/9e94e1852eac42cc81b1c053060701a02018-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-33696-1https://doaj.org/toc/2045-2322Abstract Copy number gains in cancer genomes have been shown to induce oncogene expression and promote carcinogenesis; however, their role in regulating oncogenic microRNAs (onco-miRNAs) remains largely unknown. Our aim was to identify onco-miRNAs induced by copy number gains in human squamous cell carcinoma (Sq) of the lung. We performed a genome-wide screen of onco-miRNAs from 245 Sqs using data sets from RNA-sequencing, comparative genomic hybridization, and the corresponding clinical information from The Cancer Genome Atlas. Among 1001 miRNAs expressed in the samples, 231 were correlated with copy number alternations, with only 11 of these being highly expressed in Sq compared to adenocarcinoma and normal tissues. Notably, miR-296-5p, miR-324-3p, and miR-3928-3p expression was significantly associated with poor prognosis. Multivariate analysis using the Cox proportional hazards model showed that miRNA expression and smoking were independent prognostic factors and were associated with poor prognosis. Furthermore, the three onco-miRNAs inhibited FAM46C to induce MYC expression, promoting proliferation of Sq cells. We found that copy number gains in Sq of the lung induce onco-miRNA expression that is associated with poor prognosis.Endi XiaSotaro KanematsuYusuke SuenagaAsmaa ElzawahryHitomi KondoNoriko OtsukaYasumitsu MoriyaToshihiko IizasaMamoru KatoIchiro YoshinoSana YokoiNature PortfolioarticleCopy Number AlternationsThe Cancer Genome Atlas (TCGA)Induce Oncogene ExpressionmiRNA Mimic TransfectionDifferentially Expressed miRNA (DEMI)MedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-10 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Copy Number Alternations The Cancer Genome Atlas (TCGA) Induce Oncogene Expression miRNA Mimic Transfection Differentially Expressed miRNA (DEMI) Medicine R Science Q
spellingShingle	Copy Number Alternations The Cancer Genome Atlas (TCGA) Induce Oncogene Expression miRNA Mimic Transfection Differentially Expressed miRNA (DEMI) Medicine R Science Q Endi Xia Sotaro Kanematsu Yusuke Suenaga Asmaa Elzawahry Hitomi Kondo Noriko Otsuka Yasumitsu Moriya Toshihiko Iizasa Mamoru Kato Ichiro Yoshino Sana Yokoi MicroRNA induction by copy number gain is associated with poor outcome in squamous cell carcinoma of the lung
description	Abstract Copy number gains in cancer genomes have been shown to induce oncogene expression and promote carcinogenesis; however, their role in regulating oncogenic microRNAs (onco-miRNAs) remains largely unknown. Our aim was to identify onco-miRNAs induced by copy number gains in human squamous cell carcinoma (Sq) of the lung. We performed a genome-wide screen of onco-miRNAs from 245 Sqs using data sets from RNA-sequencing, comparative genomic hybridization, and the corresponding clinical information from The Cancer Genome Atlas. Among 1001 miRNAs expressed in the samples, 231 were correlated with copy number alternations, with only 11 of these being highly expressed in Sq compared to adenocarcinoma and normal tissues. Notably, miR-296-5p, miR-324-3p, and miR-3928-3p expression was significantly associated with poor prognosis. Multivariate analysis using the Cox proportional hazards model showed that miRNA expression and smoking were independent prognostic factors and were associated with poor prognosis. Furthermore, the three onco-miRNAs inhibited FAM46C to induce MYC expression, promoting proliferation of Sq cells. We found that copy number gains in Sq of the lung induce onco-miRNA expression that is associated with poor prognosis.
format	article
author	Endi Xia Sotaro Kanematsu Yusuke Suenaga Asmaa Elzawahry Hitomi Kondo Noriko Otsuka Yasumitsu Moriya Toshihiko Iizasa Mamoru Kato Ichiro Yoshino Sana Yokoi
author_facet	Endi Xia Sotaro Kanematsu Yusuke Suenaga Asmaa Elzawahry Hitomi Kondo Noriko Otsuka Yasumitsu Moriya Toshihiko Iizasa Mamoru Kato Ichiro Yoshino Sana Yokoi
author_sort	Endi Xia
title	MicroRNA induction by copy number gain is associated with poor outcome in squamous cell carcinoma of the lung
title_short	MicroRNA induction by copy number gain is associated with poor outcome in squamous cell carcinoma of the lung
title_full	MicroRNA induction by copy number gain is associated with poor outcome in squamous cell carcinoma of the lung
title_fullStr	MicroRNA induction by copy number gain is associated with poor outcome in squamous cell carcinoma of the lung
title_full_unstemmed	MicroRNA induction by copy number gain is associated with poor outcome in squamous cell carcinoma of the lung
title_sort	microrna induction by copy number gain is associated with poor outcome in squamous cell carcinoma of the lung
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/9e94e1852eac42cc81b1c053060701a0
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MicroRNA induction by copy number gain is associated with poor outcome in squamous cell carcinoma of the lung

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