Sequential determination of viral load, humoral responses and phylogenetic analysis in fatal and non-fatal cases of Crimean-Congo hemorrhagic fever patients from Gujarat, India, 2019.

<h4>Background</h4>Thirty-four CCHF cases (17 fatal; 17 survived) were confirmed from Gujarat state, India during the year 2019. We aimed to find out the viral load, antibody kinetics, cytokine profile and phylogenetic analysis between fatal and non- fatal cases.<h4>Methods</h4&...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Rima R Sahay, Anita M Shete, Pragya D Yadav, Savita Patil, Triparna Majumdar, Rajlaxmi Jain, Dimpal A Nyayanit, Himanshu Kaushal, Sunil J Panjwani, Kamlesh J Upadhyay, Chetan L Varevadiya, Alpesh Vora, Amit Kanani, Raman R Gangakhedkar
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270
Acceso en línea:	https://doaj.org/article/9ead379ca0924b128ede6e5d090d9485
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:9ead379ca0924b128ede6e5d090d9485
record_format	dspace
spelling	oai:doaj.org-article:9ead379ca0924b128ede6e5d090d94852021-12-02T20:24:14ZSequential determination of viral load, humoral responses and phylogenetic analysis in fatal and non-fatal cases of Crimean-Congo hemorrhagic fever patients from Gujarat, India, 2019.1935-27271935-273510.1371/journal.pntd.0009718https://doaj.org/article/9ead379ca0924b128ede6e5d090d94852021-08-01T00:00:00Zhttps://doi.org/10.1371/journal.pntd.0009718https://doaj.org/toc/1935-2727https://doaj.org/toc/1935-2735<h4>Background</h4>Thirty-four CCHF cases (17 fatal; 17 survived) were confirmed from Gujarat state, India during the year 2019. We aimed to find out the viral load, antibody kinetics, cytokine profile and phylogenetic analysis between fatal and non- fatal cases.<h4>Methods</h4>Thirty four cases were included in this study. Blood and urine samples were collected from all the cases on the day of admission to hospital. Non-fatal cases were followed weekly for understanding the profile of viral kinetics, anti-CCHFV IgM and IgG antibodies. We also quantified the cytokines in both fatal and non-fatal cases. For epidemiological correlation, livestock were screened for anti-CCHF IgG antibodies and the tick pool specimens were tested by real time RT-PCR. Virus isolation was attempted on tick pools and human specimens and phylogenetic analysis performed on human and ticks complete genome sequences.<h4>Results</h4>CCHF cases were detected throughout year in 2019 with the peak in August. Out of 34 cases, eight secondary CCHF cases were reported. Cases were predominantly detected in males and in 19-45 years age group (55.88%). The persistence of viremia was observed till 76th POD (post onset date) in one case whereas anti-CCHFV IgM and IgG was detected amongst these cases from the 2nd and 20th POD respectively. Positivity observed amongst livestock and tick pools were was 21.57% and 7.4% respectively. The cytokine analysis revealed a significant increase in the level of serum IL-6, IL-10 and IFN-γ during the acute phase of the infection, but interestingly IL-10 lowered to normal upon clearance of the virus in the clinically recovered case. Fatal cases had high viral RNA copy numbers. Bleeding from one or two mucosal sites was significantly associated with fatality (OR-16.47;p-0.0034 at 95% CI). We could do CCHF virus isolation from two cases. Phylogenetic analysis revealed circulation of re-assortment of Asian-West African genotypes in humans and ticks.<h4>Conclusions</h4>The persistence of CCHF viral RNA was detected till 76th POD in one of the survivors. The circulation of a re-assortment Asian-West African genotype in a CCHF case is also reported first time from India.Rima R SahayAnita M ShetePragya D YadavSavita PatilTriparna MajumdarRajlaxmi JainDimpal A NyayanitHimanshu KaushalSunil J PanjwaniKamlesh J UpadhyayChetan L VarevadiyaAlpesh VoraAmit KananiRaman R GangakhedkarPublic Library of Science (PLoS)articleArctic medicine. Tropical medicineRC955-962Public aspects of medicineRA1-1270ENPLoS Neglected Tropical Diseases, Vol 15, Iss 8, p e0009718 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270
spellingShingle	Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Rima R Sahay Anita M Shete Pragya D Yadav Savita Patil Triparna Majumdar Rajlaxmi Jain Dimpal A Nyayanit Himanshu Kaushal Sunil J Panjwani Kamlesh J Upadhyay Chetan L Varevadiya Alpesh Vora Amit Kanani Raman R Gangakhedkar Sequential determination of viral load, humoral responses and phylogenetic analysis in fatal and non-fatal cases of Crimean-Congo hemorrhagic fever patients from Gujarat, India, 2019.
description	<h4>Background</h4>Thirty-four CCHF cases (17 fatal; 17 survived) were confirmed from Gujarat state, India during the year 2019. We aimed to find out the viral load, antibody kinetics, cytokine profile and phylogenetic analysis between fatal and non- fatal cases.<h4>Methods</h4>Thirty four cases were included in this study. Blood and urine samples were collected from all the cases on the day of admission to hospital. Non-fatal cases were followed weekly for understanding the profile of viral kinetics, anti-CCHFV IgM and IgG antibodies. We also quantified the cytokines in both fatal and non-fatal cases. For epidemiological correlation, livestock were screened for anti-CCHF IgG antibodies and the tick pool specimens were tested by real time RT-PCR. Virus isolation was attempted on tick pools and human specimens and phylogenetic analysis performed on human and ticks complete genome sequences.<h4>Results</h4>CCHF cases were detected throughout year in 2019 with the peak in August. Out of 34 cases, eight secondary CCHF cases were reported. Cases were predominantly detected in males and in 19-45 years age group (55.88%). The persistence of viremia was observed till 76th POD (post onset date) in one case whereas anti-CCHFV IgM and IgG was detected amongst these cases from the 2nd and 20th POD respectively. Positivity observed amongst livestock and tick pools were was 21.57% and 7.4% respectively. The cytokine analysis revealed a significant increase in the level of serum IL-6, IL-10 and IFN-γ during the acute phase of the infection, but interestingly IL-10 lowered to normal upon clearance of the virus in the clinically recovered case. Fatal cases had high viral RNA copy numbers. Bleeding from one or two mucosal sites was significantly associated with fatality (OR-16.47;p-0.0034 at 95% CI). We could do CCHF virus isolation from two cases. Phylogenetic analysis revealed circulation of re-assortment of Asian-West African genotypes in humans and ticks.<h4>Conclusions</h4>The persistence of CCHF viral RNA was detected till 76th POD in one of the survivors. The circulation of a re-assortment Asian-West African genotype in a CCHF case is also reported first time from India.
format	article
author	Rima R Sahay Anita M Shete Pragya D Yadav Savita Patil Triparna Majumdar Rajlaxmi Jain Dimpal A Nyayanit Himanshu Kaushal Sunil J Panjwani Kamlesh J Upadhyay Chetan L Varevadiya Alpesh Vora Amit Kanani Raman R Gangakhedkar
author_facet	Rima R Sahay Anita M Shete Pragya D Yadav Savita Patil Triparna Majumdar Rajlaxmi Jain Dimpal A Nyayanit Himanshu Kaushal Sunil J Panjwani Kamlesh J Upadhyay Chetan L Varevadiya Alpesh Vora Amit Kanani Raman R Gangakhedkar
author_sort	Rima R Sahay
title	Sequential determination of viral load, humoral responses and phylogenetic analysis in fatal and non-fatal cases of Crimean-Congo hemorrhagic fever patients from Gujarat, India, 2019.
title_short	Sequential determination of viral load, humoral responses and phylogenetic analysis in fatal and non-fatal cases of Crimean-Congo hemorrhagic fever patients from Gujarat, India, 2019.
title_full	Sequential determination of viral load, humoral responses and phylogenetic analysis in fatal and non-fatal cases of Crimean-Congo hemorrhagic fever patients from Gujarat, India, 2019.
title_fullStr	Sequential determination of viral load, humoral responses and phylogenetic analysis in fatal and non-fatal cases of Crimean-Congo hemorrhagic fever patients from Gujarat, India, 2019.
title_full_unstemmed	Sequential determination of viral load, humoral responses and phylogenetic analysis in fatal and non-fatal cases of Crimean-Congo hemorrhagic fever patients from Gujarat, India, 2019.
title_sort	sequential determination of viral load, humoral responses and phylogenetic analysis in fatal and non-fatal cases of crimean-congo hemorrhagic fever patients from gujarat, india, 2019.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/9ead379ca0924b128ede6e5d090d9485
work_keys_str_mv	AT rimarsahay sequentialdeterminationofviralloadhumoralresponsesandphylogeneticanalysisinfatalandnonfatalcasesofcrimeancongohemorrhagicfeverpatientsfromgujaratindia2019 AT anitamshete sequentialdeterminationofviralloadhumoralresponsesandphylogeneticanalysisinfatalandnonfatalcasesofcrimeancongohemorrhagicfeverpatientsfromgujaratindia2019 AT pragyadyadav sequentialdeterminationofviralloadhumoralresponsesandphylogeneticanalysisinfatalandnonfatalcasesofcrimeancongohemorrhagicfeverpatientsfromgujaratindia2019 AT savitapatil sequentialdeterminationofviralloadhumoralresponsesandphylogeneticanalysisinfatalandnonfatalcasesofcrimeancongohemorrhagicfeverpatientsfromgujaratindia2019 AT triparnamajumdar sequentialdeterminationofviralloadhumoralresponsesandphylogeneticanalysisinfatalandnonfatalcasesofcrimeancongohemorrhagicfeverpatientsfromgujaratindia2019 AT rajlaxmijain sequentialdeterminationofviralloadhumoralresponsesandphylogeneticanalysisinfatalandnonfatalcasesofcrimeancongohemorrhagicfeverpatientsfromgujaratindia2019 AT dimpalanyayanit sequentialdeterminationofviralloadhumoralresponsesandphylogeneticanalysisinfatalandnonfatalcasesofcrimeancongohemorrhagicfeverpatientsfromgujaratindia2019 AT himanshukaushal sequentialdeterminationofviralloadhumoralresponsesandphylogeneticanalysisinfatalandnonfatalcasesofcrimeancongohemorrhagicfeverpatientsfromgujaratindia2019 AT suniljpanjwani sequentialdeterminationofviralloadhumoralresponsesandphylogeneticanalysisinfatalandnonfatalcasesofcrimeancongohemorrhagicfeverpatientsfromgujaratindia2019 AT kamleshjupadhyay sequentialdeterminationofviralloadhumoralresponsesandphylogeneticanalysisinfatalandnonfatalcasesofcrimeancongohemorrhagicfeverpatientsfromgujaratindia2019 AT chetanlvarevadiya sequentialdeterminationofviralloadhumoralresponsesandphylogeneticanalysisinfatalandnonfatalcasesofcrimeancongohemorrhagicfeverpatientsfromgujaratindia2019 AT alpeshvora sequentialdeterminationofviralloadhumoralresponsesandphylogeneticanalysisinfatalandnonfatalcasesofcrimeancongohemorrhagicfeverpatientsfromgujaratindia2019 AT amitkanani sequentialdeterminationofviralloadhumoralresponsesandphylogeneticanalysisinfatalandnonfatalcasesofcrimeancongohemorrhagicfeverpatientsfromgujaratindia2019 AT ramanrgangakhedkar sequentialdeterminationofviralloadhumoralresponsesandphylogeneticanalysisinfatalandnonfatalcasesofcrimeancongohemorrhagicfeverpatientsfromgujaratindia2019
_version_	1718374066934513664

Sequential determination of viral load, humoral responses and phylogenetic analysis in fatal and non-fatal cases of Crimean-Congo hemorrhagic fever patients from Gujarat, India, 2019.

Ejemplares similares