Blastocyst complementation using Prdm14-deficient rats enables efficient germline transmission and generation of functional mouse spermatids in rats

Blastocyst complementation using Prdm14-deficient rats enables efficient germline transmission and generation of functional mouse spermatids in rats

The uptake of donor pluripotent stem cells (PSCs) in hosts of different species and subsequent germline transmission is very inefficient. Here, the authors show, using Prdm14 gene depleted rat host blastocysts to remove functional sperm, that germline transmission from donor rat or mouse PSCs is pos...

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Autores principales: Toshihiro Kobayashi, Teppei Goto, Mami Oikawa, Makoto Sanbo, Fumika Yoshida, Reiko Terada, Naoko Niizeki, Naoyo Kajitani, Kanako Kazuki, Yasuhiro Kazuki, Shinichi Hochi, Hiromitsu Nakauchi, M. Azim Surani, Masumi Hirabayashi
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2021
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Acceso en línea:https://doaj.org/article/9eb88f2e20b34fab81b008931d8f11b3
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spelling oai:doaj.org-article:9eb88f2e20b34fab81b008931d8f11b32021-12-02T13:34:29ZBlastocyst complementation using Prdm14-deficient rats enables efficient germline transmission and generation of functional mouse spermatids in rats10.1038/s41467-021-21557-x2041-1723https://doaj.org/article/9eb88f2e20b34fab81b008931d8f11b32021-02-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-21557-xhttps://doaj.org/toc/2041-1723The uptake of donor pluripotent stem cells (PSCs) in hosts of different species and subsequent germline transmission is very inefficient. Here, the authors show, using Prdm14 gene depleted rat host blastocysts to remove functional sperm, that germline transmission from donor rat or mouse PSCs is possible.Toshihiro KobayashiTeppei GotoMami OikawaMakoto SanboFumika YoshidaReiko TeradaNaoko NiizekiNaoyo KajitaniKanako KazukiYasuhiro KazukiShinichi HochiHiromitsu NakauchiM. Azim SuraniMasumi HirabayashiNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-10 (2021)
institution DOAJ
collection DOAJ
language EN
topic Science
Q
spellingShingle Science
Q
Toshihiro Kobayashi
Teppei Goto
Mami Oikawa
Makoto Sanbo
Fumika Yoshida
Reiko Terada
Naoko Niizeki
Naoyo Kajitani
Kanako Kazuki
Yasuhiro Kazuki
Shinichi Hochi
Hiromitsu Nakauchi
M. Azim Surani
Masumi Hirabayashi
Blastocyst complementation using Prdm14-deficient rats enables efficient germline transmission and generation of functional mouse spermatids in rats
description The uptake of donor pluripotent stem cells (PSCs) in hosts of different species and subsequent germline transmission is very inefficient. Here, the authors show, using Prdm14 gene depleted rat host blastocysts to remove functional sperm, that germline transmission from donor rat or mouse PSCs is possible.
format article
author Toshihiro Kobayashi
Teppei Goto
Mami Oikawa
Makoto Sanbo
Fumika Yoshida
Reiko Terada
Naoko Niizeki
Naoyo Kajitani
Kanako Kazuki
Yasuhiro Kazuki
Shinichi Hochi
Hiromitsu Nakauchi
M. Azim Surani
Masumi Hirabayashi
author_facet Toshihiro Kobayashi
Teppei Goto
Mami Oikawa
Makoto Sanbo
Fumika Yoshida
Reiko Terada
Naoko Niizeki
Naoyo Kajitani
Kanako Kazuki
Yasuhiro Kazuki
Shinichi Hochi
Hiromitsu Nakauchi
M. Azim Surani
Masumi Hirabayashi
author_sort Toshihiro Kobayashi
title Blastocyst complementation using Prdm14-deficient rats enables efficient germline transmission and generation of functional mouse spermatids in rats
title_short Blastocyst complementation using Prdm14-deficient rats enables efficient germline transmission and generation of functional mouse spermatids in rats
title_full Blastocyst complementation using Prdm14-deficient rats enables efficient germline transmission and generation of functional mouse spermatids in rats
title_fullStr Blastocyst complementation using Prdm14-deficient rats enables efficient germline transmission and generation of functional mouse spermatids in rats
title_full_unstemmed Blastocyst complementation using Prdm14-deficient rats enables efficient germline transmission and generation of functional mouse spermatids in rats
title_sort blastocyst complementation using prdm14-deficient rats enables efficient germline transmission and generation of functional mouse spermatids in rats
publisher Nature Portfolio
publishDate 2021
url https://doaj.org/article/9eb88f2e20b34fab81b008931d8f11b3
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