TXNDC2 joint molecular marker is associated with testis pathology and is an accurate predictor of sperm retrieval

Abstract The association of PRM1/2 with male azoospermia is well-documented, but the relationship between TXNDC2 deficiency and the azoospermia phenotype, sperm retrieval, and pathology has not been elucidated. Here we identified the association of TXNDC2 and protamines in evaluating testis patholog...

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Autores principales:	Seyed-Morteza Javadirad, Mohammad Mokhtari
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9ebaff5331e040a690e5435d2dea72ab
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spelling	oai:doaj.org-article:9ebaff5331e040a690e5435d2dea72ab2021-12-02T17:12:25ZTXNDC2 joint molecular marker is associated with testis pathology and is an accurate predictor of sperm retrieval10.1038/s41598-021-92603-32045-2322https://doaj.org/article/9ebaff5331e040a690e5435d2dea72ab2021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-92603-3https://doaj.org/toc/2045-2322Abstract The association of PRM1/2 with male azoospermia is well-documented, but the relationship between TXNDC2 deficiency and the azoospermia phenotype, sperm retrieval, and pathology has not been elucidated. Here we identified the association of TXNDC2 and protamines in evaluating testis pathology and sperm retrieval. An extensive microarray meta-analysis of men with idiopathic azoospermia was performed, and after undergoing several steps of data quality controls, the data passing QC were pooled and batch effect corrected. As redox imbalance has been shown to have a variable relationship with fertility, our relative expression studies began with candidate protamination and thioredoxin genes. We constructed a logistic regression model of TXNDC2 with PRM1 and PRM2 genes, and collective ROC analysis indicated a sensitivity of 96.8% and specificity of 95.5% with a ROC value of 0.995 (SE = 0.0070, 95% CI 0.982–1.000). These results demonstrate that TXNDC2, PRM1, and PRM2 combined have a robust power to predict sperm retrieval and correlate with severe azoospermia pathology.Seyed-Morteza JavadiradMohammad MokhtariNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Seyed-Morteza Javadirad Mohammad Mokhtari TXNDC2 joint molecular marker is associated with testis pathology and is an accurate predictor of sperm retrieval
description	Abstract The association of PRM1/2 with male azoospermia is well-documented, but the relationship between TXNDC2 deficiency and the azoospermia phenotype, sperm retrieval, and pathology has not been elucidated. Here we identified the association of TXNDC2 and protamines in evaluating testis pathology and sperm retrieval. An extensive microarray meta-analysis of men with idiopathic azoospermia was performed, and after undergoing several steps of data quality controls, the data passing QC were pooled and batch effect corrected. As redox imbalance has been shown to have a variable relationship with fertility, our relative expression studies began with candidate protamination and thioredoxin genes. We constructed a logistic regression model of TXNDC2 with PRM1 and PRM2 genes, and collective ROC analysis indicated a sensitivity of 96.8% and specificity of 95.5% with a ROC value of 0.995 (SE = 0.0070, 95% CI 0.982–1.000). These results demonstrate that TXNDC2, PRM1, and PRM2 combined have a robust power to predict sperm retrieval and correlate with severe azoospermia pathology.
format	article
author	Seyed-Morteza Javadirad Mohammad Mokhtari
author_facet	Seyed-Morteza Javadirad Mohammad Mokhtari
author_sort	Seyed-Morteza Javadirad
title	TXNDC2 joint molecular marker is associated with testis pathology and is an accurate predictor of sperm retrieval
title_short	TXNDC2 joint molecular marker is associated with testis pathology and is an accurate predictor of sperm retrieval
title_full	TXNDC2 joint molecular marker is associated with testis pathology and is an accurate predictor of sperm retrieval
title_fullStr	TXNDC2 joint molecular marker is associated with testis pathology and is an accurate predictor of sperm retrieval
title_full_unstemmed	TXNDC2 joint molecular marker is associated with testis pathology and is an accurate predictor of sperm retrieval
title_sort	txndc2 joint molecular marker is associated with testis pathology and is an accurate predictor of sperm retrieval
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/9ebaff5331e040a690e5435d2dea72ab
work_keys_str_mv	AT seyedmortezajavadirad txndc2jointmolecularmarkerisassociatedwithtestispathologyandisanaccuratepredictorofspermretrieval AT mohammadmokhtari txndc2jointmolecularmarkerisassociatedwithtestispathologyandisanaccuratepredictorofspermretrieval
_version_	1718381393867702272

TXNDC2 joint molecular marker is associated with testis pathology and is an accurate predictor of sperm retrieval

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