In vitro behavior of tendon stem/progenitor cells on bioactive electrospun nanofiber membranes for tendon-bone tissue engineering applications

Yucheng Lin,1-3,* Lu Zhang,4,* Nancy Q Liu,5 Qingqiang Yao,1,2 Ben Van Handel,5 Yan Xu,1,2 Chen Wang,3 Denis Evseenko,5 Liming Wang1,21Department of Orthopaedic Surgery, Nanjing First Hospital, Nanjing Medical University, Nanjing, Jiangsu, People’s Republic of Ch...

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Autores principales:	Lin Y, Zhang L, Liu NQ, Yao Q, Van Handel B, Xu Y, Wang C, Evseenko D, Wang L
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	Electrospinning TSPCs Nanomaterial Biomimetic scaffold Osteogenic differentiation tendon-bone healing Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/9ebd0e61c3a8493d8c87784e387c7221
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spelling	oai:doaj.org-article:9ebd0e61c3a8493d8c87784e387c72212021-12-02T06:36:31ZIn vitro behavior of tendon stem/progenitor cells on bioactive electrospun nanofiber membranes for tendon-bone tissue engineering applications1178-2013https://doaj.org/article/9ebd0e61c3a8493d8c87784e387c72212019-07-01T00:00:00Zhttps://www.dovepress.com/in-vitro-behavior-of-tendon-stemprogenitor-cells-on-bioactive-electros-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Yucheng Lin,1-3,* Lu Zhang,4,* Nancy Q Liu,5 Qingqiang Yao,1,2 Ben Van Handel,5 Yan Xu,1,2 Chen Wang,3 Denis Evseenko,5 Liming Wang1,21Department of Orthopaedic Surgery, Nanjing First Hospital, Nanjing Medical University, Nanjing, Jiangsu, People’s Republic of China; 2Digital Medicine Institute, Nanjing Medical University, Nanjing, Jiangsu, People’s Republic of China; 3Department of Orthopaedic Surgery, Zhongda Hospital, School of Medicine, Southeast University, Nanjing, Jiangsu, People’s Republic of China; 4Department of Anesthesiology, Women’s Hospital of Nanjing Medical University, Nanjing Maternity and Child Health Care Hospital, Nanjing, Jiangsu, People’s Republic of China; 5Department of Orthopaedic Surgery, University of Southern California (USC), Los Angeles, CA, USA*These authors contributed equally to this workPurpose: In order to accelerate the tendon-bone healing processes and achieve the efficient osteointegration between the tendon graft and bone tunnel, we aim to design bioactive electrospun nanofiber membranes combined with tendon stem/progenitor cells (TSPCs) to promote osteogenic regeneration of the tendon and bone interface.Methods: In this study, nanofiber membranes of polycaprolactone (PCL), PCL/collagen I (COL-1) hybrid nanofiber membranes, poly(dopamine) (PDA)-coated PCL nanofiber membranes and PDA-coated PCL/COL-1 hybrid nanofiber membranes were successfully fabricated by electrospinning. The biochemical characteristics and nanofibrous morphology of the membranes, as well as the characterization of rat TSPCs, were defined in vitro. After co-culture with different types of electrospun nanofiber membranes in vitro, cell proliferation, viability, adhesion and osteogenic differentiation of TSPCs were evaluated at different time points.Results: Among all the membranes, the performance of the PCL/COL-1 (volume ratio: 2:1 v/v) group was superior in terms of its ability to support the adhesion, proliferation, and osteogenic differentiation of TSPCs. No benefit was found in this study to include PDA coating on cell adhesion, proliferation and osteogenic differentiation of TSPCs.Conclusion: The PCL/COL-1 hybrid electrospun nanofiber membranes are biocompatible, biomimetic, easily fabricated, and are capable of supporting cell adhesion, proliferation, and osteogenic differentiation of TSPCs. These bioactive electrospun nanofiber membranes may act as a suitable functional biomimetic scaffold in tendon-bone tissue engineering applications to enhance tendon-bone healing abilities.Keywords: electrospinning, TSPCs, nanomaterial, biomimetic scaffold, osteogenic differentiation, tendon-bone healingLin YZhang LLiu NQYao QVan Handel BXu YWang CEvseenko DWang LDove Medical PressarticleElectrospinningTSPCsNanomaterialBiomimetic scaffoldOsteogenic differentiationtendon-bone healingMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 14, Pp 5831-5848 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Electrospinning TSPCs Nanomaterial Biomimetic scaffold Osteogenic differentiation tendon-bone healing Medicine (General) R5-920
spellingShingle	Electrospinning TSPCs Nanomaterial Biomimetic scaffold Osteogenic differentiation tendon-bone healing Medicine (General) R5-920 Lin Y Zhang L Liu NQ Yao Q Van Handel B Xu Y Wang C Evseenko D Wang L In vitro behavior of tendon stem/progenitor cells on bioactive electrospun nanofiber membranes for tendon-bone tissue engineering applications
description	Yucheng Lin,1-3,* Lu Zhang,4,* Nancy Q Liu,5 Qingqiang Yao,1,2 Ben Van Handel,5 Yan Xu,1,2 Chen Wang,3 Denis Evseenko,5 Liming Wang1,21Department of Orthopaedic Surgery, Nanjing First Hospital, Nanjing Medical University, Nanjing, Jiangsu, People’s Republic of China; 2Digital Medicine Institute, Nanjing Medical University, Nanjing, Jiangsu, People’s Republic of China; 3Department of Orthopaedic Surgery, Zhongda Hospital, School of Medicine, Southeast University, Nanjing, Jiangsu, People’s Republic of China; 4Department of Anesthesiology, Women’s Hospital of Nanjing Medical University, Nanjing Maternity and Child Health Care Hospital, Nanjing, Jiangsu, People’s Republic of China; 5Department of Orthopaedic Surgery, University of Southern California (USC), Los Angeles, CA, USA*These authors contributed equally to this workPurpose: In order to accelerate the tendon-bone healing processes and achieve the efficient osteointegration between the tendon graft and bone tunnel, we aim to design bioactive electrospun nanofiber membranes combined with tendon stem/progenitor cells (TSPCs) to promote osteogenic regeneration of the tendon and bone interface.Methods: In this study, nanofiber membranes of polycaprolactone (PCL), PCL/collagen I (COL-1) hybrid nanofiber membranes, poly(dopamine) (PDA)-coated PCL nanofiber membranes and PDA-coated PCL/COL-1 hybrid nanofiber membranes were successfully fabricated by electrospinning. The biochemical characteristics and nanofibrous morphology of the membranes, as well as the characterization of rat TSPCs, were defined in vitro. After co-culture with different types of electrospun nanofiber membranes in vitro, cell proliferation, viability, adhesion and osteogenic differentiation of TSPCs were evaluated at different time points.Results: Among all the membranes, the performance of the PCL/COL-1 (volume ratio: 2:1 v/v) group was superior in terms of its ability to support the adhesion, proliferation, and osteogenic differentiation of TSPCs. No benefit was found in this study to include PDA coating on cell adhesion, proliferation and osteogenic differentiation of TSPCs.Conclusion: The PCL/COL-1 hybrid electrospun nanofiber membranes are biocompatible, biomimetic, easily fabricated, and are capable of supporting cell adhesion, proliferation, and osteogenic differentiation of TSPCs. These bioactive electrospun nanofiber membranes may act as a suitable functional biomimetic scaffold in tendon-bone tissue engineering applications to enhance tendon-bone healing abilities.Keywords: electrospinning, TSPCs, nanomaterial, biomimetic scaffold, osteogenic differentiation, tendon-bone healing
format	article
author	Lin Y Zhang L Liu NQ Yao Q Van Handel B Xu Y Wang C Evseenko D Wang L
author_facet	Lin Y Zhang L Liu NQ Yao Q Van Handel B Xu Y Wang C Evseenko D Wang L
author_sort	Lin Y
title	In vitro behavior of tendon stem/progenitor cells on bioactive electrospun nanofiber membranes for tendon-bone tissue engineering applications
title_short	In vitro behavior of tendon stem/progenitor cells on bioactive electrospun nanofiber membranes for tendon-bone tissue engineering applications
title_full	In vitro behavior of tendon stem/progenitor cells on bioactive electrospun nanofiber membranes for tendon-bone tissue engineering applications
title_fullStr	In vitro behavior of tendon stem/progenitor cells on bioactive electrospun nanofiber membranes for tendon-bone tissue engineering applications
title_full_unstemmed	In vitro behavior of tendon stem/progenitor cells on bioactive electrospun nanofiber membranes for tendon-bone tissue engineering applications
title_sort	in vitro behavior of tendon stem/progenitor cells on bioactive electrospun nanofiber membranes for tendon-bone tissue engineering applications
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/9ebd0e61c3a8493d8c87784e387c7221
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In vitro behavior of tendon stem/progenitor cells on bioactive electrospun nanofiber membranes for tendon-bone tissue engineering applications

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