Bacterial diversity among four healthcare-associated institutes in Taiwan

Abstract Indoor microbial communities have important implications for human health, especially in health-care institutes (HCIs). The factors that determine the diversity and composition of microbiomes in a built environment remain unclear. Herein, we used 16S rRNA amplicon sequencing to investigate...

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Autores principales: Chang-Hua Chen, Yaw-Ling Lin, Kuan-Hsueh Chen, Wen-Pei Chen, Zhao-Feng Chen, Han-Yueh Kuo, Hsueh-Fen Hung, Chuan Yi Tang, Ming-Li Liou
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Lenguaje:EN
Publicado: Nature Portfolio 2017
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Acceso en línea:https://doaj.org/article/9ec11ddc57b54d8abf0871842debc91e
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spelling oai:doaj.org-article:9ec11ddc57b54d8abf0871842debc91e2021-12-02T11:40:32ZBacterial diversity among four healthcare-associated institutes in Taiwan10.1038/s41598-017-08679-32045-2322https://doaj.org/article/9ec11ddc57b54d8abf0871842debc91e2017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08679-3https://doaj.org/toc/2045-2322Abstract Indoor microbial communities have important implications for human health, especially in health-care institutes (HCIs). The factors that determine the diversity and composition of microbiomes in a built environment remain unclear. Herein, we used 16S rRNA amplicon sequencing to investigate the relationships between building attributes and surface bacterial communities among four HCIs located in three buildings. We examined the surface bacterial communities and environmental parameters in the buildings supplied with different ventilation types and compared the results using a Dirichlet multinomial mixture (DMM)-based approach. A total of 203 samples from the four HCIs were analyzed. Four bacterial communities were grouped using the DMM-based approach, which were highly similar to those in the 4 HCIs. The α-diversity and β-diversity in the naturally ventilated building were different from the conditioner-ventilated building. The bacterial source composition varied across each building. Nine genera were found as the core microbiota shared by all the areas, of which Acinetobacter, Enterobacter, Pseudomonas, and Staphylococcus are regarded as healthcare-associated pathogens (HAPs). The observed relationship between environmental parameters such as core microbiota and surface bacterial diversity suggests that we might manage indoor environments by creating new sanitation protocols, adjusting the ventilation design, and further understanding the transmission routes of HAPs.Chang-Hua ChenYaw-Ling LinKuan-Hsueh ChenWen-Pei ChenZhao-Feng ChenHan-Yueh KuoHsueh-Fen HungChuan Yi TangMing-Li LiouNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Chang-Hua Chen
Yaw-Ling Lin
Kuan-Hsueh Chen
Wen-Pei Chen
Zhao-Feng Chen
Han-Yueh Kuo
Hsueh-Fen Hung
Chuan Yi Tang
Ming-Li Liou
Bacterial diversity among four healthcare-associated institutes in Taiwan
description Abstract Indoor microbial communities have important implications for human health, especially in health-care institutes (HCIs). The factors that determine the diversity and composition of microbiomes in a built environment remain unclear. Herein, we used 16S rRNA amplicon sequencing to investigate the relationships between building attributes and surface bacterial communities among four HCIs located in three buildings. We examined the surface bacterial communities and environmental parameters in the buildings supplied with different ventilation types and compared the results using a Dirichlet multinomial mixture (DMM)-based approach. A total of 203 samples from the four HCIs were analyzed. Four bacterial communities were grouped using the DMM-based approach, which were highly similar to those in the 4 HCIs. The α-diversity and β-diversity in the naturally ventilated building were different from the conditioner-ventilated building. The bacterial source composition varied across each building. Nine genera were found as the core microbiota shared by all the areas, of which Acinetobacter, Enterobacter, Pseudomonas, and Staphylococcus are regarded as healthcare-associated pathogens (HAPs). The observed relationship between environmental parameters such as core microbiota and surface bacterial diversity suggests that we might manage indoor environments by creating new sanitation protocols, adjusting the ventilation design, and further understanding the transmission routes of HAPs.
format article
author Chang-Hua Chen
Yaw-Ling Lin
Kuan-Hsueh Chen
Wen-Pei Chen
Zhao-Feng Chen
Han-Yueh Kuo
Hsueh-Fen Hung
Chuan Yi Tang
Ming-Li Liou
author_facet Chang-Hua Chen
Yaw-Ling Lin
Kuan-Hsueh Chen
Wen-Pei Chen
Zhao-Feng Chen
Han-Yueh Kuo
Hsueh-Fen Hung
Chuan Yi Tang
Ming-Li Liou
author_sort Chang-Hua Chen
title Bacterial diversity among four healthcare-associated institutes in Taiwan
title_short Bacterial diversity among four healthcare-associated institutes in Taiwan
title_full Bacterial diversity among four healthcare-associated institutes in Taiwan
title_fullStr Bacterial diversity among four healthcare-associated institutes in Taiwan
title_full_unstemmed Bacterial diversity among four healthcare-associated institutes in Taiwan
title_sort bacterial diversity among four healthcare-associated institutes in taiwan
publisher Nature Portfolio
publishDate 2017
url https://doaj.org/article/9ec11ddc57b54d8abf0871842debc91e
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