Combining Materials Obtained by 3D-Printing and Electrospinning from Commercial Polylactide Filament to Produce Biocompatible Composites

The design of scaffolds to reach similar three-dimensional structures mimicking the natural and fibrous environment of some cells is a challenge for tissue engineering, and 3D-printing and electrospinning highlights from other techniques in the production of scaffolds. The former is a well-known add...

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Autores principales:	Pablo Romero-Araya, Victor Pino, Ariel Nenen, Verena Cárdenas, Francisca Pavicic, Pamela Ehrenfeld, Guillaume Serandour, Judit G. Lisoni, Ignacio Moreno-Villoslada, Mario E. Flores
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	biocompatible composites biomimetic composites PLA 3D-printing PLA electrospun fibers Organic chemistry QD241-441
Acceso en línea:	https://doaj.org/article/9ec9b408bffc4496acdb0716c2291af5
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spelling	oai:doaj.org-article:9ec9b408bffc4496acdb0716c2291af52021-11-11T18:47:48ZCombining Materials Obtained by 3D-Printing and Electrospinning from Commercial Polylactide Filament to Produce Biocompatible Composites10.3390/polym132138062073-4360https://doaj.org/article/9ec9b408bffc4496acdb0716c2291af52021-11-01T00:00:00Zhttps://www.mdpi.com/2073-4360/13/21/3806https://doaj.org/toc/2073-4360The design of scaffolds to reach similar three-dimensional structures mimicking the natural and fibrous environment of some cells is a challenge for tissue engineering, and 3D-printing and electrospinning highlights from other techniques in the production of scaffolds. The former is a well-known additive manufacturing technique devoted to the production of custom-made structures with mechanical properties similar to tissues and bones found in the human body, but lacks the resolution to produce small and interconnected structures. The latter is a well-studied technique to produce materials possessing a fibrillar structure, having the advantage of producing materials with tuned composition compared with a 3D-print. Taking the advantage that commercial 3D-printers work with polylactide (PLA) based filaments, a biocompatible and biodegradable polymer, in this work we produce PLA-based composites by blending materials obtained by 3D-printing and electrospinning. Porous PLA fibers have been obtained by the electrospinning of recovered PLA from 3D-printer filaments, tuning the mechanical properties by blending PLA with small amounts of polyethylene glycol and hydroxyapatite. A composite has been obtained by blending two layers of 3D-printed pieces with a central mat of PLA fibers. The composite presented a reduced storage modulus as compared with a single 3D-print piece and possessing similar mechanical properties to bone tissues. Furthermore, the biocompatibility of the composites is assessed by a simulated body fluid assay and by culturing composites with 3T3 fibroblasts. We observed that all these composites induce the growing and attaching of fibroblast over the surface of a 3D-printed layer and in the fibrous layer, showing the potential of commercial 3D-printers and filaments to produce scaffolds to be used in bone tissue engineering.Pablo Romero-ArayaVictor PinoAriel NenenVerena CárdenasFrancisca PavicicPamela EhrenfeldGuillaume SerandourJudit G. LisoniIgnacio Moreno-VillosladaMario E. FloresMDPI AGarticlebiocompatible compositesbiomimetic compositesPLA 3D-printingPLA electrospun fibersOrganic chemistryQD241-441ENPolymers, Vol 13, Iss 3806, p 3806 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	biocompatible composites biomimetic composites PLA 3D-printing PLA electrospun fibers Organic chemistry QD241-441
spellingShingle	biocompatible composites biomimetic composites PLA 3D-printing PLA electrospun fibers Organic chemistry QD241-441 Pablo Romero-Araya Victor Pino Ariel Nenen Verena Cárdenas Francisca Pavicic Pamela Ehrenfeld Guillaume Serandour Judit G. Lisoni Ignacio Moreno-Villoslada Mario E. Flores Combining Materials Obtained by 3D-Printing and Electrospinning from Commercial Polylactide Filament to Produce Biocompatible Composites
description	The design of scaffolds to reach similar three-dimensional structures mimicking the natural and fibrous environment of some cells is a challenge for tissue engineering, and 3D-printing and electrospinning highlights from other techniques in the production of scaffolds. The former is a well-known additive manufacturing technique devoted to the production of custom-made structures with mechanical properties similar to tissues and bones found in the human body, but lacks the resolution to produce small and interconnected structures. The latter is a well-studied technique to produce materials possessing a fibrillar structure, having the advantage of producing materials with tuned composition compared with a 3D-print. Taking the advantage that commercial 3D-printers work with polylactide (PLA) based filaments, a biocompatible and biodegradable polymer, in this work we produce PLA-based composites by blending materials obtained by 3D-printing and electrospinning. Porous PLA fibers have been obtained by the electrospinning of recovered PLA from 3D-printer filaments, tuning the mechanical properties by blending PLA with small amounts of polyethylene glycol and hydroxyapatite. A composite has been obtained by blending two layers of 3D-printed pieces with a central mat of PLA fibers. The composite presented a reduced storage modulus as compared with a single 3D-print piece and possessing similar mechanical properties to bone tissues. Furthermore, the biocompatibility of the composites is assessed by a simulated body fluid assay and by culturing composites with 3T3 fibroblasts. We observed that all these composites induce the growing and attaching of fibroblast over the surface of a 3D-printed layer and in the fibrous layer, showing the potential of commercial 3D-printers and filaments to produce scaffolds to be used in bone tissue engineering.
format	article
author	Pablo Romero-Araya Victor Pino Ariel Nenen Verena Cárdenas Francisca Pavicic Pamela Ehrenfeld Guillaume Serandour Judit G. Lisoni Ignacio Moreno-Villoslada Mario E. Flores
author_facet	Pablo Romero-Araya Victor Pino Ariel Nenen Verena Cárdenas Francisca Pavicic Pamela Ehrenfeld Guillaume Serandour Judit G. Lisoni Ignacio Moreno-Villoslada Mario E. Flores
author_sort	Pablo Romero-Araya
title	Combining Materials Obtained by 3D-Printing and Electrospinning from Commercial Polylactide Filament to Produce Biocompatible Composites
title_short	Combining Materials Obtained by 3D-Printing and Electrospinning from Commercial Polylactide Filament to Produce Biocompatible Composites
title_full	Combining Materials Obtained by 3D-Printing and Electrospinning from Commercial Polylactide Filament to Produce Biocompatible Composites
title_fullStr	Combining Materials Obtained by 3D-Printing and Electrospinning from Commercial Polylactide Filament to Produce Biocompatible Composites
title_full_unstemmed	Combining Materials Obtained by 3D-Printing and Electrospinning from Commercial Polylactide Filament to Produce Biocompatible Composites
title_sort	combining materials obtained by 3d-printing and electrospinning from commercial polylactide filament to produce biocompatible composites
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/9ec9b408bffc4496acdb0716c2291af5
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Combining Materials Obtained by 3D-Printing and Electrospinning from Commercial Polylactide Filament to Produce Biocompatible Composites

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