Physicochemical features and transfection properties of chitosan/poloxamer 188/poly(D,L-lactide-co-glycolide) nanoplexes

Donato Cosco,1,5,* Cinzia Federico,1,2,* Jessica Maiuolo,1 Stefania Bulotta,1 Roberto Molinaro,1,3 Donatella Paolino,1,5 Pierfrancesco Tassone,2,4 Massimo Fresta1Department of Health Sciences, 2Department of Experimental and Clinical Medicine, University Magna Græcia of Catanzaro, Catanza...

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Autores principales:	Cosco D, Federico C, Maiuolo J, Bulotta S, Molinaro R, Paolino D, Tassone P, Fresta M
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2014
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/9ecd3852ad664eb7af39678a53cb7d7e
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spelling	oai:doaj.org-article:9ecd3852ad664eb7af39678a53cb7d7e2021-12-02T06:32:32ZPhysicochemical features and transfection properties of chitosan/poloxamer 188/poly(D,L-lactide-co-glycolide) nanoplexes1178-2013https://doaj.org/article/9ecd3852ad664eb7af39678a53cb7d7e2014-05-01T00:00:00Zhttp://www.dovepress.com/physicochemical-features-and-transfection-properties-of-chitosanpoloxa-a16857https://doaj.org/toc/1178-2013 Donato Cosco,1,5,* Cinzia Federico,1,2,* Jessica Maiuolo,1 Stefania Bulotta,1 Roberto Molinaro,1,3 Donatella Paolino,1,5 Pierfrancesco Tassone,2,4 Massimo Fresta1Department of Health Sciences, 2Department of Experimental and Clinical Medicine, University Magna Græcia of Catanzaro, Catanzaro, Italy; 3Department of NanoMedicine, The Methodist Research Institute, Houston, TX, USA; 4Medical Oncology, Tommaso Campanella Cancer Center, Viale S Venuta, Germaneto, 5Interregional Research Center for Food Safety and Health, University of Catanzaro “Magna Græcia”, Catanzaro, Italy*These authors contributed equally to this paperAbstract: The aim of this study was the evaluation of the effects of two emulsifiers on the physicochemical and technological properties of low molecular weight chitosan/poly (D,L-lactide-co-glycolide) (PLGA) nanoplexes and their transfection efficiency. Nanospheres were prepared using the nanoprecipitation method of the preformed polymer. The mean diameter and surface charge of the nanospheres were investigated by photocorrelation spectroscopy. The degree of binding of the plasmid with the nanoplexes was qualitatively and quantitatively determined. MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) testing was performed using HeLa, RPMI8226, and SKMM1 cell lines. Flow cytometry and confocal laser scanning microscopy were used to determine the degree of cellular transfection and internalization of the nanoplexes into cells, respectively. The nanoplexes had a positive zeta potential, and low amounts of PLGA and poloxamer 188 showed a mean colloidal size of ~200 nm with a polydispersity index of ~0.14. The nanoplexes had suitable entrapment efficiency (80%). In vitro experiments showed that the colloidal nanodevices did not induce significant cytotoxicity. The nanoplexes investigated in this study could represent efficient and useful nonviral devices for gene delivery. Use of low amounts of PLGA and poloxamer 188 enabled development of a nanosphere able to transfect cells efficiently. These nanosystems are a helpful platform for delivery of genetic material while preserving therapeutic efficacy.Keywords: nanoplexes, gene delivery, chitosan, poly(D,L-lactide-co-glycolide), poloxamer 188Cosco DFederico CMaiuolo JBulotta SMolinaro RPaolino DTassone PFresta MDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2014, Iss Issue 1, Pp 2359-2372 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Cosco D Federico C Maiuolo J Bulotta S Molinaro R Paolino D Tassone P Fresta M Physicochemical features and transfection properties of chitosan/poloxamer 188/poly(D,L-lactide-co-glycolide) nanoplexes
description	Donato Cosco,1,5,* Cinzia Federico,1,2,* Jessica Maiuolo,1 Stefania Bulotta,1 Roberto Molinaro,1,3 Donatella Paolino,1,5 Pierfrancesco Tassone,2,4 Massimo Fresta1Department of Health Sciences, 2Department of Experimental and Clinical Medicine, University Magna Græcia of Catanzaro, Catanzaro, Italy; 3Department of NanoMedicine, The Methodist Research Institute, Houston, TX, USA; 4Medical Oncology, Tommaso Campanella Cancer Center, Viale S Venuta, Germaneto, 5Interregional Research Center for Food Safety and Health, University of Catanzaro “Magna Græcia”, Catanzaro, Italy*These authors contributed equally to this paperAbstract: The aim of this study was the evaluation of the effects of two emulsifiers on the physicochemical and technological properties of low molecular weight chitosan/poly (D,L-lactide-co-glycolide) (PLGA) nanoplexes and their transfection efficiency. Nanospheres were prepared using the nanoprecipitation method of the preformed polymer. The mean diameter and surface charge of the nanospheres were investigated by photocorrelation spectroscopy. The degree of binding of the plasmid with the nanoplexes was qualitatively and quantitatively determined. MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) testing was performed using HeLa, RPMI8226, and SKMM1 cell lines. Flow cytometry and confocal laser scanning microscopy were used to determine the degree of cellular transfection and internalization of the nanoplexes into cells, respectively. The nanoplexes had a positive zeta potential, and low amounts of PLGA and poloxamer 188 showed a mean colloidal size of ~200 nm with a polydispersity index of ~0.14. The nanoplexes had suitable entrapment efficiency (80%). In vitro experiments showed that the colloidal nanodevices did not induce significant cytotoxicity. The nanoplexes investigated in this study could represent efficient and useful nonviral devices for gene delivery. Use of low amounts of PLGA and poloxamer 188 enabled development of a nanosphere able to transfect cells efficiently. These nanosystems are a helpful platform for delivery of genetic material while preserving therapeutic efficacy.Keywords: nanoplexes, gene delivery, chitosan, poly(D,L-lactide-co-glycolide), poloxamer 188
format	article
author	Cosco D Federico C Maiuolo J Bulotta S Molinaro R Paolino D Tassone P Fresta M
author_facet	Cosco D Federico C Maiuolo J Bulotta S Molinaro R Paolino D Tassone P Fresta M
author_sort	Cosco D
title	Physicochemical features and transfection properties of chitosan/poloxamer 188/poly(D,L-lactide-co-glycolide) nanoplexes
title_short	Physicochemical features and transfection properties of chitosan/poloxamer 188/poly(D,L-lactide-co-glycolide) nanoplexes
title_full	Physicochemical features and transfection properties of chitosan/poloxamer 188/poly(D,L-lactide-co-glycolide) nanoplexes
title_fullStr	Physicochemical features and transfection properties of chitosan/poloxamer 188/poly(D,L-lactide-co-glycolide) nanoplexes
title_full_unstemmed	Physicochemical features and transfection properties of chitosan/poloxamer 188/poly(D,L-lactide-co-glycolide) nanoplexes
title_sort	physicochemical features and transfection properties of chitosan/poloxamer 188/poly(d,l-lactide-co-glycolide) nanoplexes
publisher	Dove Medical Press
publishDate	2014
url	https://doaj.org/article/9ecd3852ad664eb7af39678a53cb7d7e
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Physicochemical features and transfection properties of chitosan/poloxamer 188/poly(D,L-lactide-co-glycolide) nanoplexes

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