Image-guided transplantation of single cells in the bone marrow of live animals

Abstract Transplantation of a single hematopoietic stem cell is an important method for its functional characterization, but the standard transplantation protocol relies on cell homing to the bone marrow after intravenous injection. Here, we present a method to transplant single cells directly into...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Raphaël Turcotte, Clemens Alt, Judith M. Runnels, Kyoko Ito, Juwell W. Wu, Walid Zaher, Luke J. Mortensen, Lev Silberstein, Daniel C. Côté, Andrew L. Kung, Keisuke Ito, Charles P. Lin
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9ecda607d1944f4292e9b9f4fd7e0a35
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:9ecda607d1944f4292e9b9f4fd7e0a35
record_format	dspace
spelling	oai:doaj.org-article:9ecda607d1944f4292e9b9f4fd7e0a352021-12-02T12:32:19ZImage-guided transplantation of single cells in the bone marrow of live animals10.1038/s41598-017-02896-62045-2322https://doaj.org/article/9ecda607d1944f4292e9b9f4fd7e0a352017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02896-6https://doaj.org/toc/2045-2322Abstract Transplantation of a single hematopoietic stem cell is an important method for its functional characterization, but the standard transplantation protocol relies on cell homing to the bone marrow after intravenous injection. Here, we present a method to transplant single cells directly into the bone marrow of live mice. We developed an optical platform that integrates a multiphoton microscope with a laser ablation unit for microsurgery and an optical tweezer for cell micromanipulation. These tools allow image-guided single cell transplantation with high spatial control. The platform was used to deliver single hematopoietic stem cells. The engraftment of transplants was tracked over time, illustrating that the technique can be useful for studying both normal and malignant stem cells in vivo.Raphaël TurcotteClemens AltJudith M. RunnelsKyoko ItoJuwell W. WuWalid ZaherLuke J. MortensenLev SilbersteinDaniel C. CôtéAndrew L. KungKeisuke ItoCharles P. LinNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Raphaël Turcotte Clemens Alt Judith M. Runnels Kyoko Ito Juwell W. Wu Walid Zaher Luke J. Mortensen Lev Silberstein Daniel C. Côté Andrew L. Kung Keisuke Ito Charles P. Lin Image-guided transplantation of single cells in the bone marrow of live animals
description	Abstract Transplantation of a single hematopoietic stem cell is an important method for its functional characterization, but the standard transplantation protocol relies on cell homing to the bone marrow after intravenous injection. Here, we present a method to transplant single cells directly into the bone marrow of live mice. We developed an optical platform that integrates a multiphoton microscope with a laser ablation unit for microsurgery and an optical tweezer for cell micromanipulation. These tools allow image-guided single cell transplantation with high spatial control. The platform was used to deliver single hematopoietic stem cells. The engraftment of transplants was tracked over time, illustrating that the technique can be useful for studying both normal and malignant stem cells in vivo.
format	article
author	Raphaël Turcotte Clemens Alt Judith M. Runnels Kyoko Ito Juwell W. Wu Walid Zaher Luke J. Mortensen Lev Silberstein Daniel C. Côté Andrew L. Kung Keisuke Ito Charles P. Lin
author_facet	Raphaël Turcotte Clemens Alt Judith M. Runnels Kyoko Ito Juwell W. Wu Walid Zaher Luke J. Mortensen Lev Silberstein Daniel C. Côté Andrew L. Kung Keisuke Ito Charles P. Lin
author_sort	Raphaël Turcotte
title	Image-guided transplantation of single cells in the bone marrow of live animals
title_short	Image-guided transplantation of single cells in the bone marrow of live animals
title_full	Image-guided transplantation of single cells in the bone marrow of live animals
title_fullStr	Image-guided transplantation of single cells in the bone marrow of live animals
title_full_unstemmed	Image-guided transplantation of single cells in the bone marrow of live animals
title_sort	image-guided transplantation of single cells in the bone marrow of live animals
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/9ecda607d1944f4292e9b9f4fd7e0a35
work_keys_str_mv	AT raphaelturcotte imageguidedtransplantationofsinglecellsinthebonemarrowofliveanimals AT clemensalt imageguidedtransplantationofsinglecellsinthebonemarrowofliveanimals AT judithmrunnels imageguidedtransplantationofsinglecellsinthebonemarrowofliveanimals AT kyokoito imageguidedtransplantationofsinglecellsinthebonemarrowofliveanimals AT juwellwwu imageguidedtransplantationofsinglecellsinthebonemarrowofliveanimals AT walidzaher imageguidedtransplantationofsinglecellsinthebonemarrowofliveanimals AT lukejmortensen imageguidedtransplantationofsinglecellsinthebonemarrowofliveanimals AT levsilberstein imageguidedtransplantationofsinglecellsinthebonemarrowofliveanimals AT danielccote imageguidedtransplantationofsinglecellsinthebonemarrowofliveanimals AT andrewlkung imageguidedtransplantationofsinglecellsinthebonemarrowofliveanimals AT keisukeito imageguidedtransplantationofsinglecellsinthebonemarrowofliveanimals AT charlesplin imageguidedtransplantationofsinglecellsinthebonemarrowofliveanimals
_version_	1718394109945708544

Image-guided transplantation of single cells in the bone marrow of live animals

Ejemplares similares