Antimicrobial characterization of silver nanoparticle-coated surfaces by “touch test” method

Antimicrobial characterization of silver nanoparticle-coated surfaces by “touch test” method

Marianne Gunell,1,2 Janne Haapanen,3 Kofi J Brobbey,4 Jarkko J Saarinen,4 Martti Toivakka,4 Jyrki M Mäkelä,3 Pentti Huovinen,1 Erkki Eerola1,2 1Department of Medical Microbiology and Immunology, University of Turku, 2Department of Clinical Microbiology and Immunology, Microbiology...

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Autores principales: Gunell M, Haapanen J, Brobbey KJ, Saarinen JJ, Toivakka M, Mäkelä JM, Huovinen P, Eerola E
Formato: article
Lenguaje:EN
Publicado: Dove Medical Press 2017
Materias:
Silver
nanoparticle
E. coli
S. aureus
LFS
HAI
Medical technology
R855-855.5
Chemical technology
TP1-1185
Acceso en línea:https://doaj.org/article/9ed3cc525af84e70b9bd2977dd3f3639
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spelling oai:doaj.org-article:9ed3cc525af84e70b9bd2977dd3f36392021-12-02T03:43:14ZAntimicrobial characterization of silver nanoparticle-coated surfaces by “touch test” method1177-8903https://doaj.org/article/9ed3cc525af84e70b9bd2977dd3f36392017-11-01T00:00:00Zhttps://www.dovepress.com/antimicrobial-characterization-of-silver-nanoparticle-coated-surfaces--peer-reviewed-article-NSAhttps://doaj.org/toc/1177-8903Marianne Gunell,1,2 Janne Haapanen,3 Kofi J Brobbey,4 Jarkko J Saarinen,4 Martti Toivakka,4 Jyrki M Mäkelä,3 Pentti Huovinen,1 Erkki Eerola1,2 1Department of Medical Microbiology and Immunology, University of Turku, 2Department of Clinical Microbiology and Immunology, Microbiology and Genetics Service Area, Turku University Hospital, Turku, 3Aerosol Physics Laboratory, Department of Physics, Tampere University of Technology, Tampere, 4Laboratory of Paper Coating and Converting, Center for Functional Materials, Åbo Akademi University, Turku, Finland Abstract: Bacterial infections, especially by antimicrobial resistant (AMR) bacteria, are an increasing problem worldwide. AMR is especially a problem with health care-associated infections due to bacteria in hospital environments being easily transferred from patient to patient and from patient to environment, and thus, solutions to prevent bacterial transmission are needed. Hand washing is an effective tool for preventing bacterial infections, but other approaches such as nanoparticle-coated surfaces are also needed. In the current study, direct and indirect liquid flame spray (LFS) method was used to produce silver nanoparticle-coated surfaces. The antimicrobial properties of these nanoparticle surfaces were evaluated with the “touch test” method against Escherichia coli and Staphylococcus aureus. It was shown in this study that in glass samples one silver nanoparticle-coating cycle can inhibit E. coli growth, whereas at least two coating cycles were needed to inhibit S. aureus growth. Silver nanoparticle-coated polyethylene (PE) and PE terephthalate samples did not inhibit bacterial growth as effectively as glass samples: three nanoparticle-coating cycles were needed to inhibit E. coli growth, and more than 30 coating cycles were needed until S. aureus growth was inhibited. To conclude, with the LFS method, it is possible to produce nanostructured large-area antibacterial surfaces which show antibacterial effect against clinically relevant pathogens. Results indicate that the use of silver nanoparticle surfaces in hospital environments could prevent health care-associated infections in vivo. Keywords: silver, nanoparticle, E. coli, S. aureus, LFS, HAI Gunell MHaapanen JBrobbey KJSaarinen JJToivakka MMäkelä JMHuovinen PEerola EDove Medical PressarticleSilvernanoparticleE. coliS. aureusLFSHAIMedical technologyR855-855.5Chemical technologyTP1-1185ENNanotechnology, Science and Applications, Vol Volume 10, Pp 137-145 (2017)
institution DOAJ
collection DOAJ
language EN
topic Silver
nanoparticle
E. coli
S. aureus
LFS
HAI
Medical technology
R855-855.5
Chemical technology
TP1-1185
spellingShingle Silver
nanoparticle
E. coli
S. aureus
LFS
HAI
Medical technology
R855-855.5
Chemical technology
TP1-1185
Gunell M
Haapanen J
Brobbey KJ
Saarinen JJ
Toivakka M
Mäkelä JM
Huovinen P
Eerola E
Antimicrobial characterization of silver nanoparticle-coated surfaces by “touch test” method
description Marianne Gunell,1,2 Janne Haapanen,3 Kofi J Brobbey,4 Jarkko J Saarinen,4 Martti Toivakka,4 Jyrki M Mäkelä,3 Pentti Huovinen,1 Erkki Eerola1,2 1Department of Medical Microbiology and Immunology, University of Turku, 2Department of Clinical Microbiology and Immunology, Microbiology and Genetics Service Area, Turku University Hospital, Turku, 3Aerosol Physics Laboratory, Department of Physics, Tampere University of Technology, Tampere, 4Laboratory of Paper Coating and Converting, Center for Functional Materials, Åbo Akademi University, Turku, Finland Abstract: Bacterial infections, especially by antimicrobial resistant (AMR) bacteria, are an increasing problem worldwide. AMR is especially a problem with health care-associated infections due to bacteria in hospital environments being easily transferred from patient to patient and from patient to environment, and thus, solutions to prevent bacterial transmission are needed. Hand washing is an effective tool for preventing bacterial infections, but other approaches such as nanoparticle-coated surfaces are also needed. In the current study, direct and indirect liquid flame spray (LFS) method was used to produce silver nanoparticle-coated surfaces. The antimicrobial properties of these nanoparticle surfaces were evaluated with the “touch test” method against Escherichia coli and Staphylococcus aureus. It was shown in this study that in glass samples one silver nanoparticle-coating cycle can inhibit E. coli growth, whereas at least two coating cycles were needed to inhibit S. aureus growth. Silver nanoparticle-coated polyethylene (PE) and PE terephthalate samples did not inhibit bacterial growth as effectively as glass samples: three nanoparticle-coating cycles were needed to inhibit E. coli growth, and more than 30 coating cycles were needed until S. aureus growth was inhibited. To conclude, with the LFS method, it is possible to produce nanostructured large-area antibacterial surfaces which show antibacterial effect against clinically relevant pathogens. Results indicate that the use of silver nanoparticle surfaces in hospital environments could prevent health care-associated infections in vivo. Keywords: silver, nanoparticle, E. coli, S. aureus, LFS, HAI 
format article
author Gunell M
Haapanen J
Brobbey KJ
Saarinen JJ
Toivakka M
Mäkelä JM
Huovinen P
Eerola E
author_facet Gunell M
Haapanen J
Brobbey KJ
Saarinen JJ
Toivakka M
Mäkelä JM
Huovinen P
Eerola E
author_sort Gunell M
title Antimicrobial characterization of silver nanoparticle-coated surfaces by “touch test” method
title_short Antimicrobial characterization of silver nanoparticle-coated surfaces by “touch test” method
title_full Antimicrobial characterization of silver nanoparticle-coated surfaces by “touch test” method
title_fullStr Antimicrobial characterization of silver nanoparticle-coated surfaces by “touch test” method
title_full_unstemmed Antimicrobial characterization of silver nanoparticle-coated surfaces by “touch test” method
title_sort antimicrobial characterization of silver nanoparticle-coated surfaces by “touch test” method
publisher Dove Medical Press
publishDate 2017
url https://doaj.org/article/9ed3cc525af84e70b9bd2977dd3f3639
work_keys_str_mv AT gunellm antimicrobialcharacterizationofsilvernanoparticlecoatedsurfacesbyldquotouchtestrdquomethod
AT haapanenj antimicrobialcharacterizationofsilvernanoparticlecoatedsurfacesbyldquotouchtestrdquomethod
AT brobbeykj antimicrobialcharacterizationofsilvernanoparticlecoatedsurfacesbyldquotouchtestrdquomethod
AT saarinenjj antimicrobialcharacterizationofsilvernanoparticlecoatedsurfacesbyldquotouchtestrdquomethod
AT toivakkam antimicrobialcharacterizationofsilvernanoparticlecoatedsurfacesbyldquotouchtestrdquomethod
AT makelajm antimicrobialcharacterizationofsilvernanoparticlecoatedsurfacesbyldquotouchtestrdquomethod
AT huovinenp antimicrobialcharacterizationofsilvernanoparticlecoatedsurfacesbyldquotouchtestrdquomethod
AT eerolae antimicrobialcharacterizationofsilvernanoparticlecoatedsurfacesbyldquotouchtestrdquomethod
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