Precise optical control of gene expression in C elegans using improved genetic code expansion and Cre recombinase

Synthetic strategies for optically controlling gene expression may enable the precise spatiotemporal control of genes in any combination of cells that cannot be targeted with specific promoters. We develop an improved genetic code expansion system in Caenorhabditis elegans and use it to create a pho...

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Autores principales:	Lloyd Davis, Inja Radman, Angeliki Goutou, Ailish Tynan, Kieran Baxter, Zhiyan Xi, Jack M O'Shea, Jason W Chin, Sebastian Greiss
Formato:	article
Lenguaje:	EN
Publicado:	eLife Sciences Publications Ltd 2021
Materias:	genetic code expansion non canonical amino acids optogenetics Cre recombinase photo-caged amino acid Medicine R Science Q Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/9ed4db72b5494942b206e4f1f417c6b3
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spelling	oai:doaj.org-article:9ed4db72b5494942b206e4f1f417c6b32021-11-15T07:05:36ZPrecise optical control of gene expression in C elegans using improved genetic code expansion and Cre recombinase10.7554/eLife.670752050-084Xe67075https://doaj.org/article/9ed4db72b5494942b206e4f1f417c6b32021-08-01T00:00:00Zhttps://elifesciences.org/articles/67075https://doaj.org/toc/2050-084XSynthetic strategies for optically controlling gene expression may enable the precise spatiotemporal control of genes in any combination of cells that cannot be targeted with specific promoters. We develop an improved genetic code expansion system in Caenorhabditis elegans and use it to create a photoactivatable Cre recombinase. We laser-activate Cre in single neurons within a bilaterally symmetric pair to selectively switch on expression of a loxP-controlled optogenetic channel in the targeted neuron. We use the system to dissect, in freely moving animals, the individual contributions of the mechanosensory neurons PLML/PLMR to the C. elegans touch response circuit, revealing distinct and synergistic roles for these neurons. We thus demonstrate how genetic code expansion and optical targeting can be combined to break the symmetry of neuron pairs and dissect behavioural outputs of individual neurons that cannot be genetically targeted.Lloyd DavisInja RadmanAngeliki GoutouAilish TynanKieran BaxterZhiyan XiJack M O'SheaJason W ChinSebastian GreisseLife Sciences Publications Ltdarticlegenetic code expansionnon canonical amino acidsoptogeneticsCre recombinasephoto-caged amino acidMedicineRScienceQBiology (General)QH301-705.5ENeLife, Vol 10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	genetic code expansion non canonical amino acids optogenetics Cre recombinase photo-caged amino acid Medicine R Science Q Biology (General) QH301-705.5
spellingShingle	genetic code expansion non canonical amino acids optogenetics Cre recombinase photo-caged amino acid Medicine R Science Q Biology (General) QH301-705.5 Lloyd Davis Inja Radman Angeliki Goutou Ailish Tynan Kieran Baxter Zhiyan Xi Jack M O'Shea Jason W Chin Sebastian Greiss Precise optical control of gene expression in C elegans using improved genetic code expansion and Cre recombinase
description	Synthetic strategies for optically controlling gene expression may enable the precise spatiotemporal control of genes in any combination of cells that cannot be targeted with specific promoters. We develop an improved genetic code expansion system in Caenorhabditis elegans and use it to create a photoactivatable Cre recombinase. We laser-activate Cre in single neurons within a bilaterally symmetric pair to selectively switch on expression of a loxP-controlled optogenetic channel in the targeted neuron. We use the system to dissect, in freely moving animals, the individual contributions of the mechanosensory neurons PLML/PLMR to the C. elegans touch response circuit, revealing distinct and synergistic roles for these neurons. We thus demonstrate how genetic code expansion and optical targeting can be combined to break the symmetry of neuron pairs and dissect behavioural outputs of individual neurons that cannot be genetically targeted.
format	article
author	Lloyd Davis Inja Radman Angeliki Goutou Ailish Tynan Kieran Baxter Zhiyan Xi Jack M O'Shea Jason W Chin Sebastian Greiss
author_facet	Lloyd Davis Inja Radman Angeliki Goutou Ailish Tynan Kieran Baxter Zhiyan Xi Jack M O'Shea Jason W Chin Sebastian Greiss
author_sort	Lloyd Davis
title	Precise optical control of gene expression in C elegans using improved genetic code expansion and Cre recombinase
title_short	Precise optical control of gene expression in C elegans using improved genetic code expansion and Cre recombinase
title_full	Precise optical control of gene expression in C elegans using improved genetic code expansion and Cre recombinase
title_fullStr	Precise optical control of gene expression in C elegans using improved genetic code expansion and Cre recombinase
title_full_unstemmed	Precise optical control of gene expression in C elegans using improved genetic code expansion and Cre recombinase
title_sort	precise optical control of gene expression in c elegans using improved genetic code expansion and cre recombinase
publisher	eLife Sciences Publications Ltd
publishDate	2021
url	https://doaj.org/article/9ed4db72b5494942b206e4f1f417c6b3
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Precise optical control of gene expression in C elegans using improved genetic code expansion and Cre recombinase

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