Ultrastructural study of collagen fibrils, proteoglycans and lamellae of the cornea treated with iontophoresis – UVA cross-linking and hypotonic riboflavin solution

To investigate the effects of iontophoresis–ultraviolet A (UVA) cross-linking (CXL) with hypotonic riboflavin solution on the ultrastructural changes in the lamellae, collagen fibrils (CFs), and proteoglycans (PGs) in the central and peripheral stroma of the human corneal buttons. The iontophoresis...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Turki Almubrad, Rita Mencucci, Adrian Smedowski, Ramachandran Samivel, Essam Almutleb, Aljoharah Alkanaan, Adnan Ali Khan, Ali Masmali, Saeed Akhtar
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Collagen fibrils Proteoglycans Cornea Iontophoresis Ultraviolet A Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/9ed4f0fa9d0441008d36cfc12e273864
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:9ed4f0fa9d0441008d36cfc12e273864
record_format	dspace
spelling	oai:doaj.org-article:9ed4f0fa9d0441008d36cfc12e2738642021-11-20T04:56:51ZUltrastructural study of collagen fibrils, proteoglycans and lamellae of the cornea treated with iontophoresis – UVA cross-linking and hypotonic riboflavin solution1319-562X10.1016/j.sjbs.2021.08.019https://doaj.org/article/9ed4f0fa9d0441008d36cfc12e2738642021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S1319562X21006902https://doaj.org/toc/1319-562XTo investigate the effects of iontophoresis–ultraviolet A (UVA) cross-linking (CXL) with hypotonic riboflavin solution on the ultrastructural changes in the lamellae, collagen fibrils (CFs), and proteoglycans (PGs) in the central and peripheral stroma of the human corneal buttons. The iontophoresis method was used for the trans-epithelial application of hypotonic riboflavin in ex vivo corneal culture for 5 min. The corneas were irradiated using three methods: Group 1 (G1), a UVA irradiance of 3 mW/cm2 for 30 min; Group 2 (G2), a UVA irradiance of 10 mW/cm2 for 9 min; Group 3 (G3), without UVA irradiation. Three untreated corneas were used as controls (G0). After the CXL procedure, the corneas were processed for electron microscopy. The CF diameter and PGs in each sample were analyzed using the iTEM program. The keratocyte organelles and stromal architecture in the peripheral cornea were better preserved than those in the central cornea. In G1 and G2, the mean CF diameter in the peripheral cornea was significantly higher than that in the central cornea. In G3, the CF diameter in the central cornea was significantly larger than that in the peripheral cornea. Furthermore, differences in PG area size were observed between the central and peripheral corneas in all groups. Riboflavin + UVA application at 3 mW/cm2 for 30 min and 10 mW/cm2 for 9 min was a suitable method of CXL; however, 3 mW/cm2 for 30 min improved the organization and size of the collagen fibrils. CXL treatment applied at the periphery was more effective than that applied at the center.Turki AlmubradRita MencucciAdrian SmedowskiRamachandran SamivelEssam AlmutlebAljoharah AlkanaanAdnan Ali KhanAli MasmaliSaeed AkhtarElsevierarticleCollagen fibrilsProteoglycansCorneaIontophoresisUltraviolet ABiology (General)QH301-705.5ENSaudi Journal of Biological Sciences, Vol 28, Iss 12, Pp 7160-7174 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Collagen fibrils Proteoglycans Cornea Iontophoresis Ultraviolet A Biology (General) QH301-705.5
spellingShingle	Collagen fibrils Proteoglycans Cornea Iontophoresis Ultraviolet A Biology (General) QH301-705.5 Turki Almubrad Rita Mencucci Adrian Smedowski Ramachandran Samivel Essam Almutleb Aljoharah Alkanaan Adnan Ali Khan Ali Masmali Saeed Akhtar Ultrastructural study of collagen fibrils, proteoglycans and lamellae of the cornea treated with iontophoresis – UVA cross-linking and hypotonic riboflavin solution
description	To investigate the effects of iontophoresis–ultraviolet A (UVA) cross-linking (CXL) with hypotonic riboflavin solution on the ultrastructural changes in the lamellae, collagen fibrils (CFs), and proteoglycans (PGs) in the central and peripheral stroma of the human corneal buttons. The iontophoresis method was used for the trans-epithelial application of hypotonic riboflavin in ex vivo corneal culture for 5 min. The corneas were irradiated using three methods: Group 1 (G1), a UVA irradiance of 3 mW/cm2 for 30 min; Group 2 (G2), a UVA irradiance of 10 mW/cm2 for 9 min; Group 3 (G3), without UVA irradiation. Three untreated corneas were used as controls (G0). After the CXL procedure, the corneas were processed for electron microscopy. The CF diameter and PGs in each sample were analyzed using the iTEM program. The keratocyte organelles and stromal architecture in the peripheral cornea were better preserved than those in the central cornea. In G1 and G2, the mean CF diameter in the peripheral cornea was significantly higher than that in the central cornea. In G3, the CF diameter in the central cornea was significantly larger than that in the peripheral cornea. Furthermore, differences in PG area size were observed between the central and peripheral corneas in all groups. Riboflavin + UVA application at 3 mW/cm2 for 30 min and 10 mW/cm2 for 9 min was a suitable method of CXL; however, 3 mW/cm2 for 30 min improved the organization and size of the collagen fibrils. CXL treatment applied at the periphery was more effective than that applied at the center.
format	article
author	Turki Almubrad Rita Mencucci Adrian Smedowski Ramachandran Samivel Essam Almutleb Aljoharah Alkanaan Adnan Ali Khan Ali Masmali Saeed Akhtar
author_facet	Turki Almubrad Rita Mencucci Adrian Smedowski Ramachandran Samivel Essam Almutleb Aljoharah Alkanaan Adnan Ali Khan Ali Masmali Saeed Akhtar
author_sort	Turki Almubrad
title	Ultrastructural study of collagen fibrils, proteoglycans and lamellae of the cornea treated with iontophoresis – UVA cross-linking and hypotonic riboflavin solution
title_short	Ultrastructural study of collagen fibrils, proteoglycans and lamellae of the cornea treated with iontophoresis – UVA cross-linking and hypotonic riboflavin solution
title_full	Ultrastructural study of collagen fibrils, proteoglycans and lamellae of the cornea treated with iontophoresis – UVA cross-linking and hypotonic riboflavin solution
title_fullStr	Ultrastructural study of collagen fibrils, proteoglycans and lamellae of the cornea treated with iontophoresis – UVA cross-linking and hypotonic riboflavin solution
title_full_unstemmed	Ultrastructural study of collagen fibrils, proteoglycans and lamellae of the cornea treated with iontophoresis – UVA cross-linking and hypotonic riboflavin solution
title_sort	ultrastructural study of collagen fibrils, proteoglycans and lamellae of the cornea treated with iontophoresis – uva cross-linking and hypotonic riboflavin solution
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/9ed4f0fa9d0441008d36cfc12e273864
work_keys_str_mv	AT turkialmubrad ultrastructuralstudyofcollagenfibrilsproteoglycansandlamellaeofthecorneatreatedwithiontophoresisuvacrosslinkingandhypotonicriboflavinsolution AT ritamencucci ultrastructuralstudyofcollagenfibrilsproteoglycansandlamellaeofthecorneatreatedwithiontophoresisuvacrosslinkingandhypotonicriboflavinsolution AT adriansmedowski ultrastructuralstudyofcollagenfibrilsproteoglycansandlamellaeofthecorneatreatedwithiontophoresisuvacrosslinkingandhypotonicriboflavinsolution AT ramachandransamivel ultrastructuralstudyofcollagenfibrilsproteoglycansandlamellaeofthecorneatreatedwithiontophoresisuvacrosslinkingandhypotonicriboflavinsolution AT essamalmutleb ultrastructuralstudyofcollagenfibrilsproteoglycansandlamellaeofthecorneatreatedwithiontophoresisuvacrosslinkingandhypotonicriboflavinsolution AT aljoharahalkanaan ultrastructuralstudyofcollagenfibrilsproteoglycansandlamellaeofthecorneatreatedwithiontophoresisuvacrosslinkingandhypotonicriboflavinsolution AT adnanalikhan ultrastructuralstudyofcollagenfibrilsproteoglycansandlamellaeofthecorneatreatedwithiontophoresisuvacrosslinkingandhypotonicriboflavinsolution AT alimasmali ultrastructuralstudyofcollagenfibrilsproteoglycansandlamellaeofthecorneatreatedwithiontophoresisuvacrosslinkingandhypotonicriboflavinsolution AT saeedakhtar ultrastructuralstudyofcollagenfibrilsproteoglycansandlamellaeofthecorneatreatedwithiontophoresisuvacrosslinkingandhypotonicriboflavinsolution
_version_	1718419731447283712

Ultrastructural study of collagen fibrils, proteoglycans and lamellae of the cornea treated with iontophoresis – UVA cross-linking and hypotonic riboflavin solution

Ejemplares similares