Expression profiling of human milk derived exosomal microRNAs and their targets in HIV-1 infected mothers

Abstract Despite the use of antiretroviral therapy (ART) in HIV-1 infected mothers approximately 5% of new HIV-1 infections still occur in breastfed infants annually, which warrants for the development of novel strategies to prevent new HIV-1 infections in infants. Human milk (HM) exosomes are highl...

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Autores principales:	Muhammad Atif Zahoor, Xiao-Dan Yao, Bethany M. Henrick, Chris P. Verschoor, Alash’le Abimiku, Sophia Osawe, Kenneth L. Rosenthal
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9ed61c01845e46c5ae59c1e0b7d187ff
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spelling	oai:doaj.org-article:9ed61c01845e46c5ae59c1e0b7d187ff2021-12-02T16:06:39ZExpression profiling of human milk derived exosomal microRNAs and their targets in HIV-1 infected mothers10.1038/s41598-020-69799-x2045-2322https://doaj.org/article/9ed61c01845e46c5ae59c1e0b7d187ff2020-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-69799-xhttps://doaj.org/toc/2045-2322Abstract Despite the use of antiretroviral therapy (ART) in HIV-1 infected mothers approximately 5% of new HIV-1 infections still occur in breastfed infants annually, which warrants for the development of novel strategies to prevent new HIV-1 infections in infants. Human milk (HM) exosomes are highly enriched in microRNAs (miRNAs), which play an important role in neonatal immunity. Furthermore, HM exosomes from healthy donors are known to inhibit HIV-1 infection and transmission; however, the effect of HIV-1 on HM exosomal miRNA signatures remains unknown. In this study, we used nCounter NanoString technology and investigated miRNAs expression profiles in first week postpartum HM exosomes from HIV-1 infected and uninfected control mothers (n = 36). Our results indicated that HIV-1 perturbed the differential expression patterns of 19 miRNAs (13 upregulated and 6 downregulated) in HIV-1 infected women compared to healthy controls. DIANA-miR functional pathway analyses revealed that multiple biological pathways are involved including cell cycle, pathways in cancer, TGF-β signaling, FoxO signaling, fatty acid biosynthesis, p53 signaling and apoptosis. Moreover, the receiver operating characteristics (ROC) curve analyses of miR-630 and miR-378g yielded areas under the ROC curves of 0.82 (95% CI 0.67 to 0.82) and 0.83 (95% CI 0.67 to 0.83), respectively highlighting their potential to serve as biomarkers to identify HIV-1 infection in women. These data may contribute to the development of new therapeutic strategies in prevention of mother-to-child transmission (MTCT) of HIV-1.Muhammad Atif ZahoorXiao-Dan YaoBethany M. HenrickChris P. VerschoorAlash’le AbimikuSophia OsaweKenneth L. RosenthalNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-16 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Muhammad Atif Zahoor Xiao-Dan Yao Bethany M. Henrick Chris P. Verschoor Alash’le Abimiku Sophia Osawe Kenneth L. Rosenthal Expression profiling of human milk derived exosomal microRNAs and their targets in HIV-1 infected mothers
description	Abstract Despite the use of antiretroviral therapy (ART) in HIV-1 infected mothers approximately 5% of new HIV-1 infections still occur in breastfed infants annually, which warrants for the development of novel strategies to prevent new HIV-1 infections in infants. Human milk (HM) exosomes are highly enriched in microRNAs (miRNAs), which play an important role in neonatal immunity. Furthermore, HM exosomes from healthy donors are known to inhibit HIV-1 infection and transmission; however, the effect of HIV-1 on HM exosomal miRNA signatures remains unknown. In this study, we used nCounter NanoString technology and investigated miRNAs expression profiles in first week postpartum HM exosomes from HIV-1 infected and uninfected control mothers (n = 36). Our results indicated that HIV-1 perturbed the differential expression patterns of 19 miRNAs (13 upregulated and 6 downregulated) in HIV-1 infected women compared to healthy controls. DIANA-miR functional pathway analyses revealed that multiple biological pathways are involved including cell cycle, pathways in cancer, TGF-β signaling, FoxO signaling, fatty acid biosynthesis, p53 signaling and apoptosis. Moreover, the receiver operating characteristics (ROC) curve analyses of miR-630 and miR-378g yielded areas under the ROC curves of 0.82 (95% CI 0.67 to 0.82) and 0.83 (95% CI 0.67 to 0.83), respectively highlighting their potential to serve as biomarkers to identify HIV-1 infection in women. These data may contribute to the development of new therapeutic strategies in prevention of mother-to-child transmission (MTCT) of HIV-1.
format	article
author	Muhammad Atif Zahoor Xiao-Dan Yao Bethany M. Henrick Chris P. Verschoor Alash’le Abimiku Sophia Osawe Kenneth L. Rosenthal
author_facet	Muhammad Atif Zahoor Xiao-Dan Yao Bethany M. Henrick Chris P. Verschoor Alash’le Abimiku Sophia Osawe Kenneth L. Rosenthal
author_sort	Muhammad Atif Zahoor
title	Expression profiling of human milk derived exosomal microRNAs and their targets in HIV-1 infected mothers
title_short	Expression profiling of human milk derived exosomal microRNAs and their targets in HIV-1 infected mothers
title_full	Expression profiling of human milk derived exosomal microRNAs and their targets in HIV-1 infected mothers
title_fullStr	Expression profiling of human milk derived exosomal microRNAs and their targets in HIV-1 infected mothers
title_full_unstemmed	Expression profiling of human milk derived exosomal microRNAs and their targets in HIV-1 infected mothers
title_sort	expression profiling of human milk derived exosomal micrornas and their targets in hiv-1 infected mothers
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/9ed61c01845e46c5ae59c1e0b7d187ff
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Expression profiling of human milk derived exosomal microRNAs and their targets in HIV-1 infected mothers

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