OhrR of Mycobacterium smegmatis senses and responds to intracellular organic hydroperoxide stress

Abstract Organic hydroperoxide reductase regulator (OhrR) in bacteria is a sensor for organic hydroperoxide stress and a transcriptional regulator for the enzyme organic hydroperoxide reductase (Ohr). In this study we investigated, using a GFP reporter system, whether Mycobacterium smegmatis OhrR ha...

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Autores principales:	Omar A. Garnica, Kishore Das, Subramanian Dhandayuthapani
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9ed85bc954994bb2ab572db0149b2de2
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spelling	oai:doaj.org-article:9ed85bc954994bb2ab572db0149b2de22021-12-02T16:07:00ZOhrR of Mycobacterium smegmatis senses and responds to intracellular organic hydroperoxide stress10.1038/s41598-017-03819-12045-2322https://doaj.org/article/9ed85bc954994bb2ab572db0149b2de22017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03819-1https://doaj.org/toc/2045-2322Abstract Organic hydroperoxide reductase regulator (OhrR) in bacteria is a sensor for organic hydroperoxide stress and a transcriptional regulator for the enzyme organic hydroperoxide reductase (Ohr). In this study we investigated, using a GFP reporter system, whether Mycobacterium smegmatis OhrR has the ability to sense and respond to intracellular organic hydroperoxide stress. It was observed that M. smegmatis strains bearing the pohr-gfpuv fusion construct were able to express GFP only in the absence of an intact ohrR gene, but not in its presence. However, GFP expression in the strain bearing pohr-gfpuv with an intact ohrR gene could be induced by organic hydroperoxides in vitro and in the intracellular environment upon ingestion of the bacteria by macrophages; indicating that OhrR responds not only to in vitro but also to intracellular organic hydroperoxide stress. Further, the intracellular expression of pohr driven GFP in this strain could be abolished by replacing the intact ohrR gene with a mutant ohrR gene modified for N-terminal Cysteine (Cys) residue, suggesting that OhrR senses intracellular organic hydroperoxides through Cys residue. This is the first report demonstrating the ability of OhrR to sense intracellular organic hydroperoxides.Omar A. GarnicaKishore DasSubramanian DhandayuthapaniNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Omar A. Garnica Kishore Das Subramanian Dhandayuthapani OhrR of Mycobacterium smegmatis senses and responds to intracellular organic hydroperoxide stress
description	Abstract Organic hydroperoxide reductase regulator (OhrR) in bacteria is a sensor for organic hydroperoxide stress and a transcriptional regulator for the enzyme organic hydroperoxide reductase (Ohr). In this study we investigated, using a GFP reporter system, whether Mycobacterium smegmatis OhrR has the ability to sense and respond to intracellular organic hydroperoxide stress. It was observed that M. smegmatis strains bearing the pohr-gfpuv fusion construct were able to express GFP only in the absence of an intact ohrR gene, but not in its presence. However, GFP expression in the strain bearing pohr-gfpuv with an intact ohrR gene could be induced by organic hydroperoxides in vitro and in the intracellular environment upon ingestion of the bacteria by macrophages; indicating that OhrR responds not only to in vitro but also to intracellular organic hydroperoxide stress. Further, the intracellular expression of pohr driven GFP in this strain could be abolished by replacing the intact ohrR gene with a mutant ohrR gene modified for N-terminal Cysteine (Cys) residue, suggesting that OhrR senses intracellular organic hydroperoxides through Cys residue. This is the first report demonstrating the ability of OhrR to sense intracellular organic hydroperoxides.
format	article
author	Omar A. Garnica Kishore Das Subramanian Dhandayuthapani
author_facet	Omar A. Garnica Kishore Das Subramanian Dhandayuthapani
author_sort	Omar A. Garnica
title	OhrR of Mycobacterium smegmatis senses and responds to intracellular organic hydroperoxide stress
title_short	OhrR of Mycobacterium smegmatis senses and responds to intracellular organic hydroperoxide stress
title_full	OhrR of Mycobacterium smegmatis senses and responds to intracellular organic hydroperoxide stress
title_fullStr	OhrR of Mycobacterium smegmatis senses and responds to intracellular organic hydroperoxide stress
title_full_unstemmed	OhrR of Mycobacterium smegmatis senses and responds to intracellular organic hydroperoxide stress
title_sort	ohrr of mycobacterium smegmatis senses and responds to intracellular organic hydroperoxide stress
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/9ed85bc954994bb2ab572db0149b2de2
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OhrR of Mycobacterium smegmatis senses and responds to intracellular organic hydroperoxide stress

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